##################################
# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI Alw44I AlwI
Aor51HI ApaI ApaLI AscI Asp718I
AspHI AvaI AviII AvrII BalI
BamHI BanII BbeI BbrPI BbvCI
BcgI BciVI BcnI BfrI BglI
BlnI BmyI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsoBI Bsp1286I BspDI
BspEI BspHI BsrBI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstXI
BstZ17I Cac8I CfoI ClaI CpoI
DraIII DrdI EaeI EagI EclXI
Eco47III Eco52I EcoRV EcoT22I FauI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmlI
PshAI Psp1406I PvuI RcaI RsrII
SacI SacII SapI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StuI XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 113 GTCGAC GT^[AC][GT]AC
AciI 19 GCGG CCGC(-3/-1)
AhdI 1964 GACTTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1015 CCTAAGG CC^T[ACGT]AGG
AseI 2299 ATTAAT AT^TAAT
AsnI 2299 ATTAAT AT^TAAT
Asp700I 1002 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1964 GACTTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 617 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 813 GGTGCC G^G[CT][GA]CC
BseRI 2547 GAGGAG GAGGAG(10/8)
BsgI 673 GTGCAG GTGCAG(16/14)
BsrFI 2086 GCCGGT [GA]^CCGG[CT]
BstEII 2587 GGTAACC G^GT[ACGT]ACC
BstPI 2587 GGTAACC G^GT[ACGT]ACC
Bsu36I 1015 CCTAAGG CC^T[ACGT]AGG
Cfr10I 2086 GCCGGT [GA]^CCGG[CT]
DsaI 1371 CCATGG C^C[GA][CT]GG
Eam1105I 1964 GACTTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1015 CCTAAGG CC^T[ACGT]AGG
EcoNI 1518 CCTTATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2587 GGTAACC G^GT[ACGT]ACC
HapII 2087 CCGG C^CGG
HpaII 2087 CCGG C^CGG
MaeII 691 ACGT A^CGT
MspA1I 2539 CAGCTG C[AC]G^C[GT]G
MspI 2087 CCGG C^CGG
NcoI 1371 CCATGG C^CATGG
PflFI 617 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1036 CCACTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmeI 1533 GTTTAAAC GTTT^AAAC
PpuMI 1239 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 2299 ATTAAT AT^TAAT
PstI 2520 CTGCAG CTGCA^G
PvuII 2539 CAGCTG CAG^CTG
SalI 113 GTCGAC G^TCGAC
ScaI 133 AGTACT AGT^ACT
SwaI 1301 ATTTAAAT ATTT^AAAT
Tsp45I 775 GTCAC ^GT[GC]AC
Tth111I 617 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1036 CCACTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 560 CCAAAGACCAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1002 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflIII 1266 ACATGT A^C[GA][CT]GT
2112 ACATGT
BclI 351 TGATCA T^GATCA
1670 TGATCA
BglII 1482 AGATCT A^GATCT
1602 AGATCT
BlpI 508 GCTGAGC GC^T[ACGT]AGC
1360 GCTCAGC
BpmI 1399 CTCCAG CTGGAG(16/14)
2542 CTGGAG
Bpu1102I 508 GCTGAGC GC^T[ACGT]AGC
1360 GCTCAGC
BsmFI 1442 GGGAC GGGAC(10/14)
1327 GTCCC
Bsp1407I 1597 TGTACA T^GTACA
2812 TGTACA
BspLU11I 1266 ACATGT A^CATGT
2112 ACATGT
BspMI 831 ACCTGC ACCTGC(4/8)
732 GCAGGT
BsrGI 1597 TGTACA T^GTACA
2812 TGTACA
BstYI 1482 AGATCT [GA]^GATC[CT]
1602 AGATCT
CelII 508 GCTGAGC GC^T[ACGT]AGC
1360 GCTCAGC
DraII 1239 GGGTCCT [GA]G^G[ACGT]CC[CT]
1983 AGGCCCT
EarI 1974 CTCTTC CTCTTC(1/4)
2270 CTCTTC
EcoO109I 1239 GGGTCCT [GA]G^G[ACGT]CC[CT]
1983 AGGCCCT
FbaI 351 TGATCA T^GATCA
1670 TGATCA
HincII 113 GTCGAC GT[CT]^[GA]AC
2017 GTCAAC
HindII 113 GTCGAC GT[CT]^[GA]AC
2017 GTCAAC
Ksp632I 1974 CTCTTC CTCTTC(1/4)
2270 CTCTTC
MflI 1482 AGATCT [GA]^GATC[CT]
1602 AGATCT
SexAI 618 ACCTGGT A^CC[AT]GGT
2508 ACCAGGT
SfcI 2520 CTGCAG C^T[GA][CT]AG
2729 CTATAG
SspBI 1597 TGTACA T^GTACA
2812 TGTACA
SspI 438 AATATT AAT^ATT
2748 AATATT
XhoII 1482 AGATCT [GA]^GATC[CT]
1602 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AcsI AfaI AluI AlwNI ApoI
AvaII BbsI BbvI BfaI BpuAI
BsaJI BsiYI BslI BsmAI BsmI
BsrDI BsrI BssKI BstF5I BstNI
Cfr13I DdeI DpnII DraI EcoRI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeI MaeIII
MboI MboII MnlI MseI MslI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI StyI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I