##################################
# NO CUT ENZYMES (  106 enzymes) #
##################################

AatII       AccIII      AclI        AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        AspEI       AvaII       
AviII       AvrII       BanII       BbeI        BbrPI       
BbvCI       BciVI       BglI        BglII       BlnI        
BsaAI       BsaBI       BsaWI       BseAI       BsiWI       
BsmBI       BspDI       BspEI       BsrBI       BsrFI       
BssHII      BssSI       BstAPI      BstBI       BstEII      
BstPI       Bsu36I      CfoI        Cfr10I      ClaI        
CpoI        DraIII      DrdI        Eam1105I    Eco47III    
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
FauI        FseI        FspI        HaeII       HhaI        
HinP1I      KasI        KspI        MamI        MfeI        
MluI        MroI        MslI        MunI        NaeI        
NarI        NdeI        NgoMIV      NheI        NruI        
NspV        PacI        PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshBI       Psp1406I    
PvuI        RsrII       SacI        SacII       SalI        
SbfI        ScaI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspI        SwaI        Van91I      XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

Acc65I        638  GGTACC               G^GTACC
AccII          19  CGCG                 CG^CG
AcyI         4427  GGCGTC               G[GA]^CG[CT]C
Asp718I       638  GGTACC               G^GTACC
AspI         3878  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          325  CTCGAG               C^[CT]CG[GA]G
BalI         1651  TGGCCA               TGG^CCA
BamHI         868  GGATCC               G^GATCC
BanI          638  GGTACC               G^G[CT][GA]CC
BcgI          555  GCAGCTGCCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaHI        4427  GGCGTC               G[GA]^CG[CT]C
BsoBI         325  CTCGAG               C^[CT]CG[GA]G
Bst1107I     1363  GTATAC               GTA^TAC
BstUI          19  CGCG                 CG^CG
BstXI         872  CCAGAAATCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      1363  GTATAC               GTA^TAC
Cfr13I       2048  GGCCC                G^G[ACGT]CC
DraII        2047  AGGCCCT              [GA]G^G[ACGT]CC[CT]
DsaI           82  CCATGG               C^C[GA][CT]GG
EcoO109I     2047  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoT22I      2288  ATGCAT               ATGCA^T
Hin1I        4427  GGCGTC               G[GA]^CG[CT]C
HincII       1154  GTTAAC               GT[CT]^[GA]AC
HindII       1154  GTTAAC               GT[CT]^[GA]AC
HpaI         1154  GTTAAC               GTT^AAC
KpnI          638  GGTACC               GGTAC^C
MaeII        1961  ACGT                 A^CGT
MluNI        1651  TGGCCA               TGG^CCA
MscI         1651  TGGCCA               TGG^CCA
MvnI           19  CGCG                 CG^CG
NcoI           82  CCATGG               C^CATGG
NotI           14  GCGGCCGC             GC^GGCCGC
NsiI         2288  ATGCAT               ATGCA^T
PaeR7I        325  CTCGAG               C^TCGAG
PflFI        3878  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI         804  GACTCAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI         1417  CTGCAG               CTGCA^G
Sau96I       2048  GGCCC                G^G[ACGT]CC
SexAI        3970  ACCAGGT              A^CC[AT]GGT
StuI         2845  AGGCCT               AGG^CCT
Tth111I      3878  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         3347  TCTAGA               T^CTAGA
XcmI         2492  CCATGAGAAAGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          325  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   39 enzymes) #
###################################

AflII        1992  CTTAAG               C^TTAAG
             2478  CTTAAG              
AspHI        3351  GAGCAC               G[AT]GC[AT]^C
             3963  GAGCAC              
BclI          365  TGATCA               T^GATCA
             2444  TGATCA              
BcnI           27  CCCGG                CC^[GC]GG
               40  CCCGG               
BfrI         1992  CTTAAG               C^TTAAG
             2478  CTTAAG              
BlpI         1518  GCTGAGC              GC^T[ACGT]AGC
             2433  GCTAAGC             
BmyI         3351  GAGCAC               G[AGT]GC[ACT]^C
             3963  GAGCAC              
Bpu1102I     1518  GCTGAGC              GC^T[ACGT]AGC
             2433  GCTAAGC             
BsaI           30  GGTCTC               GGTCTC(1/5)
             3111  GGTCTC              
BseRI        2370  GAGGAG               GAGGAG(10/8)
             3333  GAGGAG              
BsgI         2074  CTGCAC               GTGCAG(16/14)
             2034  GTGCAG              
BsiEI          15  CGGCCG               CG[GA][CT]^CG
               42  CGGCCG              
BsiHKAI      3351  GAGCAC               G[AT]GC[AT]^C
             3963  GAGCAC              
BsiYI        2755  CCAATACAGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             3851  CCCAGAAACGG         
BslI         2755  CCAATACAGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             3851  CCCAGAAACGG         
Bsp1286I     3351  GAGCAC               G[AGT]GC[ACT]^C
             3963  GAGCAC              
Bsp1407I     2978  TGTACA               T^GTACA
             4331  TGTACA              
BspHI         166  TCATGA               T^CATGA
             3062  TCATGA              
BspMI         464  GCAGGT               ACCTGC(4/8)
             1790  GCAGGT              
BsrDI         661  CATTGC               GCAATG(2/0)
             1189  GCAATG              
BsrGI        2978  TGTACA               T^GTACA
             4331  TGTACA              
BstYI         868  GGATCC               [GA]^GATC[CT]
             2777  GGATCT              
CelII        1518  GCTGAGC              GC^T[ACGT]AGC
             2433  GCTAAGC             
EagI           15  CGGCCG               C^GGCCG
               42  CGGCCG              
EclXI          15  CGGCCG               C^GGCCG
               42  CGGCCG              
Eco52I         15  CGGCCG               C^GGCCG
               42  CGGCCG              
EcoT14I        82  CCATGG               C^C[AT][AT]GG
             1699  CCTTGG              
FbaI          365  TGATCA               T^GATCA
             2444  TGATCA              
HapII          28  CCGG                 C^CGG
               41  CCGG                
HpaII          28  CCGG                 C^CGG
               41  CCGG                
MflI          868  GGATCC               [GA]^GATC[CT]
             2777  GGATCT              
MspI           28  CCGG                 C^CGG
               41  CCGG                
NciI           27  CCCGG                CC^[GC]GG
               40  CCCGG               
NlaIV         638  GGTACC               GG[ACGT]^[ACGT]CC
              868  GGATCC              
PvuII         556  CAGCTG               CAG^CTG
             2964  CAGCTG              
RcaI          166  TCATGA               T^CATGA
             3062  TCATGA              
SspBI        2978  TGTACA               T^GTACA
             4331  TGTACA              
StyI           82  CCATGG               C^C[AT][AT]GG
             1699  CCTTGG              
XhoII         868  GGATCC               [GA]^GATC[CT]
             2777  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   69 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AflIII      
AluI        AlwI        AlwNI       ApoI        Asp700I     
BbsI        BbvI        BfaI        BpmI        BpuAI       
BsaJI       BsmAI       BsmFI       BsmI        BspLU11I    
BsrI        BssKI       BstF5I      BstNI       Cac8I       
DdeI        DpnII       DraI        EaeI        EarI        
EcoRII      Fnu4HI      FokI        HaeIII      HgaI        
HindIII     HinfI       HphI        ItaI        Ksp632I     
MaeI        MaeIII      MboI        MboII       MnlI        
MseI        MspA1I      MvaI        MwoI        NdeII       
NlaIII      NspI        PleI        RsaI        SapI        
Sau3AI      ScrFI       SfaNI       SfcI        SmlI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XmnI