##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
Acc65I AccIII AflII AflIII AgeI
AhdI Alw44I ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AviII
BanI BbeI BbrPI BcgI BciVI
BfrI BglII BsaAI BsaBI BsaWI
BseAI BsiWI BsmBI BspDI BspEI
BspLU11I BsrFI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstZ17I Cfr10I
ClaI CpoI DraIII DrdI Eam1105I
EcoO65I EcoRV FseI FspI KasI
KpnI KspI MamI MfeI MluI
MroI MunI NaeI NarI NgoMIV
NheI NotI NruI PacI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI PvuI RsrII SacII SalI
SbfI SfoI SgrAI SmaI SnaBI
SpeI Sse8387I SwaI Van91I XcmI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 943 GACGTC GACGT^C
AccII 1369 CGCG CG^CG
AclI 3251 AACGTT AA^CGTT
AcyI 943 GACGTC G[GA]^CG[CT]C
Aor51HI 1769 AGCGCT AGC^GCT
AspI 2170 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1315 CTCGAG C^[CT]CG[GA]G
AvrII 569 CCTAGG C^CTAGG
BamHI 2522 GGATCC G^GATCC
BbvCI 1881 GCTGAGG CCTCAGC(-5/-2)
BglI 985 GCCGTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 569 CCTAGG C^CTAGG
BsaHI 943 GACGTC G[GA]^CG[CT]C
BsiEI 3331 CGGCCG CG[GA][CT]^CG
BsmI 736 GCATTC GAATGC(1/-1)
BsoBI 1315 CTCGAG C^[CT]CG[GA]G
Bsp1407I 3577 TGTACA T^GTACA
BsrBI 1132 CCGCTC CCGCTC(-3/-3)
BsrGI 3577 TGTACA T^GTACA
BstBI 318 TTCGAA TT^CGAA
BstUI 1369 CGCG CG^CG
EagI 3331 CGGCCG C^GGCCG
EclXI 3331 CGGCCG C^GGCCG
Eco47III 1769 AGCGCT AGC^GCT
Eco52I 3331 CGGCCG C^GGCCG
EcoT22I 1606 ATGCAT ATGCA^T
FauI 1367 CCCGC CCCGC(4/6)
HaeII 1769 AGCGCT [GA]GCGC^[CT]
Hin1I 943 GACGTC G[GA]^CG[CT]C
HindIII 2107 AAGCTT A^AGCTT
MvnI 1369 CGCG CG^CG
NdeI 304 CATATG CA^TATG
NsiI 1606 ATGCAT ATGCA^T
NspV 318 TTCGAA TT^CGAA
PaeR7I 1315 CTCGAG C^TCGAG
PflFI 2170 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 3251 AACGTT AA^CGTT
SapI 2505 GAAGAGC GCTCTTC(1/4)
SexAI 3428 ACCTGGT A^CC[AT]GGT
SfiI 984 GGCCGTGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 318 TTCGAA TT^CGAA
SphI 1604 GCATGC GCATG^C
SspBI 3577 TGTACA T^GTACA
StuI 1405 AGGCCT AGG^CCT
Tth111I 2170 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2322 TCTAGA T^CTAGA
XhoI 1315 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 794 GTAGAC GT^[AC][GT]AC
2299 GTAGAC
AocI 1445 CCTCAGG CC^T[ACGT]AGG
2712 CCTAAGG
Asp700I 1593 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3151 GAAACACTTC
BalI 1328 TGGCCA TGG^CCA
3422 TGGCCA
BcnI 2519 CCCGG CC^[GC]GG
3334 CCGGG
BsaI 3294 GAGACC GGTCTC(1/5)
3412 GAGACC
BspHI 146 TCATGA T^CATGA
869 TCATGA
BspMI 169 ACCTGC ACCTGC(4/8)
492 GCAGGT
BstYI 2522 GGATCC [GA]^GATC[CT]
2922 GGATCT
Bsu36I 1445 CCTCAGG CC^T[ACGT]AGG
2712 CCTAAGG
DraII 1808 AGGTCCT [GA]G^G[ACGT]CC[CT]
2491 AGGACCC
DsaI 934 CCATGG C^C[GA][CT]GG
1357 CCATGG
Eco81I 1445 CCTCAGG CC^T[ACGT]AGG
2712 CCTAAGG
EcoNI 170 CCTGCAATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2031 CCTATCAGAGG
EcoO109I 1808 AGGTCCT [GA]G^G[ACGT]CC[CT]
2491 AGGACCC
EcoRI 340 GAATTC G^AATTC
1145 GAATTC
HgaI 1011 GACGC GACGC(5/10)
3516 GACGC
HpaI 637 GTTAAC GTT^AAC
2184 GTTAAC
MflI 2522 GGATCC [GA]^GATC[CT]
2922 GGATCT
MluNI 1328 TGGCCA TGG^CCA
3422 TGGCCA
MscI 1328 TGGCCA TGG^CCA
3422 TGGCCA
NciI 2519 CCCGG CC^[GC]GG
3334 CCGGG
NcoI 934 CCATGG C^CATGG
1357 CCATGG
NspI 1604 GCATGC [GA]CATG^[CT]
1608 GCATGT
PleI 3604 GACTC GAGTC(4/5)
1510 GAGTC
PpuMI 1808 AGGTCCT [GA]G^G[AT]CC[CT]
2491 AGGACCC
RcaI 146 TCATGA T^CATGA
869 TCATGA
SacI 998 GAGCTC GAGCT^C
3130 GAGCTC
XhoII 2522 GGATCC [GA]^GATC[CT]
2922 GGATCT
XmnI 1593 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3151 GAAACACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BanII
BbsI BbvI BclI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstXI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI EaeI EarI EcoRII EcoT14I
FbaI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV PstI
PvuII RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I