##################################
# NO CUT ENZYMES (   85 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AgeI        
AhdI        Aor51HI     AscI        Asp718I     AspEI       
AviII       BalI        BamHI       BbrPI       BcgI        
BclI        BglI        BsaAI       BsgI        BsiEI       
BsiWI       BsmBI       BspHI       BspMI       BsrBI       
BsrFI       BssHII      BssSI       BstBI       BstEII      
BstPI       BstUI       BstXI       Cfr10I      CpoI        
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoO65I     FauI        FbaI        FseI        
FspI        HpaI        KpnI        KspI        MluI        
MluNI       MscI        MvnI        NaeI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PinAI       PmaCI       PmeI        PmlI        PshAI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SacII       SapI        SbfI        SexAI       SfiI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    StuI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   62 enzymes) #
###################################

AccIII       2585  TCCGGA               T^CCGGA
AciI         1306  GCGG                 CCGC(-3/-1)
Alw44I       1115  GTGCAC               G^TGCAC
AocI         3008  CCTGAGG              CC^T[ACGT]AGG
ApaI          994  GGGCCC               GGGCC^C
ApaLI        1115  GTGCAC               G^TGCAC
Asp700I      2356  GAATTAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         3717  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         1169  CTCGAG               C^[CT]CG[GA]G
AvrII        1951  CCTAGG               C^CTAGG
BbeI           44  GGCGCC               GGCGC^C
BbvCI        3495  GCTGAGG              CCTCAGC(-5/-2)
BglII        1975  AGATCT               A^GATCT
BlnI         1951  CCTAGG               C^CTAGG
BlpI         1739  GCTTAGC              GC^T[ACGT]AGC
Bpu1102I     1739  GCTTAGC              GC^T[ACGT]AGC
BsaI            4  GAGACC               GGTCTC(1/5)
BsaWI        2585  TCCGGA               [AT]^CCGG[AT]
BseAI        2585  TCCGGA               T^CCGGA
BsmFI        3434  GGGAC                GGGAC(10/14)
BsoBI        1169  CTCGAG               C^[CT]CG[GA]G
BspDI         413  ATCGAT               AT^CGAT
BspEI        2585  TCCGGA               T^CCGGA
BspLU11I     2696  ACATGT               A^CATGT
Bst1107I     3962  GTATAC               GTA^TAC
BstAPI        253  GCAAATGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I      3962  GTATAC               GTA^TAC
Bsu36I       3008  CCTGAGG              CC^T[ACGT]AGG
Cac8I         717  GCGAGC               GC[ACGT]^[ACGT]GC
CelII        1739  GCTTAGC              GC^T[ACGT]AGC
CfoI           45  GCGC                 GCG^C
ClaI          413  ATCGAT               AT^CGAT
EaeI           38  TGGCCG               [CT]^GGCC[GA]
Eco81I       3008  CCTGAGG              CC^T[ACGT]AGG
EcoNI        2107  CCTCCTTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV        1914  GATATC               GAT^ATC
EcoT22I      1175  ATGCAT               ATGCA^T
HaeII          44  GGCGCC               [GA]GCGC^[CT]
HhaI           45  GCGC                 GCG^C
HinP1I         45  GCGC                 G^CGC
HindIII      1129  AAGCTT               A^AGCTT
KasI           44  GGCGCC               G^GCGCC
MfeI         1871  CAATTG               C^AATTG
MroI         2585  TCCGGA               T^CCGGA
MslI         1752  CACAGGTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I       1304  CAGCGG               C[AC]G^C[GT]G
MunI         1871  CAATTG               C^AATTG
NarI           44  GGCGCC               GG^CGCC
NcoI         2069  CCATGG               C^CATGG
NsiI         1175  ATGCAT               ATGCA^T
PaeR7I       1169  CTCGAG               C^TCGAG
PflFI        3717  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1777  CCAACTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         852  AGGACCC              [GA]G^G[AT]CC[CT]
ScaI          639  AGTACT               AGT^ACT
SfoI           44  GGCGCC               GGC^GCC
SwaI          407  ATTTAAAT             ATTT^AAAT
Tth111I      3717  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1777  CCAACTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         3197  TCTAGA               T^CTAGA
XhoI         1169  CTCGAG               C^TCGAG
XmnI         2356  GAATTAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AcyI           44  GGCGCC               G[GA]^CG[CT]C
             3988  GACGCC              
AflII         139  CTTAAG               C^TTAAG
             4096  CTTAAG              
AlwNI        1304  CAGCGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             3203  CAGGCCCTG           
AseI         2358  ATTAAT               AT^TAAT
             3072  ATTAAT              
AsnI         2358  ATTAAT               AT^TAAT
             3072  ATTAAT              
BanI           44  GGCGCC               G^G[CT][GA]CC
             1339  GGCACC              
BciVI         359  GTATCC               GTATCC(6/5)
             1142  GTATCC              
BcnI           41  CCGGG                CC^[GC]GG
             2146  CCCGG               
BfrI          139  CTTAAG               C^TTAAG
             4096  CTTAAG              
BsaHI          44  GGCGCC               G[GA]^CG[CT]C
             3988  GACGCC              
BseRI        2105  CTCCTC               GAGGAG(10/8)
             2738  GAGGAG              
Bsp1407I      275  TGTACA               T^GTACA
             1092  TGTACA              
BsrDI        2444  CATTGC               GCAATG(2/0)
             2782  GCAATG              
BsrGI         275  TGTACA               T^GTACA
             1092  TGTACA              
BstYI         825  AGATCC               [GA]^GATC[CT]
             1975  AGATCT              
DraIII       1388  CACGTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2603  CACCTTGTG           
Hin1I          44  GGCGCC               G[GA]^CG[CT]C
             3988  GACGCC              
MflI          825  AGATCC               [GA]^GATC[CT]
             1975  AGATCT              
NciI           41  CCGGG                CC^[GC]GG
             2146  CCCGG               
PshBI        2358  ATTAAT               AT^TAAT
             3072  ATTAAT              
SacI         2792  GAGCTC               GAGCT^C
             3030  GAGCTC              
SalI          911  GTCGAC               G^TCGAC
             3985  GTCGAC              
SspBI         275  TGTACA               T^GTACA
             1092  TGTACA              
XcmI          529  CCAGAATATCAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              953  CCAAAACTGGAATGG     
XhoII         825  AGATCC               [GA]^GATC[CT]
             1975  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   86 enzymes) #
#########################################

AccI        AcsI        AfaI        AflIII      AluI        
AlwI        ApoI        AspHI       AvaII       BanII       
BbsI        BbvI        BfaI        BmyI        BpmI        
BpuAI       BsaBI       BsaJI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmI        Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       Cfr13I      DdeI        
DpnII       DraI        DraII       DsaI        EarI        
EcoO109I    EcoRI       EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HgaI        HincII      
HindII      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MamI        
MboI        MboII       MnlI        MseI        MspI        
MvaI        MwoI        NdeI        NdeII       NlaIII      
NlaIV       NspI        PleI        PstI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SspI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I