##################################
# NO CUT ENZYMES (   74 enzymes) #
##################################

Acc65I      AccIII      AclI        AflII       AgeI        
AhdI        AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AviII       BbrPI       BbvCI       
BciVI       BclI        BfrI        BsaBI       BseAI       
BsiWI       BsmI        BspDI       BspEI       BspLU11I    
BssHII      Bst1107I    BstBI       BstZ17I     ClaI        
CpoI        Eam1105I    EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HindIII     KpnI        
KspI        MamI        MfeI        MluI        MroI        
MunI        NcoI        NdeI        NheI        NotI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    RsrII       SacII       SalI        
SapI        SfuI        SnaBI       SpeI        SspI        
StuI        SwaI        Tth111I     XhoI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AatII        2074  GACGTC               GACGT^C
AccII        1778  CGCG                 CG^CG
AcsI         1018  AAATTC               [GA]^AATT[CT]
AflIII       2010  ACGTGT               A^C[GA][CT]GT
AocI         1331  CCTCAGG              CC^T[ACGT]AGG
ApaI          779  GGGCCC               GGGCC^C
ApoI         1018  AAATTC               [GA]^AATT[CT]
AvrII        1651  CCTAGG               C^CTAGG
BamHI        1206  GGATCC               G^GATCC
BbsI          907  GAAGAC               GAAGAC(2/6)
BglII        2142  AGATCT               A^GATCT
BlnI         1651  CCTAGG               C^CTAGG
BpuAI         907  GAAGAC               GAAGAC(2/6)
BsaAI        1856  TACGTG               [CT]AC^GT[GA]
BsaWI         835  ACCGGA               [AT]^CCGG[AT]
Bsp1407I     1599  TGTACA               T^GTACA
BspHI          18  TCATGA               T^CATGA
BsrGI        1599  TGTACA               T^GTACA
BstUI        1778  CGCG                 CG^CG
Bsu36I       1331  CCTCAGG              CC^T[ACGT]AGG
DraI          455  TTTAAA               TTT^AAA
EagI         2754  CGGCCG               C^GGCCG
EclXI        2754  CGGCCG               C^GGCCG
Eco52I       2754  CGGCCG               C^GGCCG
Eco81I       1331  CCTCAGG              CC^T[ACGT]AGG
HincII        510  GTTAAC               GT[CT]^[GA]AC
HindII        510  GTTAAC               GT[CT]^[GA]AC
HpaI          510  GTTAAC               GTT^AAC
MslI           19  CATGAGAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI         1778  CGCG                 CG^CG
NruI         1777  TCGCGA               TCG^CGA
PpuMI        2019  AGGACCT              [GA]G^G[AT]CC[CT]
PvuI         1295  CGATCG               CGAT^CG
RcaI           18  TCATGA               T^CATGA
SacI         2504  GAGCTC               GAGCT^C
SbfI         2644  CCTGCAGG             CCTGCA^GG
ScaI         2685  AGTACT               AGT^ACT
SexAI        1605  ACCAGGT              A^CC[AT]GGT
SgrAI        2232  CGCCGGTG             C[GA]^CCGG[CT]G
SmaI          821  CCCGGG               CCC^GGG
SphI         1983  GCATGC               GCATG^C
Sse8387I     2644  CCTGCAGG             CCTGCA^GG
SspBI        1599  TGTACA               T^GTACA
XbaI          486  TCTAGA               T^CTAGA
XmaCI         821  CCCGGG               C^CCGGG
XmaI          821  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccI          419  GTCTAC               GT^[AC][GT]AC
             1511  GTAGAC              
Asp700I      1480  GAAGGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2317  GAAGCTGTTC          
BbeI         1196  GGCGCC               GGCGC^C
             2762  GGCGCC              
BcgI         1450  CGACCTGGGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             2551  CGAGCCCGCTGC        
BsaI         2051  GAGACC               GGTCTC(1/5)
              877  GGTCTC              
BsgI         1808  GTGCAG               GTGCAG(16/14)
             1943  GTGCAG              
BsiEI        1295  CGATCG               CG[GA][CT]^CG
             2754  CGGCCG              
BsrDI        1276  CATTGC               GCAATG(2/0)
             1641  GCAATG              
BssSI        1988  CACGAG               CACGAG(-5/-1)
             2477  CACGAG              
BstEII        573  GGTGACC              G^GT[ACGT]ACC
             1787  GGTGACC             
BstPI         573  GGTGACC              G^GT[ACGT]ACC
             1787  GGTGACC             
DraIII       1802  CACTGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2417  CACAAGGTG           
DrdI          916  GACGAACCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2053  GACCTTCGGGTC        
EcoO65I       573  GGTGACC              G^GT[ACGT]ACC
             1787  GGTGACC             
KasI         1196  GGCGCC               G^GCGCC
             2762  GGCGCC              
NaeI          768  GCCGGC               GCC^GGC
             1898  GCCGGC              
NarI         1196  GGCGCC               GG^CGCC
             2762  GGCGCC              
NgoMIV        768  GCCGGC               G^CCGGC
             1898  GCCGGC              
PflMI         545  CCAGAGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2416  CCACAAGGTGG         
PvuII        1257  CAGCTG               CAG^CTG
             2285  CAGCTG              
SfiI          746  GGCCCGAGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             2533  GGCCGAGGAGGCC       
SfoI         1196  GGCGCC               GGC^GCC
             2762  GGCGCC              
SmlI          150  CTTGAG               C^T[CT][GA]AG
             2387  CTCAAG              
TfiI          143  GAATC                G^A[AT]TC
              675  GAATC               
Van91I        545  CCAGAGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2416  CCACAAGGTGG         
XcmI         2081  CCACCACCCTGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2277  CCACCCTGCAGCTGG     
XmnI         1480  GAAGGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2317  GAAGCTGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  111 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        Alw44I      
AlwI        AlwNI       Aor51HI     ApaLI       AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbvI        BcnI        BfaI        BglI        BlpI        
BmyI        BpmI        Bpu1102I    BsaHI       BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrBI       BsrFI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       BstXI       BstYI       Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
Eco47III    EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PstI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StyI        TaqI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII