##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
Acc65I AccIII AflII Alw44I ApaLI
AscI AseI AsnI Asp700I Asp718I
AviII BbsI BclI BfrI BpuAI
BsaBI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BsrGI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DraI EagI EclXI
Eco52I EcoRV EcoT22I FbaI FspI
HincII HindII HpaI KpnI MamI
MfeI MluI MroI MseI MslI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PmeI PshBI
PvuI RcaI RsrII SalI SbfI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tru9I
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 3172 GACGTC GACGT^C
AccI 3975 GTCTAC GT^[AC][GT]AC
AclI 980 AACGTT AA^CGTT
AcsI 3644 GAATTC [GA]^AATT[CT]
AflIII 2959 ACATGT A^C[GA][CT]GT
AgeI 3288 ACCGGT A^CCGGT
AhdI 1117 GACTGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 3644 GAATTC [GA]^AATT[CT]
AspEI 1117 GACTGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2938 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1833 GGATCC G^GATCC
BbrPI 1400 CACGTG CAC^GTG
BcgI 1674 GCAGCTGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2577 GTATCC GTATCC(6/5)
BglII 811 AGATCT A^GATCT
BsaAI 1400 CACGTG [CT]AC^GT[GA]
BspLU11I 2959 ACATGT A^CATGT
BsrBI 1451 GAGCGG CCGCTC(-3/-3)
BsrDI 4102 CATTGC GCAATG(2/0)
BstEII 2555 GGTGACC G^GT[ACGT]ACC
BstPI 2555 GGTGACC G^GT[ACGT]ACC
Eam1105I 1117 GACTGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2555 GGTGACC G^GT[ACGT]ACC
EcoRI 3644 GAATTC G^AATTC
FseI 3342 GGCCGGCC GGCCGG^CC
HindIII 3856 AAGCTT A^AGCTT
PaeR7I 2016 CTCGAG C^TCGAG
PflFI 2938 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 264 CCAGGGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 3288 ACCGGT A^CCGGT
PmaCI 1400 CACGTG CAC^GTG
PmlI 1400 CACGTG CAC^GTG
PshAI 647 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 980 AACGTT AA^CGTT
SapI 3079 GCTCTTC GCTCTTC(1/4)
ScaI 588 AGTACT AGT^ACT
SexAI 3303 ACCTGGT A^CC[AT]GGT
SmlI 2016 CTCGAG C^T[CT][GA]AG
SphI 4012 GCATGC GCATG^C
Tth111I 2938 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 264 CCAGGGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 2016 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Aor51HI 876 AGCGCT AGC^GCT
3465 AGCGCT
AvrII 2087 CCTAGG C^CTAGG
3746 CCTAGG
BalI 342 TGGCCA TGG^CCA
1264 TGGCCA
BglI 496 GCCTGATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1257 GCCGAGATGGC
BlnI 2087 CCTAGG C^CTAGG
3746 CCTAGG
BsaI 891 GAGACC GGTCTC(1/5)
3943 GAGACC
BsaWI 2785 ACCGGA [AT]^CCGG[AT]
3288 ACCGGT
EarI 3080 CTCTTC CTCTTC(1/4)
3540 CTCTTC
Eco47III 876 AGCGCT AGC^GCT
3465 AGCGCT
Ksp632I 3080 CTCTTC CTCTTC(1/4)
3540 CTCTTC
KspI 87 CCGCGG CCGC^GG
1500 CCGCGG
MluNI 342 TGGCCA TGG^CCA
1264 TGGCCA
MscI 342 TGGCCA TGG^CCA
1264 TGGCCA
NaeI 1636 GCCGGC GCC^GGC
3343 GCCGGC
NcoI 694 CCATGG C^CATGG
3553 CCATGG
NgoMIV 1636 GCCGGC G^CCGGC
3343 GCCGGC
NspI 2959 ACATGT [GA]CATG^[CT]
4012 GCATGC
SacII 87 CCGCGG CCGC^GG
1500 CCGCGG
SmaI 1281 CCCGGG CCC^GGG
4065 CCCGGG
StuI 1665 AGGCCT AGG^CCT
2012 AGGCCT
XmaCI 1281 CCCGGG C^CCGGG
4065 CCCGGG
XmaI 1281 CCCGGG C^CCGGG
4065 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BanI BanII BbeI
BbvCI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DrdI DsaI EaeI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII