##################################
# NO CUT ENZYMES (   71 enzymes) #
##################################

AatII       AclI        AflII       AflIII      AgeI        
AhdI        AocI        AscI        AseI        AsnI        
Asp700I     AspEI       AviII       BamHI       BbrPI       
BcgI        BciVI       BfrI        BsaBI       BsiWI       
BspLU11I    BsrFI       BssHII      Bst1107I    BstBI       
BstZ17I     Bsu36I      Cfr10I      CpoI        DraI        
Eam1105I    Eco81I      EcoT22I     FseI        FspI        
HindIII     HpaI        KspI        MamI        MfeI        
MluI        MunI        NaeI        NdeI        NgoMIV      
NheI        NsiI        NspV        PacI        PinAI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PvuI        RsrII       SacI        SacII       SalI        
SapI        SbfI        SfiI        SfuI        SgrAI       
SnaBI       SpeI        Sse8387I    SspI        SwaI        
XmnI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

Acc65I        899  GGTACC               G^GTACC
AccI         2220  GTCTAC               GT^[AC][GT]AC
AccIII       3329  TCCGGA               T^CCGGA
Asp718I       899  GGTACC               G^GTACC
AspI         2462  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbeI         3231  GGCGCC               GGCGC^C
BclI         3198  TGATCA               T^GATCA
BglII         657  AGATCT               A^GATCT
BsaAI        3145  TACGTG               [CT]AC^GT[GA]
BseAI        3329  TCCGGA               T^CCGGA
BsiEI           2  CGGCCG               CG[GA][CT]^CG
BsmBI         434  CGTCTC               CGTCTC(1/5)
Bsp1407I      213  TGTACA               T^GTACA
BspDI         398  ATCGAT               AT^CGAT
BspEI        3329  TCCGGA               T^CCGGA
BspHI        2456  TCATGA               T^CATGA
BspMI         721  GCAGGT               ACCTGC(4/8)
BsrDI        3752  GCAATG               GCAATG(2/0)
BsrGI         213  TGTACA               T^GTACA
BssSI          71  CACGAG               CACGAG(-5/-1)
BstAPI       3042  GCAGAAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       2433  GGTGACC              G^GT[ACGT]ACC
BstPI        2433  GGTGACC              G^GT[ACGT]ACC
ClaI          398  ATCGAT               AT^CGAT
EagI            2  CGGCCG               C^GGCCG
EarI         3544  GAAGAG               CTCTTC(1/4)
EclXI           2  CGGCCG               C^GGCCG
Eco52I          2  CGGCCG               C^GGCCG
EcoO65I      2433  GGTGACC              G^GT[ACGT]ACC
EcoRI         307  GAATTC               G^AATTC
EcoRV         884  GATATC               GAT^ATC
FbaI         3198  TGATCA               T^GATCA
HincII        974  GTCAAC               GT[CT]^[GA]AC
HindII        974  GTCAAC               GT[CT]^[GA]AC
KasI         3231  GGCGCC               G^GCGCC
KpnI          899  GGTACC               GGTAC^C
Ksp632I      3544  GAAGAG               CTCTTC(1/4)
MroI         3329  TCCGGA               T^CCGGA
MseI          780  TTAA                 T^TAA
NarI         3231  GGCGCC               GG^CGCC
NotI            1  GCGGCCGC             GC^GGCCGC
NruI          535  TCGCGA               TCG^CGA
PaeR7I       3413  CTCGAG               C^TCGAG
PflFI        2462  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI        2307  GACCCCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI         2456  TCATGA               T^CATGA
ScaI         2784  AGTACT               AGT^ACT
SexAI         207  ACCTGGT              A^CC[AT]GGT
SfoI         3231  GGCGCC               GGC^GCC
SmlI         3413  CTCGAG               C^T[CT][GA]AG
SspBI         213  TGTACA               T^GTACA
Tru9I         780  TTAA                 T^TAA
Tth111I      2462  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         2025  TCTAGA               T^CTAGA
XcmI         3063  CCAGCCAGGGCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         3413  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AccII         133  CGCG                 CG^CG
              536  CGCG                
AcsI          307  GAATTC               [GA]^AATT[CT]
              767  GAATTT              
Alw44I        254  GTGCAC               G^TGCAC
             2898  GTGCAC              
AlwI         2209  GATCC                GGATC(4/5)
             2823  GATCC               
Aor51HI       681  AGCGCT               AGC^GCT
              999  AGCGCT              
ApaLI         254  GTGCAC               G^TGCAC
             2898  GTGCAC              
ApoI          307  GAATTC               [GA]^AATT[CT]
              767  GAATTT              
AvrII        1181  CCTAGG               C^CTAGG
             3435  CCTAGG              
BlnI         1181  CCTAGG               C^CTAGG
             3435  CCTAGG              
BsaWI        1027  ACCGGA               [AT]^CCGG[AT]
             3329  TCCGGA              
BsmI         2274  GCATTC               GAATGC(1/-1)
             3631  GCATTC              
BstUI         133  CGCG                 CG^CG
              536  CGCG                
BstXI         807  CCAACCATGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1916  CCAGTGGTATGG        
DraIII       2624  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2646  CACGGGGTG           
DrdI          338  GACAAGCAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1099  GACAGCATGGTC        
Eco47III      681  AGCGCT               AGC^GCT
              999  AGCGCT              
MaeII        3146  ACGT                 A^CGT
             3404  ACGT                
MslI         1331  CACATCAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2499  CATCACTGTG          
MvnI          133  CGCG                 CG^CG
              536  CGCG                
SphI         1303  GCATGC               GCATG^C
             2511  GCATGC              


#########################################
# 3 OR MORE CUT ENZYMES (  111 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AlwNI       
ApaI        AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbsI        BbvCI       BbvI        
BcnI        BfaI        BglI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaHI       BsaI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BsrBI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       CelII       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        MaeI        MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MspA1I      MspI        MvaI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        PpuMI       PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmaI        StuI        StyI        
TaqI        TfiI        TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XhoII       XmaCI       
XmaI