##################################
# NO CUT ENZYMES (   56 enzymes) #
##################################

Acc65I      AflIII      Aor51HI     AscI        AseI        
AsnI        Asp718I     AviII       BamHI       BglII       
BsiWI       BsmBI       BsmI        BspLU11I    BssHII      
BstBI       CpoI        Eco47III    EcoRV       EcoT22I     
FseI        FspI        HincII      HindII      HpaI        
KpnI        KspI        MfeI        MluI        MunI        
NaeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PmeI        
PshAI       PshBI       PvuI        RsrII       SacII       
SalI        SbfI        SfiI        SfuI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SwaI        
XhoI        


###################################
# ONE CUT ENZYMES (   60 enzymes) #
###################################

AatII        2370  GACGTC               GACGT^C
AccII        2661  CGCG                 CG^CG
AccIII       1577  TCCGGA               T^CCGGA
AclI          329  AACGTT               AA^CGTT
AgeI         1571  ACCGGT               A^CCGGT
AhdI          130  GACTTCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1510  GTGCAC               G^TGCAC
ApaLI        1510  GTGCAC               G^TGCAC
AspEI         130  GACTTCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1327  GACCTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        3444  CCTAGG               C^CTAGG
BbeI           62  GGCGCC               GGCGC^C
BbrPI        3302  CACGTG               CAC^GTG
BbvCI        1845  CCTCAGC              CCTCAGC(-5/-2)
BglI         1741  GCCCTGAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI         3444  CCTAGG               C^CTAGG
BlpI         2265  GCTTAGC              GC^T[ACGT]AGC
Bpu1102I     2265  GCTTAGC              GC^T[ACGT]AGC
BsaAI        3302  CACGTG               [CT]AC^GT[GA]
BsaBI        1075  GATCAGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI        1577  TCCGGA               T^CCGGA
BsiEI          95  CGGCCG               CG[GA][CT]^CG
Bsp1407I      905  TGTACA               T^GTACA
BspDI        1246  ATCGAT               AT^CGAT
BspEI        1577  TCCGGA               T^CCGGA
BsrGI         905  TGTACA               T^GTACA
BssSI        2816  CTCGTG               CACGAG(-5/-1)
Bst1107I     1953  GTATAC               GTA^TAC
BstUI        2661  CGCG                 CG^CG
BstZ17I      1953  GTATAC               GTA^TAC
CelII        2265  GCTTAGC              GC^T[ACGT]AGC
ClaI         1246  ATCGAT               AT^CGAT
EagI           95  CGGCCG               C^GGCCG
Eam1105I      130  GACTTCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI          95  CGGCCG               C^GGCCG
Eco52I         95  CGGCCG               C^GGCCG
EcoRI        3436  GAATTC               G^AATTC
HindIII      1400  AAGCTT               A^AGCTT
KasI           62  GGCGCC               G^GCGCC
MamI         1075  GATCAGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         1577  TCCGGA               T^CCGGA
MvnI         2661  CGCG                 CG^CG
NarI           62  GGCGCC               GG^CGCC
NcoI         3292  CCATGG               C^CATGG
NdeI         3172  CATATG               CA^TATG
NspI         2166  GCATGT               [GA]CATG^[CT]
PflFI        1327  GACCTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        1571  ACCGGT               A^CCGGT
PmaCI        3302  CACGTG               CAC^GTG
PmlI         3302  CACGTG               CAC^GTG
Psp1406I      329  AACGTT               AA^CGTT
SapI         3361  GCTCTTC              GCTCTTC(1/4)
ScaI         2111  AGTACT               AGT^ACT
SexAI         789  ACCAGGT              A^CC[AT]GGT
SfoI           62  GGCGCC               GGC^GCC
SspBI         905  TGTACA               T^GTACA
SspI         3921  AATATT               AAT^ATT
Tth111I      1327  GACCTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         4262  TCTAGA               T^CTAGA
XcmI         3298  CCACCACGTGGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AccI         1006  GTAGAC               GT^[AC][GT]AC
             1953  GTATAC              
AflII        1306  CTTAAG               C^TTAAG
             3479  CTTAAG              
BcgI         3197  CGAGGCTGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             3711  CGAAGCATCTGC        
BciVI        2139  GGATAC               GTATCC(6/5)
              113  GTATCC              
BclI         1074  TGATCA               T^GATCA
             1533  TGATCA              
BfrI         1306  CTTAAG               C^TTAAG
             3479  CTTAAG              
BsrBI         721  GAGCGG               CCGCTC(-3/-3)
             2464  GAGCGG              
BsrDI        2574  CATTGC               GCAATG(2/0)
             3783  CATTGC              
BstAPI       1961  GCAAGGCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2044  GCAAAATATGC         
BstEII       2869  GGTGACC              G^GT[ACGT]ACC
             3521  GGTCACC             
BstPI        2869  GGTGACC              G^GT[ACGT]ACC
             3521  GGTCACC             
BstXI        3286  CCAGGTCCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3301  CCACGTGGCTGG        
EcoNI         762  CCTCTGCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2324  CCTACTGGAGG         
EcoO65I      2869  GGTGACC              G^GT[ACGT]ACC
             3521  GGTCACC             
FbaI         1074  TGATCA               T^GATCA
             1533  TGATCA              
HgaI           80  GACGC                GACGC(5/10)
             2659  GACGC               
SacI          688  GAGCTC               GAGCT^C
             1426  GAGCTC              
SmaI          490  CCCGGG               CCC^GGG
             1722  CCCGGG              
StuI         1964  AGGCCT               AGG^CCT
             2668  AGGCCT              
XmaCI         490  CCCGGG               C^CCGGG
             1722  CCCGGG              
XmaI          490  CCCGGG               C^CCGGG
             1722  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  121 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AluI        
AlwI        AlwNI       AocI        ApaI        ApoI        
Asp700I     AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbsI        BbvI        BcnI        
BfaI        BmyI        BpmI        BpuAI       BsaHI       
BsaI        BsaJI       BsaWI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspHI       BspMI       BsrFI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
Bsu36I      Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraI        DraII       DraIII      
DrdI        DsaI        EaeI        EarI        Eco81I      
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PflMI       PleI        PpuMI       PstI        
PvuII       RcaI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XhoII       
XmnI