##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AccIII AclI AgeI Alw44I Aor51HI
ApaLI AscI AspI AvrII BamHI
BbrPI BciVI BclI BglI BlnI
BsaBI BsaWI BseAI BsiWI BsmBI
BspDI BspEI BspLU11I BssHII BstAPI
BstBI ClaI CpoI DraIII Eco47III
EcoNI FbaI FseI HpaI MamI
MfeI MluI MroI MunI NheI
NotI NruI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I Tth111I
Van91I
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Acc65I 2015 GGTACC G^GTACC
AflII 4196 CTTAAG C^TTAAG
AflIII 1186 ACGTGT A^C[GA][CT]GT
AhdI 4017 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 4275 GGATC GGATC(4/5)
ApaI 729 GGGCCC GGGCC^C
AseI 2737 ATTAAT AT^TAAT
AsnI 2737 ATTAAT AT^TAAT
Asp718I 2015 GGTACC G^GTACC
AspEI 4017 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1657 TGCGCA TGC^GCA
BbeI 4369 GGCGCC GGCGC^C
BbvCI 4397 GCTGAGG CCTCAGC(-5/-2)
BcgI 694 GCACATCTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 4196 CTTAAG C^TTAAG
BglII 2838 AGATCT A^GATCT
BsaAI 342 TACGTG [CT]AC^GT[GA]
BsiEI 1643 CGGCCG CG[GA][CT]^CG
BsrBI 578 GAGCGG CCGCTC(-3/-3)
BsrDI 1116 CATTGC GCAATG(2/0)
BssSI 1604 CACGAG CACGAG(-5/-1)
Bst1107I 3879 GTATAC GTA^TAC
BstEII 1847 GGTCACC G^GT[ACGT]ACC
BstPI 1847 GGTCACC G^GT[ACGT]ACC
BstYI 2838 AGATCT [GA]^GATC[CT]
BstZ17I 3879 GTATAC GTA^TAC
DrdI 599 GACTGAAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1643 CGGCCG C^GGCCG
Eam1105I 4017 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1643 CGGCCG C^GGCCG
Eco52I 1643 CGGCCG C^GGCCG
EcoO65I 1847 GGTCACC G^GT[ACGT]ACC
EcoRI 3020 GAATTC G^AATTC
EcoRV 86 GATATC GAT^ATC
FspI 1657 TGCGCA TGC^GCA
KasI 4369 GGCGCC G^GCGCC
KpnI 2015 GGTACC GGTAC^C
MflI 2838 AGATCT [GA]^GATC[CT]
NaeI 1675 GCCGGC GCC^GGC
NarI 4369 GGCGCC GG^CGCC
NdeI 4155 CATATG CA^TATG
NgoMIV 1675 GCCGGC G^CCGGC
NspI 4112 ACATGC [GA]CATG^[CT]
PaeR7I 211 CTCGAG C^TCGAG
PshBI 2737 ATTAAT AT^TAAT
SacI 1484 GAGCTC GAGCT^C
SfoI 4369 GGCGCC GGC^GCC
SmaI 1577 CCCGGG CCC^GGG
SwaI 3457 ATTTAAAT ATTT^AAAT
XhoI 211 CTCGAG C^TCGAG
XhoII 2838 AGATCT [GA]^GATC[CT]
XmaCI 1577 CCCGGG C^CCGGG
XmaI 1577 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AatII 740 GACGTC GACGT^C
1949 GACGTC
AlwNI 438 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
473 CAGCAGCTG
BalI 2684 TGGCCA TGG^CCA
2701 TGGCCA
BlpI 533 GCTGAGC GC^T[ACGT]AGC
689 GCTGAGC
Bpu1102I 533 GCTGAGC GC^T[ACGT]AGC
689 GCTGAGC
BsgI 444 CTGCAC GTGCAG(16/14)
4460 CTGCAC
Bsp1407I 814 TGTACA T^GTACA
2484 TGTACA
BspHI 370 TCATGA T^CATGA
3145 TCATGA
BsrFI 1675 GCCGGC [GA]^CCGG[CT]
1936 GCCGGT
BsrGI 814 TGTACA T^GTACA
2484 TGTACA
BstXI 4248 CCAGCACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4382 CCAGCTACTTGG
CelII 533 GCTGAGC GC^T[ACGT]AGC
689 GCTGAGC
Cfr10I 1675 GCCGGC [GA]^CCGG[CT]
1936 GCCGGT
EcoT22I 2824 ATGCAT ATGCA^T
4114 ATGCAT
HaeII 1678 GGCGCT [GA]GCGC^[CT]
4369 GGCGCC
HindIII 1167 AAGCTT A^AGCTT
3631 AAGCTT
MluNI 2684 TGGCCA TGG^CCA
2701 TGGCCA
MscI 2684 TGGCCA TGG^CCA
2701 TGGCCA
NcoI 1361 CCATGG C^CATGG
1778 CCATGG
NsiI 2824 ATGCAT ATGCA^T
4114 ATGCAT
PshAI 14 GACACAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1516 GACCCGTGTC
RcaI 370 TCATGA T^CATGA
3145 TCATGA
SalI 1855 GTCGAC G^TCGAC
1946 GTCGAC
SfaNI 1867 GATGC GCATC(5/9)
2151 GATGC
SspBI 814 TGTACA T^GTACA
2484 TGTACA
XbaI 2712 TCTAGA T^CTAGA
4128 TCTAGA
XcmI 1352 CCAGTCTCCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1603 CCACGAGCCCGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AocI ApoI Asp700I
AspHI AvaI AvaII BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PstI PvuII RsaI SacII
Sau3AI Sau96I ScrFI SfcI SmlI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XmnI