##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
Acc65I AccI AclI AcsI AgeI
AhdI Alw44I ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BamHI BbeI BbrPI BlnI
BsaAI BsiEI BsiWI Bsp1407I BspDI
BsrGI BssHII BssSI Bst1107I BstBI
BstXI BstZ17I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco52I
EcoRI EcoRV FseI FspI HindIII
HpaI KasI KpnI KspI MfeI
MluI MunI NarI NotI NruI
NspV PacI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacII SalI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SspBI SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccII 3966 CGCG CG^CG
AccIII 1942 TCCGGA T^CCGGA
AflII 773 CTTAAG C^TTAAG
Aor51HI 2907 AGCGCT AGC^GCT
ApaI 1089 GGGCCC GGGCC^C
Asp700I 923 GAACGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 3820 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 148 GGCACC G^G[CT][GA]CC
BcgI 2878 CGAGGAACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2937 TGATCA T^GATCA
BfrI 773 CTTAAG C^TTAAG
BglII 1132 AGATCT A^GATCT
BsaBI 946 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1942 TCCGGA T^CCGGA
BspEI 1942 TCCGGA T^CCGGA
BspHI 1578 TCATGA T^CATGA
BstEII 3361 GGTGACC G^GT[ACGT]ACC
BstPI 3361 GGTGACC G^GT[ACGT]ACC
BstUI 3966 CGCG CG^CG
DraIII 4013 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 3102 CCATGG C^C[GA][CT]GG
Eco47III 2907 AGCGCT AGC^GCT
EcoO65I 3361 GGTGACC G^GT[ACGT]ACC
EcoT22I 1295 ATGCAT ATGCA^T
FbaI 2937 TGATCA T^GATCA
HincII 500 GTCAAC GT[CT]^[GA]AC
HindII 500 GTCAAC GT[CT]^[GA]AC
MamI 946 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1942 TCCGGA T^CCGGA
MvnI 3966 CGCG CG^CG
NaeI 1626 GCCGGC GCC^GGC
NcoI 3102 CCATGG C^CATGG
NdeI 4035 CATATG CA^TATG
NgoMIV 1626 GCCGGC G^CCGGC
NsiI 1295 ATGCAT ATGCA^T
PaeR7I 180 CTCGAG C^TCGAG
PflFI 3820 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2144 CCAAAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 2444 GACGCCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1578 TCATGA T^CATGA
SphI 2226 GCATGC GCATG^C
Tth111I 3820 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2144 CCAAAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 180 CTCGAG C^TCGAG
XmnI 923 GAACGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AatII 1198 GACGTC GACGT^C
3820 GACGTC
AocI 1744 CCTCAGG CC^T[ACGT]AGG
3572 CCTGAGG
BbvCI 3995 CCTCAGC CCTCAGC(-5/-2)
4059 CCTCAGC
BciVI 1750 GGATAC GTATCC(6/5)
3379 GGATAC
BsaWI 998 TCCGGT [AT]^CCGG[AT]
1942 TCCGGA
BsmBI 1793 GAGACG CGTCTC(1/5)
3818 GAGACG
BsrDI 1776 GCAATG GCAATG(2/0)
2628 GCAATG
BsrFI 1626 GCCGGC [GA]^CCGG[CT]
2447 GCCGGT
Bsu36I 1744 CCTCAGG CC^T[ACGT]AGG
3572 CCTGAGG
Cfr10I 1626 GCCGGC [GA]^CCGG[CT]
2447 GCCGGT
Eco81I 1744 CCTCAGG CC^T[ACGT]AGG
3572 CCTGAGG
HaeII 665 AGCGCC [GA]GCGC^[CT]
2907 AGCGCT
NheI 658 GCTAGC G^CTAGC
3179 GCTAGC
SmaI 591 CCCGGG CCC^GGG
3300 CCCGGG
TaqI 181 TCGA T^CGA
2386 TCGA
TthHB8I 181 TCGA T^CGA
2386 TCGA
XcmI 2400 CCAAGCTGCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3405 CCAGAAAGAGTCTGG
XmaCI 591 CCCGGG C^CCGGG
3300 CCCGGG
XmaI 591 CCCGGG C^CCGGG
3300 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AciI AcyI AfaI AflIII AluI
AlwI AlwNI AspHI AvaI AvaII
BalI BanII BbsI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspLU11I BspMI BsrBI BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
SbfI ScrFI SfaNI SfcI SmlI
Sse8387I StuI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII