##################################
# NO CUT ENZYMES (  130 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AgeI        Alw44I      AocI        
Aor51HI     ApaLI       AscI        AseI        AsnI        
Asp718I     AspI        AvaI        AviII       BalI        
BamHI       BanI        BbeI        BbrPI       BcgI        
BciVI       BclI        BcnI        BfrI        BglII       
BlpI        Bpu1102I    BsaBI       BsaHI       BsaWI       
BseAI       BsgI        BsiEI       BsiWI       BsmBI       
BsoBI       Bsp1407I    BspDI       BspEI       BspMI       
BsrBI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstEII      BstPI       BstUI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DrdI        EaeI        EagI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     FbaI        
FseI        FspI        HapII       Hin1I       HindIII     
HpaI        HpaII       KasI        KpnI        KspI        
MamI        MfeI        MluI        MluNI       MroI        
MscI        MspI        MunI        MvnI        NaeI        
NarI        NciI        NdeI        NgoMIV      NheI        
NotI        NruI        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshBI       Psp1406I    PvuI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SgrAI       SmaI        
SnaBI       Sse8387I    SspBI       SwaI        Tth111I     
Van91I      XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccI          437  GTAGAC               GT^[AC][GT]AC
AflIII       1474  ACATGT               A^C[GA][CT]GT
AhdI          872  GACTTTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI         474  CAGTGACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          497  GGGCCC               GGGCC^C
AspEI         872  GACTTTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI          12  GTGCTC               G[AT]GC[AT]^C
AvrII        1669  CCTAGG               C^CTAGG
BglI         1295  GCCAATCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI         1669  CCTAGG               C^CTAGG
BsaAI          39  CACGTA               [CT]AC^GT[GA]
BsaI          778  GGTCTC               GGTCTC(1/5)
BsiHKAI        12  GTGCTC               G[AT]GC[AT]^C
BspLU11I     1474  ACATGT               A^CATGT
BsrDI         222  GCAATG               GCAATG(2/0)
BstAPI       1909  GCACTTTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        1092  TTCGAA               TT^CGAA
BstXI        1192  CCAAGCTGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         969  GGATCT               [GA]^GATC[CT]
DraI         2442  TTTAAA               TTT^AAA
DraII        1540  GGGACCT              [GA]G^G[ACGT]CC[CT]
DraIII       1910  CACTTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI         2643  CCATGG               C^C[GA][CT]GG
Eam1105I      872  GACTTTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I     1540  GGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRI        2451  GAATTC               G^AATTC
EcoT22I      2268  ATGCAT               ATGCA^T
FauI          495  GCGGG                CCCGC(4/6)
HgaI         1381  GCGTC                GACGC(5/10)
HincII       1383  GTCAAC               GT[CT]^[GA]AC
HindII       1383  GTCAAC               GT[CT]^[GA]AC
MaeII          40  ACGT                 A^CGT
MflI          969  GGATCT               [GA]^GATC[CT]
NcoI         2643  CCATGG               C^CATGG
NsiI         2268  ATGCAT               ATGCA^T
NspV         1092  TTCGAA               TT^CGAA
PpuMI        1540  GGGACCT              [GA]G^G[AT]CC[CT]
PshAI         800  GACCGCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfuI         1092  TTCGAA               TT^CGAA
SpeI         2284  ACTAGT               A^CTAGT
SphI           34  GCATGC               GCATG^C
StuI          958  AGGCCT               AGG^CCT
XbaI         2661  TCTAGA               T^CTAGA
XhoII         969  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AlwI          898  GATCC                GGATC(4/5)
              969  GGATC               
BanII         497  GGGCCC               G[GA]GC[CT]^C
             2498  GAGCCC              
BbsI          331  GAAGAC               GAAGAC(2/6)
             1166  GAAGAC              
BbvCI        1039  CCTCAGC              CCTCAGC(-5/-2)
               80  GCTGAGG             
BpuAI         331  GAAGAC               GAAGAC(2/6)
             1166  GAAGAC              
BsiYI         485  CCCAATGATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1634  CCTAGATTTGG         
BslI          485  CCCAATGATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1634  CCTAGATTTGG         
BspHI        1242  TCATGA               T^CATGA
             1440  TCATGA              
EcoRV         278  GATATC               GAT^ATC
             1055  GATATC              
HaeII         363  GGCGCT               [GA]GCGC^[CT]
             1401  AGCGCC              
NspI           34  GCATGC               [GA]CATG^[CT]
             1474  ACATGT              
PstI          432  CTGCAG               CTGCA^G
             1781  CTGCAG              
PvuII         116  CAGCTG               CAG^CTG
              608  CAGCTG              
RcaI         1242  TCATGA               T^CATGA
             1440  TCATGA              
SspI         2579  AATATT               AAT^ATT
             2782  AATATT              


#########################################
# 3 OR MORE CUT ENZYMES (   69 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
Asp700I     AvaII       BbvI        BfaI        BmyI        
BpmI        BsaJI       BseRI       BsmAI       BsmFI       
BsmI        Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       EarI        EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HhaI        HinP1I      HinfI       
HphI        ItaI        Ksp632I     MaeI        MaeIII      
MboI        MboII       MnlI        MseI        MslI        
MspA1I      MvaI        MwoI        NdeII       NlaIII      
NlaIV       PleI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XmnI