##################################
# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaII AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BlnI BsaAI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BspEI
BspLU11I BspMI BsrBI BsrFI BssHII
BssSI BstBI BstUI BstXI Bsu36I
Cfr10I CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoT14I FauI FbaI FseI
FspI HaeII HincII HindII HpaI
KasI KpnI KspI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NotI NruI NspI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I StyI SwaI Tth111I Van91I
XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AccI 1919 GTATAC GT^[AC][GT]AC
AciI 140 CCGC CCGC(-3/-1)
AcyI 19 GGCGTC G[GA]^CG[CT]C
ApaI 257 GGGCCC GGGCC^C
Asp700I 1422 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 6 CTCGAG C^[CT]CG[GA]G
BanI 11 GGTGCC G^G[CT][GA]CC
BcnI 317 CCGGG CC^[GC]GG
BglII 1424 AGATCT A^GATCT
BlpI 1583 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 1583 GCTTAGC GC^T[ACGT]AGC
BsaHI 19 GGCGTC G[GA]^CG[CT]C
BsaJI 15 CCACGG C^C[ACGT][ACGT]GG
BsiYI 140 CCGCACTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 140 CCGCACTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 1640 GGGAC GGGAC(10/14)
BsmI 905 GCATTC GAATGC(1/-1)
BsoBI 6 CTCGAG C^[CT]CG[GA]G
Bsp1407I 931 TGTACA T^GTACA
BspDI 519 ATCGAT AT^CGAT
BspHI 1544 TCATGA T^CATGA
BsrGI 931 TGTACA T^GTACA
Bst1107I 1919 GTATAC GTA^TAC
BstEII 863 GGTTACC G^GT[ACGT]ACC
BstPI 863 GGTTACC G^GT[ACGT]ACC
BstZ17I 1919 GTATAC GTA^TAC
CelII 1583 GCTTAGC GC^T[ACGT]AGC
CfoI 1266 GCGC GCG^C
ClaI 519 ATCGAT AT^CGAT
DraI 590 TTTAAA TTT^AAA
DsaI 15 CCACGG C^C[GA][CT]GG
EcoO65I 863 GGTTACC G^GT[ACGT]ACC
EcoRI 1695 GAATTC G^AATTC
EcoRV 1056 GATATC GAT^ATC
EcoT22I 1822 ATGCAT ATGCA^T
HapII 317 CCGG C^CGG
HgaI 20 GCGTC GACGC(5/10)
HhaI 1266 GCGC GCG^C
Hin1I 19 GGCGTC G[GA]^CG[CT]C
HinP1I 1266 GCGC G^CGC
HindIII 1581 AAGCTT A^AGCTT
HpaII 317 CCGG C^CGG
MaeII 407 ACGT A^CGT
MspI 317 CCGG C^CGG
NciI 317 CCGGG CC^[GC]GG
NheI 995 GCTAGC G^CTAGC
NsiI 1822 ATGCAT ATGCA^T
PaeR7I 6 CTCGAG C^TCGAG
RcaI 1544 TCATGA T^CATGA
SapI 1560 GCTCTTC GCTCTTC(1/4)
SexAI 326 ACCTGGT A^CC[AT]GGT
SspBI 931 TGTACA T^GTACA
SspI 2082 AATATT AAT^ATT
StuI 1085 AGGCCT AGG^CCT
Tsp45I 1955 GTCAC ^GT[GC]AC
TspRI 1722 CACTG CA[GC]TG[ACGT][ACGT]^
XhoI 6 CTCGAG C^TCGAG
XmnI 1422 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcsI 1695 GAATTC [GA]^AATT[CT]
1741 AAATTT
AlwI 320 GGATC GGATC(4/5)
428 GGATC
AlwNI 1638 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
1935 CAGCTACTG
ApoI 1695 GAATTC [GA]^AATT[CT]
1741 AAATTT
BanII 3 GGGCTC G[GA]GC[CT]^C
257 GGGCCC
BbsI 335 GAAGAC GAAGAC(2/6)
1608 GAAGAC
BmyI 3 GGGCTC G[AGT]GC[ACT]^C
257 GGGCCC
BpuAI 335 GAAGAC GAAGAC(2/6)
1608 GAAGAC
Bsp1286I 3 GGGCTC G[AGT]GC[ACT]^C
257 GGGCCC
BstAPI 1409 GCAGACTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1934 GCAGCTACTGC
DraII 256 GGGGCCC [GA]G^G[ACGT]CC[CT]
257 GGGCCCT
EaeI 137 TGGCCG [CT]^GGCC[GA]
394 TGGCCG
EarI 1561 CTCTTC CTCTTC(1/4)
756 GAAGAG
EcoO109I 256 GGGGCCC [GA]G^G[ACGT]CC[CT]
257 GGGCCCT
HphI 2033 GGTGA GGTGA(8/7)
1788 TCACC
Ksp632I 1561 CTCTTC CTCTTC(1/4)
756 GAAGAG
MslI 1124 CATAGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1944 CATCTTCATG
PstI 1219 CTGCAG CTGCA^G
1332 CTGCAG
SmlI 6 CTCGAG C^T[CT][GA]AG
434 CTTGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AfaI AluI BbvI BfaI BpmI
BsaBI BsmAI BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII HinfI ItaI MaeI MaeIII
MamI MboI MboII MflI MnlI
MseI MspA1I MvaI MwoI NdeII
NlaIII NlaIV PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TfiI Tru9I TseI Tsp509I
TthHB8I XcmI XhoII