##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AgeI Aor51HI AscI AseI AsnI
AspI AviII BbrPI BbvCI BcgI
BsaBI BsrBI BssHII Bst1107I BstZ17I
CpoI DrdI EagI EclXI Eco47III
Eco52I EcoRV EcoT22I FseI FspI
HpaI KspI MamI MfeI MluI
MunI NheI NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshBI PvuI RsrII
SacII SalI SbfI SexAI SfiI
SgrAI SnaBI SphI Sse8387I SspI
StuI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 1703 GGTACC G^GTACC
AccI 4684 GTCTAC GT^[AC][GT]AC
AccIII 85 TCCGGA T^CCGGA
AclI 152 AACGTT AA^CGTT
AhdI 315 GACCCTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1349 CCTCAGG CC^T[ACGT]AGG
Asp718I 1703 GGTACC G^GTACC
AspEI 315 GACCCTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 122 GGATCC G^GATCC
BciVI 1861 GGATAC GTATCC(6/5)
BclI 2369 TGATCA T^GATCA
BglI 4380 GCCTTTATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 839 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 839 GCTCAGC GC^T[ACGT]AGC
BsaAI 1866 CACGTA [CT]AC^GT[GA]
BseAI 85 TCCGGA T^CCGGA
BsiEI 1012 CGGTCG CG[GA][CT]^CG
BsiWI 2053 CGTACG C^GTACG
Bsp1407I 5193 TGTACA T^GTACA
BspDI 3947 ATCGAT AT^CGAT
BspEI 85 TCCGGA T^CCGGA
BsrGI 5193 TGTACA T^GTACA
BssSI 726 CTCGTG CACGAG(-5/-1)
BstBI 1343 TTCGAA TT^CGAA
Bsu36I 1349 CCTCAGG CC^T[ACGT]AGG
CelII 839 GCTCAGC GC^T[ACGT]AGC
ClaI 3947 ATCGAT AT^CGAT
Eam1105I 315 GACCCTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1349 CCTCAGG CC^T[ACGT]AGG
FbaI 2369 TGATCA T^GATCA
KpnI 1703 GGTACC GGTAC^C
MroI 85 TCCGGA T^CCGGA
NaeI 3790 GCCGGC GCC^GGC
NgoMIV 3790 GCCGGC G^CCGGC
NspV 1343 TTCGAA TT^CGAA
Psp1406I 152 AACGTT AA^CGTT
SapI 3353 GCTCTTC GCTCTTC(1/4)
SfuI 1343 TTCGAA TT^CGAA
SpeI 2641 ACTAGT A^CTAGT
SspBI 5193 TGTACA T^GTACA
XbaI 4163 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AatII 1435 GACGTC GACGT^C
1946 GACGTC
AccII 1562 CGCG CG^CG
4555 CGCG
AflII 1808 CTTAAG C^TTAAG
4183 CTTAAG
Alw44I 4780 GTGCAC G^TGCAC
4969 GTGCAC
AlwNI 3398 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
3763 CAGCCTCTG
ApaI 3709 GGGCCC GGGCC^C
4906 GGGCCC
ApaLI 4780 GTGCAC G^TGCAC
4969 GTGCAC
AvrII 3408 CCTAGG C^CTAGG
3546 CCTAGG
BbeI 196 GGCGCC GGCGC^C
4193 GGCGCC
BfrI 1808 CTTAAG C^TTAAG
4183 CTTAAG
BglII 464 AGATCT A^GATCT
4579 AGATCT
BlnI 3408 CCTAGG C^CTAGG
3546 CCTAGG
BsgI 3659 GTGCAG GTGCAG(16/14)
3723 GTGCAG
BsmI 4867 GAATGC GAATGC(1/-1)
302 GCATTC
BspLU11I 2711 ACATGT A^CATGT
3866 ACATGT
BsrDI 4134 CATTGC GCAATG(2/0)
4679 GCAATG
BstAPI 302 GCATTCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
864 GCAAAAGCTGC
BstEII 998 GGTAACC G^GT[ACGT]ACC
2084 GGTTACC
BstPI 998 GGTAACC G^GT[ACGT]ACC
2084 GGTTACC
BstUI 1562 CGCG CG^CG
4555 CGCG
BstXI 1624 CCAACGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2074 CCAAGCAGCTGG
DraIII 230 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
4022 CACCGTGTG
EcoNI 561 CCTGAAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3403 CCTGGCCTAGG
EcoO65I 998 GGTAACC G^GT[ACGT]ACC
2084 GGTTACC
EcoRI 384 GAATTC G^AATTC
4942 GAATTC
HincII 942 GTTGAC GT[CT]^[GA]AC
2352 GTCAAC
HindII 942 GTTGAC GT[CT]^[GA]AC
2352 GTCAAC
HindIII 1317 AAGCTT A^AGCTT
3924 AAGCTT
KasI 196 GGCGCC G^GCGCC
4193 GGCGCC
MvnI 1562 CGCG CG^CG
4555 CGCG
NarI 196 GGCGCC GG^CGCC
4193 GGCGCC
NcoI 922 CCATGG C^CATGG
2782 CCATGG
PflMI 707 CCATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1689 CCAGGGGATGG
PpuMI 1996 GGGACCC [GA]G^G[AT]CC[CT]
4358 AGGACCT
ScaI 1042 AGTACT AGT^ACT
3190 AGTACT
SfoI 196 GGCGCC GGC^GCC
4193 GGCGCC
SmaI 1447 CCCGGG CCC^GGG
4151 CCCGGG
Van91I 707 CCATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1689 CCAGGGGATGG
XcmI 1909 CCAAGTCTGATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4131 CCACATTGCTGTTGG
XmaCI 1447 CCCGGG C^CCGGG
4151 CCCGGG
XmaI 1447 CCCGGG C^CCGGG
4151 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI ApoI Asp700I AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BsaWI BseRI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspHI BspMI BsrFI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeI NdeII
NlaIII NlaIV NspI PleI PshAI
PstI PvuII RcaI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI