##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AgeI AhdI AscI Asp718I AspEI
AviII AvrII BamHI BbrPI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BspLU11I BsrFI BsrGI BssHII
Bst1107I BstBI BstZ17I CelII Cfr10I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco52I EcoRI EcoT22I FseI
FspI HpaI KpnI MamI MfeI
MluI MroI MunI NaeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacI ScaI SfiI SfuI SgrAI
SnaBI SpeI SspBI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 3757 GTCGAC GT^[AC][GT]AC
AflII 2748 CTTAAG C^TTAAG
Alw44I 1033 GTGCAC G^TGCAC
AlwNI 276 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 3174 CCTCAGG CC^T[ACGT]AGG
Aor51HI 3367 AGCGCT AGC^GCT
ApaI 39 GGGCCC GGGCC^C
ApaLI 1033 GTGCAC G^TGCAC
AvaI 36 CCCGGG C^[CT]CG[GA]G
BbeI 3723 GGCGCC GGCGC^C
BcgI 3749 GCAAGATTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 97 GGATAC GTATCC(6/5)
BclI 1788 TGATCA T^GATCA
BfrI 2748 CTTAAG C^TTAAG
BglII 4419 AGATCT A^GATCT
BsaI 162 GAGACC GGTCTC(1/5)
BsmBI 1896 CGTCTC CGTCTC(1/5)
BsoBI 36 CCCGGG C^[CT]CG[GA]G
BspHI 3552 TCATGA T^CATGA
BssSI 1602 CACGAG CACGAG(-5/-1)
BstEII 1907 GGTCACC G^GT[ACGT]ACC
BstPI 1907 GGTCACC G^GT[ACGT]ACC
BstXI 873 CCATATCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 3174 CCTCAGG CC^T[ACGT]AGG
Eco47III 3367 AGCGCT AGC^GCT
Eco81I 3174 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1907 GGTCACC G^GT[ACGT]ACC
EcoRV 4468 GATATC GAT^ATC
FbaI 1788 TGATCA T^GATCA
KasI 3723 GGCGCC G^GCGCC
KspI 63 CCGCGG CCGC^GG
NarI 3723 GGCGCC GG^CGCC
NcoI 1047 CCATGG C^CATGG
NdeI 2228 CATATG CA^TATG
PpuMI 3102 AGGTCCT [GA]G^G[AT]CC[CT]
RcaI 3552 TCATGA T^CATGA
SacII 63 CCGCGG CCGC^GG
SalI 3757 GTCGAC G^TCGAC
SapI 482 GAAGAGC GCTCTTC(1/4)
SbfI 1865 CCTGCAGG CCTGCA^GG
SexAI 1057 ACCAGGT A^CC[AT]GGT
SfoI 3723 GGCGCC GGC^GCC
SmaI 36 CCCGGG CCC^GGG
Sse8387I 1865 CCTGCAGG CCTGCA^GG
XmaCI 36 CCCGGG C^CCGGG
XmaI 36 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AspI 164 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
901 GACAAGGTC
BbvCI 130 CCTCAGC CCTCAGC(-5/-2)
1823 CCTCAGC
BsrBI 1224 CCGCTC CCGCTC(-3/-3)
707 GAGCGG
BstAPI 1868 GCAGGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3666 GCAAGGACTGC
BstYI 1174 AGATCC [GA]^GATC[CT]
4419 AGATCT
DraIII 1287 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
4695 CACCCAGTG
HincII 1607 GTTGAC GT[CT]^[GA]AC
3757 GTCGAC
HindII 1607 GTTGAC GT[CT]^[GA]AC
3757 GTCGAC
MflI 1174 AGATCC [GA]^GATC[CT]
4419 AGATCT
PflFI 164 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
901 GACAAGGTC
PflMI 1154 CCAAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2437 CCAGCTCATGG
SphI 914 GCATGC GCATG^C
3870 GCATGC
Tth111I 164 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
901 GACAAGGTC
Van91I 1154 CCAAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2437 CCAGCTCATGG
XhoII 1174 AGATCC [GA]^GATC[CT]
4419 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI AseI AsnI
Asp700I AspHI AvaII BalI BanI
BanII BbsI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PshBI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XmnI