##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AccIII AclI AflIII AgeI AhdI
Alw44I ApaLI AscI AseI AsnI
AspEI AviII BamHI BcgI BglII
BsaBI BseAI BsiWI BsmBI BsmI
BspDI BspEI BspHI BspLU11I BssHII
BstBI BstEII BstPI ClaI CpoI
Eam1105I EcoO65I EcoRV EcoT22I FseI
FspI KspI MamI MfeI MluI
MroI MunI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI ScaI
SexAI SfiI SfuI SgrAI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AatII 341 GACGTC GACGT^C
AflII 5457 CTTAAG C^TTAAG
AocI 3233 CCTAAGG CC^T[ACGT]AGG
Aor51HI 2666 AGCGCT AGC^GCT
Asp700I 5082 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1207 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 4409 GTATCC GTATCC(6/5)
BfrI 5457 CTTAAG C^TTAAG
Bsp1407I 1237 TGTACA T^GTACA
BsrGI 1237 TGTACA T^GTACA
BssSI 1243 CTCGTG CACGAG(-5/-1)
Bst1107I 2852 GTATAC GTA^TAC
BstZ17I 2852 GTATAC GTA^TAC
Bsu36I 3233 CCTAAGG CC^T[ACGT]AGG
DraIII 1142 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 271 GACGGACCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2666 AGCGCT AGC^GCT
Eco81I 3233 CCTAAGG CC^T[ACGT]AGG
EcoRI 5731 GAATTC G^AATTC
NaeI 2328 GCCGGC GCC^GGC
NgoMIV 2328 GCCGGC G^CCGGC
NspI 2608 GCATGC [GA]CATG^[CT]
PflFI 1207 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SbfI 1082 CCTGCAGG CCTGCA^GG
SpeI 4538 ACTAGT A^CTAGT
SphI 2608 GCATGC GCATG^C
Sse8387I 1082 CCTGCAGG CCTGCA^GG
SspBI 1237 TGTACA T^GTACA
StuI 5264 AGGCCT AGG^CCT
TaqI 3484 TCGA T^CGA
Tth111I 1207 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 3484 TCGA T^CGA
XmnI 5082 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Acc65I 805 GGTACC G^GTACC
1341 GGTACC
Asp718I 805 GGTACC G^GTACC
1341 GGTACC
AvrII 3653 CCTAGG C^CTAGG
4488 CCTAGG
BbrPI 1142 CACGTG CAC^GTG
1166 CACGTG
BclI 5465 TGATCA T^GATCA
5624 TGATCA
BlnI 3653 CCTAGG C^CTAGG
4488 CCTAGG
BsrFI 1585 ACCGGC [GA]^CCGG[CT]
2328 GCCGGC
BstAPI 3179 GCACCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4828 GCAATCACTGC
Cfr10I 1585 ACCGGC [GA]^CCGG[CT]
2328 GCCGGC
DraI 4510 TTTAAA TTT^AAA
4746 TTTAAA
EcoNI 781 CCTGGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3520 CCTCCATGAGG
FbaI 5465 TGATCA T^GATCA
5624 TGATCA
HincII 4542 GTTAAC GT[CT]^[GA]AC
4959 GTTAAC
HindII 4542 GTTAAC GT[CT]^[GA]AC
4959 GTTAAC
HpaI 4542 GTTAAC GTT^AAC
4959 GTTAAC
KpnI 805 GGTACC GGTAC^C
1341 GGTACC
NdeI 4122 CATATG CA^TATG
4175 CATATG
PmaCI 1142 CACGTG CAC^GTG
1166 CACGTG
PmlI 1142 CACGTG CAC^GTG
1166 CACGTG
PshAI 1366 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2114 GACCTGCGTC
SacI 3421 GAGCTC GAGCT^C
4063 GAGCTC
SnaBI 5176 TACGTA TAC^GTA
5752 TACGTA
XcmI 875 CCAGCTGGGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4484 CCAGCCTAGGGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApaI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbeI BbsI BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HindIII HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI SmlI SspI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XhoII XmaCI XmaI