##################################
# NO CUT ENZYMES (   60 enzymes) #
##################################

AccI        AflII       AgeI        AhdI        AscI        
AseI        AsnI        AspEI       AvrII       BamHI       
BclI        BfrI        BlnI        BsiWI       BsrFI       
BssHII      Bst1107I    BstBI       BstZ17I     Cfr10I      
CpoI        EagI        Eam1105I    EclXI       Eco52I      
EcoRV       FbaI        FseI        MfeI        MluI        
MunI        NaeI        NdeI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PinAI       PmeI        PshBI       PvuI        RsrII       
SalI        SapI        SbfI        SfiI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        Sse8387I    
SwaI        XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AatII        3169  GACGTC               GACGT^C
Acc65I       1467  GGTACC               G^GTACC
AccIII       2277  TCCGGA               T^CCGGA
AclI           23  AACGTT               AA^CGTT
Alw44I        368  GTGCAC               G^TGCAC
ApaLI         368  GTGCAC               G^TGCAC
Asp718I      1467  GGTACC               G^GTACC
AviII        2388  TGCGCA               TGC^GCA
BciVI        1285  GGATAC               GTATCC(6/5)
BglI         2360  GCCCAGATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI        2365  GATGGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI        2277  TCCGGA               T^CCGGA
BsiEI        3832  CGGTCG               CG[GA][CT]^CG
Bsp1407I     1443  TGTACA               T^GTACA
BspDI         611  ATCGAT               AT^CGAT
BspEI        2277  TCCGGA               T^CCGGA
BspHI        4451  TCATGA               T^CATGA
BsrGI        1443  TGTACA               T^GTACA
BstEII       1792  GGTCACC              G^GT[ACGT]ACC
BstPI        1792  GGTCACC              G^GT[ACGT]ACC
ClaI          611  ATCGAT               AT^CGAT
DraI         4374  TTTAAA               TTT^AAA
DraIII       3239  CACCAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I      1792  GGTCACC              G^GT[ACGT]ACC
EcoRI        5058  GAATTC               G^AATTC
EcoT22I      2740  ATGCAT               ATGCA^T
FspI         2388  TGCGCA               TGC^GCA
HincII       3865  GTTAAC               GT[CT]^[GA]AC
HindII       3865  GTTAAC               GT[CT]^[GA]AC
HpaI         3865  GTTAAC               GTT^AAC
KpnI         1467  GGTACC               GGTAC^C
KspI         2973  CCGCGG               CCGC^GG
MamI         2365  GATGGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         2277  TCCGGA               T^CCGGA
NcoI         1989  CCATGG               C^CATGG
NsiI         2740  ATGCAT               ATGCA^T
PshAI        3487  GACTGGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I       23  AACGTT               AA^CGTT
RcaI         4451  TCATGA               T^CATGA
SacII        2973  CCGCGG               CCGC^GG
SphI         2742  GCATGC               GCATG^C
SspBI        1443  TGTACA               T^GTACA
StuI         3422  AGGCCT               AGG^CCT


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccII        2889  CGCG                 CG^CG
             2974  CGCG                
AflIII       2562  ACATGT               A^C[GA][CT]GT
             4099  ACATGT              
Aor51HI      2980  AGCGCT               AGC^GCT
             3814  AGCGCT              
ApaI         2178  GGGCCC               GGGCC^C
             2690  GGGCCC              
Asp700I      2560  GAACATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             5058  GAATTCCTTC          
BbeI         1725  GGCGCC               GGCGC^C
             3791  GGCGCC              
BcgI         1920  GCAAATCCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             4575  GCAGGAGAATCG        
BglII         788  AGATCT               A^GATCT
             2448  AGATCT              
BsmBI         847  CGTCTC               CGTCTC(1/5)
             4665  CGTCTC              
BspLU11I     2562  ACATGT               A^CATGT
             4099  ACATGT              
BstUI        2889  CGCG                 CG^CG
             2974  CGCG                
DrdI          539  GACGCTCATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3262  GACATGCGGGTC        
Eco47III     2980  AGCGCT               AGC^GCT
             3814  AGCGCT              
KasI         1725  GGCGCC               G^GCGCC
             3791  GGCGCC              
MvnI         2889  CGCG                 CG^CG
             2974  CGCG                
NarI         1725  GGCGCC               GG^CGCC
             3791  GGCGCC              
PflMI         189  CCAACGTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              803  CCACTCTCTGG         
SexAI        3240  ACCAGGT              A^CC[AT]GGT
             5134  ACCAGGT             
SfoI         1725  GGCGCC               GGC^GCC
             3791  GGCGCC              
SspI         3665  AATATT               AAT^ATT
             5180  AATATT              
TaqI          612  TCGA                 T^CGA
             1617  TCGA                
TthHB8I       612  TCGA                 T^CGA
             1617  TCGA                
Van91I        189  CCAACGTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              803  CCACTCTCTGG         
XmnI         2560  GAACATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             5058  GAATTCCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  131 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AluI        
AlwI        AlwNI       AocI        ApoI        AspHI       
AspI        AvaI        AvaII       BalI        BanI        
BanII       BbrPI       BbsI        BbvCI       BbvI        
BcnI        BfaI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaHI       BsaI        
BsaJI       BsaWI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsmI        
BsoBI       Bsp1286I    BspMI       BsrBI       BsrDI       
BsrI        BssKI       BssSI       BstAPI      BstF5I      
BstNI       BstXI       BstYI       Bsu36I      Cac8I       
CelII       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        Eco81I      
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HindIII     
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PflFI       PleI        PmaCI       PmlI        PpuMI       
PstI        PvuII       RsaI        SacI        Sau3AI      
Sau96I      ScaI        ScrFI       SfaNI       SfcI        
SmlI        StyI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       Tth111I     XcmI        
XhoII