##################################
# NO CUT ENZYMES (   62 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AgeI        
AhdI        AscI        AseI        AsnI        Asp718I     
AspEI       BciVI       BclI        BfrI        BglI        
BsaBI       BsiWI       BspDI       BsrBI       BssHII      
Bst1107I    BstZ17I     ClaI        CpoI        EagI        
Eam1105I    EclXI       Eco52I      FbaI        FseI        
HgaI        HpaI        KpnI        KspI        MamI        
MluI        NaeI        NgoMIV      NheI        NotI        
NruI        PacI        PflMI       PinAI       PshAI       
PshBI       Psp1406I    PvuII       RsrII       SacI        
SacII       SalI        SbfI        SfiI        SmaI        
SnaBI       SpeI        Sse8387I    SwaI        Van91I      
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccIII         64  TCCGGA               T^CCGGA
Aor51HI      4119  AGCGCT               AGC^GCT
ApaI         3373  GGGCCC               GGGCC^C
AviII         125  TGCGCA               TGC^GCA
AvrII        2688  CCTAGG               C^CTAGG
BamHI         345  GGATCC               G^GATCC
BcgI          806  GCAAAGCCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII         649  AGATCT               A^GATCT
BlnI         2688  CCTAGG               C^CTAGG
BlpI         4623  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     4623  GCTGAGC              GC^T[ACGT]AGC
BseAI          64  TCCGGA               T^CCGGA
BsiEI        2136  CGATCG               CG[GA][CT]^CG
Bsp1407I     5267  TGTACA               T^GTACA
BspEI          64  TCCGGA               T^CCGGA
BspHI        1092  TCATGA               T^CATGA
BspLU11I     1210  ACATGT               A^CATGT
BsrFI           8  GCCGGT               [GA]^CCGG[CT]
BsrGI        5267  TGTACA               T^GTACA
BssSI        3160  CTCGTG               CACGAG(-5/-1)
CelII        4623  GCTGAGC              GC^T[ACGT]AGC
Cfr10I          8  GCCGGT               [GA]^CCGG[CT]
DraIII        491  CACTCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI           80  GACACGGAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     4119  AGCGCT               AGC^GCT
EcoRV         902  GATATC               GAT^ATC
FspI          125  TGCGCA               TGC^GCA
HindIII      1565  AAGCTT               A^AGCTT
MfeI           40  CAATTG               C^AATTG
MroI           64  TCCGGA               T^CCGGA
MspA1I       3985  CAGCGG               C[AC]G^C[GT]G
MunI           40  CAATTG               C^AATTG
NdeI         3991  CATATG               CA^TATG
PaeR7I        166  CTCGAG               C^TCGAG
PmeI         3700  GTTTAAAC             GTTT^AAAC
PvuI         2136  CGATCG               CGAT^CG
RcaI         1092  TCATGA               T^CATGA
ScaI         1769  AGTACT               AGT^ACT
SgrAI           7  CGCCGGTG             C[GA]^CCGG[CT]G
SphI         2217  GCATGC               GCATG^C
SspBI        5267  TGTACA               T^GTACA
XbaI         5303  TCTAGA               T^CTAGA
XcmI         4535  CCAGAGGCCGACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          166  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccI         1634  GTAGAC               GT^[AC][GT]AC
             1697  GTAGAC              
AcyI          920  GGCGCC               G[GA]^CG[CT]C
             3070  GGCGCC              
Alw44I       1258  GTGCAC               G^TGCAC
             1678  GTGCAC              
ApaLI        1258  GTGCAC               G^TGCAC
             1678  GTGCAC              
BanII        3373  GGGCCC               G[GA]GC[CT]^C
             4837  GGGCTC              
BbeI          920  GGCGCC               GGCGC^C
             3070  GGCGCC              
BbrPI        1177  CACGTG               CAC^GTG
             1675  CACGTG              
BsaHI         920  GGCGCC               G[GA]^CG[CT]C
             3070  GGCGCC              
BsaWI          64  TCCGGA               [AT]^CCGG[AT]
             1192  ACCGGA              
BspMI        2873  ACCTGC               ACCTGC(4/8)
             3737  GCAGGT              
BstEII       3825  GGTTACC              G^GT[ACGT]ACC
             4372  GGTCACC             
BstPI        3825  GGTTACC              G^GT[ACGT]ACC
             4372  GGTCACC             
EcoO65I      3825  GGTTACC              G^GT[ACGT]ACC
             4372  GGTCACC             
EcoT22I       619  ATGCAT               ATGCA^T
             4038  ATGCAT              
Hin1I         920  GGCGCC               G[GA]^CG[CT]C
             3070  GGCGCC              
KasI          920  GGCGCC               G^GCGCC
             3070  GGCGCC              
NarI          920  GGCGCC               GG^CGCC
             3070  GGCGCC              
NsiI          619  ATGCAT               ATGCA^T
             4038  ATGCAT              
PmaCI        1177  CACGTG               CAC^GTG
             1675  CACGTG              
PmlI         1177  CACGTG               CAC^GTG
             1675  CACGTG              
SapI          913  GCTCTTC              GCTCTTC(1/4)
             4091  GCTCTTC             
SexAI        4368  ACCAGGT              A^CC[AT]GGT
             4615  ACCTGGT             
SfoI          920  GGCGCC               GGC^GCC
             3070  GGCGCC              
SspI         1867  AATATT               AAT^ATT
             4998  AATATT              


#########################################
# 3 OR MORE CUT ENZYMES (  128 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AflIII      
AluI        AlwI        AlwNI       AocI        ApoI        
Asp700I     AspHI       AspI        AvaI        AvaII       
BalI        BanI        BbsI        BbvCI       BbvI        
BcnI        BfaI        BmyI        BpmI        BpuAI       
BsaAI       BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1286I    BsrDI       
BsrI        BssKI       BstAPI      BstBI       BstF5I      
BstNI       BstUI       BstXI       BstYI       Bsu36I      
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraI        DraII       DsaI        EaeI        EarI        
Eco81I      EcoNI       EcoO109I    EcoRI       EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        HinP1I      HincII      
HindII      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspI        MvaI        MvnI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       NspI        NspV        PflFI       PleI        
PpuMI       PstI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SfuI        SmlI        
StuI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       Tth111I     
TthHB8I     XhoII       XmnI