##################################
# NO CUT ENZYMES (   44 enzymes) #
##################################

AatII       AflII       AgeI        AscI        AseI        
AsnI        AviII       BamHI       BbeI        BclI        
BfrI        BsiWI       BspDI       BssSI       Bst1107I    
BstBI       BstZ17I     ClaI        DrdI        EcoRV       
FbaI        FseI        FspI        KasI        MfeI        
MluI        MunI        NarI        NdeI        NotI        
NruI        NspV        PacI        PinAI       PmeI        
PshBI       PvuI        SfiI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        SwaI        


###################################
# ONE CUT ENZYMES (   23 enzymes) #
###################################

AccI         3166  GTCGAC               GT^[AC][GT]AC
AclI         2755  AACGTT               AA^CGTT
Aor51HI      1197  AGCGCT               AGC^GCT
AvrII        3046  CCTAGG               C^CTAGG
BlnI         3046  CCTAGG               C^CTAGG
BsaI         1734  GAGACC               GGTCTC(1/5)
BspLU11I     6397  ACATGT               A^CATGT
BssHII         13  GCGCGC               G^CGCGC
CpoI         2330  CGGTCCG              CG^G[AT]CCG
DraIII       6451  CACCTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III     1197  AGCGCT               AGC^GCT
EcoNI        3149  CCTGGGCTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HindIII      3533  AAGCTT               A^AGCTT
KspI         6362  CCGCGG               CCGC^GG
NaeI          642  GCCGGC               GCC^GGC
NgoMIV        642  GCCGGC               G^CCGGC
Psp1406I     2755  AACGTT               AA^CGTT
RsrII        2330  CGGTCCG              CG^G[AT]CCG
SacII        6362  CCGCGG               CCGC^GG
SalI         3166  GTCGAC               G^TCGAC
SbfI         1970  CCTGCAGG             CCTGCA^GG
SexAI        5333  ACCAGGT              A^CC[AT]GGT
Sse8387I     1970  CCTGCAGG             CCTGCA^GG


###################################
# TWO CUT ENZYMES (   42 enzymes) #
###################################

Acc65I        424  GGTACC               G^GTACC
              549  GGTACC              
AccIII        371  TCCGGA               T^CCGGA
             5130  TCCGGA              
AcyI         1757  GACGCC               G[GA]^CG[CT]C
             3006  GACGCC              
AhdI         3006  GACGCCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             6039  GACTGATGGTC         
AlwI          404  GGATC                GGATC(4/5)
             1509  GGATC               
AocI          148  CCTTAGG              CC^T[ACGT]AGG
              419  CCTGAGG             
ApaI         1041  GGGCCC               GGGCC^C
             1964  GGGCCC              
Asp700I      1271  GAATCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4900  GAAACTTTTC          
Asp718I       424  GGTACC               G^GTACC
              549  GGTACC              
AspEI        3006  GACGCCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             6039  GACTGATGGTC         
BbvCI        3660  CCTCAGC              CCTCAGC(-5/-2)
             1200  GCTGAGG             
BciVI        5473  GGATAC               GTATCC(6/5)
              595  GTATCC              
BsaBI        3251  GATGTAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             4224  GATGTAAATC          
BsaHI        1757  GACGCC               G[GA]^CG[CT]C
             3006  GACGCC              
BseAI         371  TCCGGA               T^CCGGA
             5130  TCCGGA              
BsiEI         640  CGGCCG               CG[GA][CT]^CG
             1744  CGGCCG              
BspEI         371  TCCGGA               T^CCGGA
             5130  TCCGGA              
BspHI        4212  TCATGA               T^CATGA
             6032  TCATGA              
BstAPI       2239  GCATTGTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3665  GCAGAGTTTGC         
BstEII        995  GGTCACC              G^GT[ACGT]ACC
             2628  GGTCACC             
BstPI         995  GGTCACC              G^GT[ACGT]ACC
             2628  GGTCACC             
BstXI        1156  CCAGCCCCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4647  CCATTACAGTGG        
Bsu36I        148  CCTTAGG              CC^T[ACGT]AGG
              419  CCTGAGG             
EagI          640  CGGCCG               C^GGCCG
             1744  CGGCCG              
Eam1105I     3006  GACGCCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             6039  GACTGATGGTC         
EclXI         640  CGGCCG               C^GGCCG
             1744  CGGCCG              
Eco52I        640  CGGCCG               C^GGCCG
             1744  CGGCCG              
Eco81I        148  CCTTAGG              CC^T[ACGT]AGG
              419  CCTGAGG             
EcoO65I       995  GGTCACC              G^GT[ACGT]ACC
             2628  GGTCACC             
EcoRI        1415  GAATTC               G^AATTC
             5787  GAATTC              
Hin1I        1757  GACGCC               G[GA]^CG[CT]C
             3006  GACGCC              
HpaI         4868  GTTAAC               GTT^AAC
             6314  GTTAAC              
KpnI          424  GGTACC               GGTAC^C
              549  GGTACC              
MamI         3251  GATGTAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             4224  GATGTAAATC          
MroI          371  TCCGGA               T^CCGGA
             5130  TCCGGA              
NheI         3730  GCTAGC               G^CTAGC
             5622  GCTAGC              
PshAI        1664  GACCAAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             6088  GACTGCTGTC          
RcaI         4212  TCATGA               T^CATGA
             6032  TCATGA              
SmaI         1117  CCCGGG               CCC^GGG
             1177  CCCGGG              
XmaCI        1117  CCCGGG               C^CCGGG
             1177  CCCGGG              
XmaI         1117  CCCGGG               C^CCGGG
             1177  CCCGGG              
XmnI         1271  GAATCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4900  GAAACTTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  149 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AflIII      
AluI        Alw44I      AlwNI       ApaLI       ApoI        
AspHI       AspI        AvaI        AvaII       BalI        
BanI        BanII       BbrPI       BbsI        BbvI        
BcgI        BcnI        BfaI        BglI        BglII       
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaJI       BsaWI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1286I    Bsp1407I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstYI       Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraI        DraII       
DsaI        EaeI        EarI        EcoO109I    EcoRII      
EcoT14I     EcoT22I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
HinP1I      HincII      HindII      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       NsiI        
NspI        PaeR7I      PflFI       PflMI       PleI        
PmaCI       PmlI        PpuMI       PstI        PvuII       
RsaI        SacI        SapI        Sau3AI      Sau96I      
ScaI        ScrFI       SfaNI       SfcI        SmlI        
SphI        SspBI       SspI        StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XhoII