##################################
# NO CUT ENZYMES (   66 enzymes) #
##################################

AatII       AccIII      AclI        AgeI        Alw44I      
Aor51HI     ApaLI       AscI        AspI        AviII       
BbeI        BglI        BsaBI       BseAI       BsiEI       
BsiWI       BsmBI       BspDI       BspEI       BspHI       
BsrFI       BssHII      Bst1107I    BstBI       BstEII      
BstPI       BstZ17I     Cfr10I      ClaI        CpoI        
DrdI        EagI        EclXI       Eco47III    Eco52I      
EcoO65I     FseI        FspI        KasI        KspI        
MamI        MluI        MroI        NaeI        NarI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PflFI       PinAI       PmeI        Psp1406I    
PvuI        RcaI        RsrII       SacII       SfiI        
SfoI        SfuI        SgrAI       SnaBI       SphI        
Tth111I     


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

Acc65I       4239  GGTACC               G^GTACC
AhdI         3638  GACATTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         3980  CCTAAGG              CC^T[ACGT]AGG
Asp700I      5523  GAATACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      4239  GGTACC               G^GTACC
AspEI        3638  GACATTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII        4894  CCTAGG               C^CTAGG
BclI         4435  TGATCA               T^GATCA
BglII        3087  AGATCT               A^GATCT
BlnI         4894  CCTAGG               C^CTAGG
BsaWI        2556  ACCGGA               [AT]^CCGG[AT]
BsrBI        1151  GAGCGG               CCGCTC(-3/-3)
BstAPI       7050  GCAGCTGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I       3980  CCTAAGG              CC^T[ACGT]AGG
DsaI         4696  CCATGG               C^C[GA][CT]GG
Eam1105I     3638  GACATTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       3980  CCTAAGG              CC^T[ACGT]AGG
FbaI         4435  TGATCA               T^GATCA
HaeII         886  AGCGCC               [GA]GCGC^[CT]
HpaI          943  GTTAAC               GTT^AAC
KpnI         4239  GGTACC               GGTAC^C
MfeI         2952  CAATTG               C^AATTG
MunI         2952  CAATTG               C^AATTG
NcoI         4696  CCATGG               C^CATGG
NdeI         1920  CATATG               CA^TATG
PaeR7I       1719  CTCGAG               C^TCGAG
SacI         3597  GAGCTC               GAGCT^C
SalI         3030  GTCGAC               G^TCGAC
SbfI         1221  CCTGCAGG             CCTGCA^GG
ScaI         1407  AGTACT               AGT^ACT
SexAI         204  ACCAGGT              A^CC[AT]GGT
SmaI         4023  CCCGGG               CCC^GGG
SpeI         5916  ACTAGT               A^CTAGT
Sse8387I     1221  CCTGCAGG             CCTGCA^GG
XhoI         1719  CTCGAG               C^TCGAG
XmaCI        4023  CCCGGG               C^CCGGG
XmaI         4023  CCCGGG               C^CCGGG
XmnI         5523  GAATACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AvaI         1719  CTCGAG               C^[CT]CG[GA]G
             4023  CCCGGG              
BalI         2536  TGGCCA               TGG^CCA
             7699  TGGCCA              
BbrPI         779  CACGTG               CAC^GTG
             4781  CACGTG              
BcgI         4508  CGACACCATTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             4901  GCAACACTTTCG        
BciVI        5923  GGATAC               GTATCC(6/5)
             2266  GTATCC              
BsaI         4692  GAGACC               GGTCTC(1/5)
             6421  GGTCTC              
BsoBI        1719  CTCGAG               C^[CT]CG[GA]G
             4023  CCCGGG              
BspMI        1366  ACCTGC               ACCTGC(4/8)
             3482  ACCTGC              
BssSI        2116  CACGAG               CACGAG(-5/-1)
             2751  CACGAG              
BstXI        3218  CCACCAACCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             7418  CCATCCGTCTGG        
EaeI         2536  TGGCCA               [CT]^GGCC[GA]
             7699  TGGCCA              
MluNI        2536  TGGCCA               TGG^CCA
             7699  TGGCCA              
MscI         2536  TGGCCA               TGG^CCA
             7699  TGGCCA              
PmaCI         779  CACGTG               CAC^GTG
             4781  CACGTG              
PmlI          779  CACGTG               CAC^GTG
             4781  CACGTG              
PshAI        4086  GACGCTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             4694  GACCATGGTC          
SapI         1380  GAAGAGC              GCTCTTC(1/4)
             4534  GCTCTTC             
SwaI         6471  ATTTAAAT             ATTT^AAAT
             7511  ATTTAAAT            
XcmI         3354  CCAACCCCAAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             7021  CCAAAACCCTCATGG     


#########################################
# 3 OR MORE CUT ENZYMES (  135 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AcyI        
AfaI        AflII       AflIII      AluI        AlwI        
AlwNI       ApaI        ApoI        AseI        AsnI        
AspHI       AvaII       BamHI       BanI        BanII       
BbsI        BbvCI       BbvI        BcnI        BfaI        
BfrI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaHI       BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        Bsp1286I    Bsp1407I    BspLU11I    
BsrDI       BsrGI       BsrI        BssKI       BstF5I      
BstNI       BstUI       BstYI       Cac8I       CelII       
CfoI        Cfr13I      DdeI        DpnII       DraI        
DraII       DraIII      EarI        EcoNI       EcoO109I    
EcoRI       EcoRII      EcoRV       EcoT14I     EcoT22I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NsiI        NspI        PflMI       PleI        PpuMI       
PshBI       PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
SspBI       SspI        StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XbaI        XhoII