##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII AflIII AgeI AhdI Aor51HI
AscI AseI AsnI AspEI AspI
BclI BsaBI BsiWI Bsp1407I BspDI
BspHI BspLU11I BsrGI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DrdI Eam1105I Eco47III EcoO65I EcoRV
FbaI HpaI MamI MfeI MluI
MunI NdeI NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI PvuI RcaI
RsrII SalI SfuI SnaBI SpeI
SspBI SspI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 5180 GGTACC G^GTACC
AccI 1875 GTAGAC GT^[AC][GT]AC
AccIII 3031 TCCGGA T^CCGGA
AclI 1479 AACGTT AA^CGTT
AflII 4897 CTTAAG C^TTAAG
Alw44I 4614 GTGCAC G^TGCAC
ApaLI 4614 GTGCAC G^TGCAC
Asp700I 4938 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 5180 GGTACC G^GTACC
AviII 1336 TGCGCA TGC^GCA
AvrII 4916 CCTAGG C^CTAGG
BbrPI 4734 CACGTG CAC^GTG
BbvCI 4811 GCTGAGG CCTCAGC(-5/-2)
BfrI 4897 CTTAAG C^TTAAG
BlnI 4916 CCTAGG C^CTAGG
BsaAI 4734 CACGTG [CT]AC^GT[GA]
BseAI 3031 TCCGGA T^CCGGA
BspEI 3031 TCCGGA T^CCGGA
BsrDI 5196 CATTGC GCAATG(2/0)
BssHII 62 GCGCGC G^CGCGC
BssSI 2188 CTCGTG CACGAG(-5/-1)
DraIII 3001 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 2340 GAATTC G^AATTC
EcoT22I 599 ATGCAT ATGCA^T
FspI 1336 TGCGCA TGC^GCA
HincII 2196 GTTGAC GT[CT]^[GA]AC
HindII 2196 GTTGAC GT[CT]^[GA]AC
HindIII 4900 AAGCTT A^AGCTT
KpnI 5180 GGTACC GGTAC^C
MroI 3031 TCCGGA T^CCGGA
NsiI 599 ATGCAT ATGCA^T
PmaCI 4734 CACGTG CAC^GTG
PmlI 4734 CACGTG CAC^GTG
Psp1406I 1479 AACGTT AA^CGTT
SapI 1895 GCTCTTC GCTCTTC(1/4)
SbfI 2213 CCTGCAGG CCTGCA^GG
ScaI 2500 AGTACT AGT^ACT
SexAI 3002 ACCAGGT A^CC[AT]GGT
SfiI 3326 GGCCGGTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 4818 CACCGGCG C[GA]^CCGG[CT]G
SphI 869 GCATGC GCATG^C
Sse8387I 2213 CCTGCAGG CCTGCA^GG
XbaI 4946 TCTAGA T^CTAGA
XmnI 4938 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AcsI 2340 GAATTC [GA]^AATT[CT]
3853 AAATTC
ApoI 2340 GAATTC [GA]^AATT[CT]
3853 AAATTC
BcgI 2459 CGAGGAGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4107 CGAAGGCTCTGC
BciVI 1560 GTATCC GTATCC(6/5)
1822 GTATCC
BglII 5116 AGATCT A^GATCT
5189 AGATCT
BsaWI 3031 TCCGGA [AT]^CCGG[AT]
5470 TCCGGT
BsmBI 1424 CGTCTC CGTCTC(1/5)
1721 CGTCTC
DraI 5226 TTTAAA TTT^AAA
5382 TTTAAA
EcoNI 1007 CCTGGCGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2574 CCTGTGCCAGG
FseI 351 GGCCGGCC GGCCGG^CC
355 GGCCGGCC
KspI 21 CCGCGG CCGC^GG
91 CCGCGG
SacII 21 CCGCGG CCGC^GG
91 CCGCGG
StuI 3349 AGGCCT AGG^CCT
4355 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BamHI BanI
BanII BbeI BbsI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI