KIAA1174

Product ID	ORK05843
Accession No	AB033000
Description	limb development membrane protein 1-like
Clone name	hh05825a
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (2800 bp) Predicted protein sequence (430 aa)
Source	Human adult brain
Rouge ID	mKIAA1174 by Kazusa Mouse cDNA Project

Length: 2800 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	1506 bp
Genome contig ID	gi89161190r_47677208
PolyA signal sequence (AATAAA,-19)	+--------+--------+----*----+---- GGCTTCAGCCATCAGCAATAAAGTTGATCCCAGGG
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* TTTGCTTTGTTTTTTTAAAGCACCTGTCTCTGTGTATATTTGAACAGCCT

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	12	r	47777208	47790717	15	100.0	Perfect prediction

Length: 430 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
EAW58044	2.8e-164	100.0	limb region 1 h...	Homo sapiens
EDL04155	3e-153	89.9	limb region 1 l...	Mus musculus
Q6UX01	5e-150	95.5	Protein LMBR1L;...	Homo sapiens
XP_509041	1.3e-149	95.0	lipocalin-inter...	Pan troglodytes
AAQ88935	1.3e-149	95.3	EAPD458 [Homo s...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR008075	107	129	PR01692	Lipocalin-1 receptor
	IPR008075	149	171	PR01692	Lipocalin-1 receptor
	IPR008075	229	250	PR01692	Lipocalin-1 receptor
	IPR008075	336	357	PR01692	Lipocalin-1 receptor
	IPR008075	394	414	PR01692	Lipocalin-1 receptor
HMMPfam	IPR006876	62	430	PF04791	LMBR1-like conserved region

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	1	CEERPLGWVWVLGGGGFLPARP	22	SECONDARY	22
	2	58	RECIISTLLFATLYILCHIFLTR	80	PRIMARY	23
	3	104	LCTFTLAIALGAVLLLPFSIISN	126	PRIMARY	23
	4	148	GLWNLVFLFSNLSLIFLMPFAYF	170	PRIMARY	23
	5	191	TVVMLMLLTLLVLGMVWVASAIV	213	PRIMARY	23
	6	237	CISFLGVLLLLVCTPLGLARMFS	259	PRIMARY	23
	7	340	MLCLLVLTGLSVLIVAIHILELL	362	PRIMARY	23
	8	383	SKLGSFGAVIQVVLILYPSGNPS	405	PRIMARY	23

Primer_f	CTGAGCAGAGTATGGAAGCAC
Primer_r	AAGATGTGGCAGAGGATGTAC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	AGGCTCGGGAGATAGATTGTC
Primer_r	CCTATCCCCTTGGTCTTTCAG
PCR product length	104 bp
PCR conditions	95 °C15 sec64 °C60 sec30 cycles