KIAA0241

Product ID	ORK05586
Accession No	D87682
Description	AVL9 homolog (S. cerevisiase)
Clone name	ha04847
Vector information	The cDNA fragment was inserted at the EcoRV-NotI site of the ... The cDNA fragment was inserted at the EcoRV-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the EcoRV (blunt-end ligation) site without using any linker or adapter. The 3' end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (6371 bp) Predicted protein sequence (522 aa)
Source	Myeloblast cell line (KG-1)
Rouge ID	mKIAA0241 by Kazusa Mouse cDNA Project

Length: 6371 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	4802 bp
Genome contig ID	gi89161213f_32450829
PolyA signal sequence (AATAAA,-26)	+--------+--------+----*----+---- ATAAATGAGAATAAAATGATCCTGTTCACCCTGCC
Flanking genome sequence (144021 - 144070)	----+--------+--------+--------+--------+----* TACAGTTGGACTTGACCTGTCCTAGAAGTACCCTGTGTGTTGTCCATGTG

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	7	f	32550824	32594848	12	99.1	Perfect prediction

Length: 522 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
XP_001082573	4e-181	87.9	similar to CG11...	Macaca mulatta
XP_539507	2.4e-170	81.9	similar to CG11...	Canis lupus fam...
XP_132538	3.9e-165	81.0	hypothetical pr...	Mus musculus
EDL88053	1.4e-164	80.9	rCG52383 [Rattu...	Rattus norvegicus
EDL04714	4.6e-157	90.3	mCG13107 [Mus m...	Mus musculus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
None	-	-	-	-	-

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	134	KVLFYISPVNKLVGALMTVLSLF	156	PRIMARY	23
SOSUI2	2	438	AQFAVYIHALLAATLQLVLFRIV	460	PRIMARY	23

Primer_f
Primer_r
PCR conditions	test

Panel name	Genebridge 4
Primer_f	GTGAAGGCATTTAGTGGTTAC
Primer_r	TACCAGAGCAAAGAGAACTAC
PCR product length	165 bp
PCR conditions	95 °C15 sec60 °C60 sec30 cycles