Gene/Protein Characteristic Table for KIAA1535
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Product ID ORK05401
Accession No AB040968
Description hyperpolarization activated cyclic nucleotide gated potassium channel 3
Clone name fk12792
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (3522 bp)
Predicted protein sequence (711 aa)
Source Human fetal brain
Note We replaced fh09002, former representative clones for KIAA1535 with fk12792. (2005/08/06)
Features of the cloned cDNA sequence
Description

Length: 3522 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq Warning No warning
Integrity of 3' end
Length of 3'UTR 1384 bp
Genome contig ID gi89161185f_153414193
PolyA signal sequence
(TATAAA,-18)
+----*----+----*----+----*----+----
AAACAGCTGGGTTTAAATATAAAATAGACACACTC
Flanking genome sequence
(112071 - 112120)
----+----*----+----*----+----*----+----*----+----*
ATTTTTGGCTCTTGGTTTGTGTGTGGGAACAACATGAGTGGGAAGGAATT

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 1 f 153514193 153526262 8 99.4 Perfect prediction
Features of the protein sequence
Description

Length: 711 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
Q9P1Z3 0 99.9 Potassium/sodiu...
Homo sapiens
AAO49470 0 99.6 hyperpolarizati...
Homo sapiens
XP_001115891 0 98.5 hyperpolarizati...
Macaca mulatta
XP_001498862 0 97.5 similar to hype...
Equus caballus
XP_588153 0 96.8 similar to Pota...
Bos taurus
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB033108 5.8e-05 25.1 KIAA1282
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
FPrintScan IPR003938 25 34 PR01463 EAG/ELK/ERG potassium channel
IPR003938 67 77 PR01463 EAG/ELK/ERG potassium channel
IPR003938 78 87 PR01463 EAG/ELK/ERG potassium channel
IPR003938 197 207 PR01463 EAG/ELK/ERG potassium channel
IPR003938 279 288 PR01463 EAG/ELK/ERG potassium channel
IPR003938 443 451 PR01463 EAG/ELK/ERG potassium channel
HMMPfam IPR013621 1 61 PF08412 Ion transport N-terminal
IPR005821 65 283 PF00520 Ion transport
IPR000595 383 468 PF00027 Cyclic nucleotide-binding
HMMSmart IPR000595 365 478 SM00100 Cyclic nucleotide-binding
ProfileScan IPR000595 365 471 PS50042 Cyclic nucleotide-binding

Prediction of transmembrane (TM) segments
Method No. N terminal transmembrane region C terminal type length
SOSUI2 1 25 PYSDFRFYWDLIMLLLMVGNLIV 47 SECONDARY 23
2 180 DLASAVVRIFNLIGMMLLLCHWD 202 PRIMARY 23
3 263 WLTMLSMIVGATCYAMFIGHATA 285 SECONDARY 23
Expression profile
Description

RT-PCR-ELISA
Experimental conditions
Primer_f CTTCTGAGTTTGCTGTTGGTG
Primer_r TGGTCCCACAGTCTTTAATGC
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 1
Experimental conditions
Panel name GeneBridge 4
Primer_f CTTCTGAGTTTGCTGTTGGTG
Primer_r TGGTCCCACAGTCTTTAATGC
PCR product length 171 bp
PCR conditions 95 °C15 sec62 °C60 sec30 cycles
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