FLJ00074

Product ID	ORK07544
Accession No	AK024480
Clone name	as00074
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4856 bp) Predicted protein sequence (191 aa)
Source	Human spleen

Length: 4856 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Length: 191 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAB15770	8.4e-76	100.0	FLJ00074 protei...	Homo sapiens
CAI23420	1.1e-75	100.0	novel protein [...	Homo sapiens
Q5TF58	1.2e-75	100.0	Intermediate fi...	Homo sapiens
CAI23419	1.4e-75	100.0	novel protein [...	Homo sapiens
EAW94862	1.9e-75	100.0	hCG25110 [Homo ...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other entries from Kazusa human cDNA project (FASTA output, Multiple alignment)

No significant homologues

RT-PCR-ELISA

Experimental conditions

Primer_f	AGTCAGCATCTCTTGTTAGCC
Primer_r	ACTGGCTCTGATAACCTTTGG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles