KIAA1265

Product ID	ORK00208
Accession No	AB033091
Description	solute carrier family 39 (zinc transporter), member 10, transcript variant 2
Clone name	hj04493
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5231 bp) Predicted protein sequence (835 aa)
HaloTag ORF Clone	FHC00208
Flexi ORF Clone	FXC00208
Source	Human adult brain
Rouge ID	mKIAA1265 by Kazusa Mouse cDNA Project

Length: 5231 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	2657 bp
Genome contig ID	gi89161199f_196130184
PolyA signal sequence (AATAAA,-19)	+--------+--------+----*----+---- TATGGAACTCCTCCAAAATAAAGTTACTCAAAGAG
Flanking genome sequence (180481 - 180530)	----+--------+--------+--------+--------+----* AGCAAATATGCCAGTGTGTTTTCTTTAGTCTTTATTTCAGAACATTTCCT

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	2	f	196230184	196310663	10	99.5	Perfect prediction

Length: 835 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
XP_515998	99.8	solute carrier ...	Pan troglodytes
XP_001085289	99.5	similar to solu...	Macaca mulatta
Q9ULF5	100.0	Zinc transporte...	Homo sapiens
CAH18673	99.9	hypothetical pr...	Homo sapiens
XP_001502432	92.8	solute carrier ...	Equus caballus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
D31887	1.8e-23	31.2	KIAA0062

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR003689	408	823	PF02535	Zinc/iron permease
ScanRegExp	IPR007087	14	35	PS00028	Zinc finger

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	11	TKFCLICLLTFIFHHCNHCHEE	32	SECONDARY	22
	2	410	AWICGIISITVISLLSLLGVILV	432	PRIMARY	23
	3	443	LLTFLVALAVGTMSGDALLHLLP	465	SECONDARY	23
	4	497	DAVLKGLVALGGIYLLFIIEHCI	519	SECONDARY	23
	5	691	AIGAAFSAGLTGGISTSIAVFCH	713	SECONDARY	23
	6	738	YNLLSAMMAYIGMLIGTAVGQYA	760	PRIMARY	23
	7	764	TLWIFAVTAGMFLYVALVDMLPE	786	PRIMARY	23
	8	801	PVGQFILQNLGLLFGFAIMLVIA	823	PRIMARY	23

Primer_f	CATCTCATTGCTTGGCCCTTC
Primer_r	AATGCAGAATCCTACCGTGGG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	CATCTCATTGCTTGGCCCTTC
Primer_r	AATGCAGAATCCTACCGTGGG
PCR product length	165 bp
PCR conditions	95 °C15 sec64 °C60 sec30 cycles