Gene/Protein Characteristic Table for KIAA1089
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Product ID ORK00736
Accession No AB029012
Description SMG5 nonsense mediated mRNA decay factor
Clone name ha02541
Vector information
The cDNA fragment was inserted at the EcoRV-NotI site of the ...
cDNA sequence DNA sequence (4563 bp)
Predicted protein sequence (1033 aa)
Flexi ORF Clone FXC00736
Source Myeloblast cell line (KG-1)
Rouge ID mKIAA1089 by Kazusa Mouse cDNA Project
Note We replaced hk03401, former representative clones for KIAA1089 with ha02541. (2002/5/10)
Features of the cloned cDNA sequence
Description

Length: 4563 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Integrity of 3' end
Length of 3'UTR 1361 bp
Genome contig ID gi89161185r_154385641
PolyA signal sequence
(AATAAA,-18)
+----*----+----*----+----*----+----
AGACTAAAGCATCACTTAATAAACCCCCCAAGCCC
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
AATCCCTGTCTCCTGGTGCCTGTCTGTACAGAAGTTTCATTGGGGGGTGG

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 1 r 154485641 154519233 22 99.6 Terminal No-hit
Features of the protein sequence
Description

Length: 1033 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
XP_513880 0 99.6 Est1p-like prot...
Pan troglodytes
Q9UPR3 0 100.0 Protein SMG5; S...
Homo sapiens
AAH38296 0 99.9 Smg-5 homolog, ...
Homo sapiens
AAQ84301 0 99.7 LPTS interactin...
Homo sapiens
AAI34681 0 96.2 SMG5 protein [B...
Bos taurus
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
D87437 7.1e-07 25.0 KIAA0250
AB018275 1.7e-05 24.8 KIAA0732
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMSmart IPR006596 872 995 SM00670 Nucleotide binding protein

Prediction of transmembrane (TM) segments
Method No. N terminal transmembrane region C terminal type length
SOSUI2 1 365 AFLPDLLIFQMVIICLMCVHSLE 387 PRIMARY 23
Expression profile
Description

RT-PCR-ELISA
Experimental conditions
Primer_f GGAGCAGAGATCATGAATAGC
Primer_r GCCAACTATCCTCTAAGCCAC
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 1
Experimental conditions
Panel name GeneBridge 4
Primer_f GGAGCAGAGATCATGAATAGC
Primer_r GCCAACTATCCTCTAAGCCAC
PCR product length 211 bp
PCR conditions 95 °C15 sec66 °C60 sec30 cycles
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