Gene/Protein Characteristic Table for KIAA1083
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Product ID ORK00733
Accession No AB029006
Description spastin, transcript variant 2
Clone name hj07423
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (5120 bp)
Predicted protein sequence (584 aa)
Flexi ORF Clone FXC00733
Source Human adult brain
Rouge ID mKIAA1083 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 5120 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Integrity of 3' end
Length of 3'UTR 3144 bp
Genome contig ID gi89161199f_32042184
PolyA signal sequence
(AATAAA,-17)
+----*----+----*----+----*----+----
TTATCCTAATGTATTGGAAATAAATGATAAACTTT
Flanking genome sequence
(194027 - 194076)
----+----*----+----*----+----*----+----*----+----*
ATTTTGTCTTTACTTTTTTACTTTAAAACTTTTTGATATTTTAGGGGTTG

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 2 f 32142184 32236209 16 99.4 Perfect prediction
Features of the protein sequence
Description

Length: 584 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
XP_862831 1.6e-175 94.9 similar to spas...
Canis lupus fam...
AAI66846 2.7e-169 93.8 Spast protein [...
Rattus norvegicus
XP_001371530 4.4e-150 90.3 similar to spas...
Monodelphis dom...
ACF60961 1.7e-131 87.9 neuronal spasti...
Gallus gallus
Q9UBP0 8.8e-118 94.8 Spastin.
Homo sapiens
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMPfam IPR003959 345 530 PF00004 AAA ATPase
HMMSmart IPR007330 116 194 SM00745 MIT
IPR003593 342 478 SM00382 AAA+ ATPase
ScanRegExp IPR003960 448 467 PS00674 AAA ATPase

Prediction of transmembrane (TM) segments
Method No. N terminal transmembrane region C terminal type length
SOSUI2 1 55 YPLFVGFALLRLVAFHLGLLFVW 77 PRIMARY 23
Expression profile
Description

RT-PCR-ELISA
Experimental conditions
Primer_f TCGGTGTTGAGCTCTGTGTTG
Primer_r ACTCATTCTTTCCCTTGGCTC
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 2
Experimental conditions
Panel name GeneBridge 4
Primer_f TCGGTGTTGAGCTCTGTGTTG
Primer_r ACTCATTCTTTCCCTTGGCTC
PCR product length 151 bp
PCR conditions 95 °C15 sec64 °C60 sec35 cycles
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