Gene/Protein Characteristic Table for KIAA0439
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Product ID ORK00077
Accession No AB007899
Description neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase, transcript variant c
Clone name hj00462
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (4879 bp)
Predicted protein sequence (995 aa)
Flexi ORF Clone FXC00077
Source Human adult brain
Rouge ID mKIAA0439 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 4879 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq Warning No warning
Features of the protein sequence
Description

Length: 995 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
NP_001138441 0 99.8 neural precurso...
Homo sapiens
BAB69424 0 99.8 NEDD4-like ubiq...
Homo sapiens
AAM76730 0 99.7 ubiquitin ligas...
Homo sapiens
Q5RBF2 0 99.5 E3 ubiquitin-pr...
Pongo abelii
XP_001064485 0 98.1 similar to E3 u...
Rattus norvegicus
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
D42055 1.5e-97 65.6 KIAA0093
AB037722 1.8e-50 36.4 KIAA1301
AB002320 1.4e-44 34.9 KIAA0322
AB046845 3.1e-44 44.2 KIAA1625
AB002310 7.9e-38 37.3 KIAA0312
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
FPrintScan IPR000008 77 89 PR00360 C2 calcium-dependent membrane targeting
IPR000008 108 121 PR00360 C2 calcium-dependent membrane targeting
HMMPfam IPR000008 79 149 PF00168 C2 calcium-dependent membrane targeting
IPR001202 235 264 PF00397 WW/Rsp5/WWP
IPR001202 407 436 PF00397 WW/Rsp5/WWP
IPR001202 519 548 PF00397 WW/Rsp5/WWP
IPR001202 570 599 PF00397 WW/Rsp5/WWP
IPR000569 689 994 PF00632 HECT
HMMSmart IPR000008 53 164 SM00239 C2 calcium-dependent membrane targeting
IPR001202 234 266 SM00456 WW/Rsp5/WWP
IPR001202 406 438 SM00456 WW/Rsp5/WWP
IPR001202 518 550 SM00456 WW/Rsp5/WWP
IPR001202 569 601 SM00456 WW/Rsp5/WWP
IPR000569 658 994 SM00119 HECT
ProfileScan IPR000008 69 149 PS50004 C2 calcium-dependent membrane targeting
IPR001202 233 266 PS50020 WW/Rsp5/WWP
IPR001202 405 438 PS50020 WW/Rsp5/WWP
IPR001202 517 550 PS50020 WW/Rsp5/WWP
IPR001202 568 601 PS50020 WW/Rsp5/WWP
IPR000569 660 994 PS50237 HECT
ScanRegExp IPR001202 239 264 PS01159 WW/Rsp5/WWP
IPR001202 411 436 PS01159 WW/Rsp5/WWP
IPR001202 523 548 PS01159 WW/Rsp5/WWP
IPR001202 574 599 PS01159 WW/Rsp5/WWP
Expression profile
Description

RT-PCR
Experimental conditions
Primer_f GGTCGTTCAACTCCCACACTG
Primer_r ACTTGACTTGATCCTCTGAGC
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 18
Experimental conditions
Panel name GeneBridge 4
Primer_f GGTCGTTCAACTCCCACACTG
Primer_r ACTTGACTTGATCCTCTGAGC
PCR product length 159 bp
PCR conditions 95 °C15 sec64 °C60 sec30 cycles
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