Gene/Protein Characteristic Table for KIAA0028
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Product ID ORK00374
Accession No D21851
Description leucyl-tRNA synthetase 2, mitochondrial
Clone name ha00560
Vector information
The cDNA fragment was inserted at the EcoRV-NotI site of the ...
cDNA sequence DNA sequence (4203 bp)
Predicted protein sequence (904 aa)
Flexi ORF Clone FXC00374
Source Myeloblast cell line (KG-1)
Features of the cloned cDNA sequence
Description

Length: 4203 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Integrity of 3' end
Length of 3'UTR 1306 bp
Genome contig ID gi89161205f_45305079
PolyA signal sequence
(AATAAA,-22)
+----*----+----*----+----*----+----
ACTTTTTTAGTGTAATAAAATGTTTATCATCTATG
Flanking genome sequence
(260255 - 260304)
----+----*----+----*----+----*----+----*----+----*
ACTTCAGGACATATGTGTGATCCTGGAATACACCATATTCCAATTTCAGA

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 3 f 45405079 45565332 22 99.9 Perfect prediction
Features of the protein sequence
Description

Length: 904 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
Q15031 0 100.0 Probable leucyl...
Homo sapiens
XP_001147524 0 99.7 leucyl-tRNA syn...
Pan troglodytes
Q5RDP4 0 99.2 Probable leucyl...
Pongo abelii
XP_001114552 0 96.0 leucyl-tRNA syn...
Macaca mulatta
XP_001500886 0 92.5 similar to Prob...
Equus caballus
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB037773 0.00028 26.4 KIAA1352
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
FPrintScan IPR002302 177 194 PR00985 Leucyl-tRNA synthetase
IPR002302 203 219 PR00985 Leucyl-tRNA synthetase
IPR002302 236 249 PR00985 Leucyl-tRNA synthetase
IPR002302 265 284 PR00985 Leucyl-tRNA synthetase
IPR002302 516 534 PR00985 Leucyl-tRNA synthetase
IPR002302 555 577 PR00985 Leucyl-tRNA synthetase
IPR002302 588 598 PR00985 Leucyl-tRNA synthetase
HMMPfam IPR015413 85 167 PF09334 Aminoacyl-tRNA synthetase
IPR002300 640 680 PF00133 Aminoacyl-tRNA synthetase
IPR013155 726 872 PF08264 Valyl/Leucyl/Isoleucyl-tRNA synthetase
HMMTigr IPR002302 55 903 TIGR00396 Leucyl-tRNA synthetase
ScanRegExp IPR001412 92 103 PS00178 Aminoacyl-tRNA synthetase
Expression profile
Description

Northern blot
Experimental conditions
Primer_f
Primer_r
PCR conditions test

RH mapping information
Description

Chromosome No. 3
Experimental conditions
Panel name Genebridge 4
Primer_f GGGAAGCCATCAGAGACACT
Primer_r CTGAAGGCAAAGAGACCATT
PCR product length 216 bp
PCR conditions 95 °C15 sec60 °C60 sec30 cycles
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