Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC021547A_C01 KMC021547A_c01
(480 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_199729.1| pectinesterase family; protein id: At5g49180.1,... 80 1e-14
gb|AAF63815.1| pectin methylesterase, putative [Arabidopsis thal... 75 4e-13
ref|NP_187339.2| pectin methylesterase, putative; protein id: At... 75 4e-13
emb|CAB65290.2| pectin methyl-esterase PER [Medicago truncatula] 74 8e-13
emb|CAB65291.1| pectin methyl-esterase PEF1 [Medicago truncatula] 69 3e-11
>ref|NP_199729.1| pectinesterase family; protein id: At5g49180.1, supported by cDNA:
6699., supported by cDNA: gi_18700150 [Arabidopsis
thaliana] gi|10177147|dbj|BAB10336.1| pectin
methylesterase [Arabidopsis thaliana]
gi|18700151|gb|AAL77687.1| AT5g49180/K21P3_5
[Arabidopsis thaliana] gi|21594190|gb|AAM65978.1| pectin
methylesterase [Arabidopsis thaliana]
gi|25090082|gb|AAN72223.1| At5g49180/K21P3_5
[Arabidopsis thaliana]
Length = 571
Score = 80.1 bits (196), Expect = 1e-14
Identities = 40/85 (47%), Positives = 58/85 (68%)
Frame = -3
Query: 478 IGDLITPDGYMPCQLMDGTLAGMDTCYYAEFNNRGPGSDQSKRVKWPGVKTLTSDVAISF 299
I D+I P G++P +G A ++T YYAE+ N GPGS+Q++RVKWPG+K L+ A+ F
Sbjct: 492 IDDVIDPAGWLP---WNGDFA-LNTLYYAEYENNGPGSNQAQRVKWPGIKKLSPKQALRF 547
Query: 298 SPSRFFRGDDWIKVTRVTLQPWQGH 224
+P+RF RG+ WI RV P+ G+
Sbjct: 548 TPARFLRGNLWIPPNRV---PYMGN 569
>gb|AAF63815.1| pectin methylesterase, putative [Arabidopsis thaliana]
Length = 562
Score = 75.1 bits (183), Expect = 4e-13
Identities = 38/77 (49%), Positives = 50/77 (64%)
Frame = -3
Query: 478 IGDLITPDGYMPCQLMDGTLAGMDTCYYAEFNNRGPGSDQSKRVKWPGVKTLTSDVAISF 299
I D+I P G++P G A + T YYAE N GPGS+Q++RVKWPG+K LT A+ +
Sbjct: 484 IDDVIDPAGWLP---WSGDFA-LKTLYYAEHMNTGPGSNQAQRVKWPGIKKLTPQDALLY 539
Query: 298 SPSRFFRGDDWIKVTRV 248
+ RF RGD WI T+V
Sbjct: 540 TGDRFLRGDTWIPQTQV 556
>ref|NP_187339.2| pectin methylesterase, putative; protein id: At3g06830.1
[Arabidopsis thaliana] gi|26451728|dbj|BAC42959.1|
putative pectin methylesterase [Arabidopsis thaliana]
Length = 568
Score = 75.1 bits (183), Expect = 4e-13
Identities = 38/77 (49%), Positives = 50/77 (64%)
Frame = -3
Query: 478 IGDLITPDGYMPCQLMDGTLAGMDTCYYAEFNNRGPGSDQSKRVKWPGVKTLTSDVAISF 299
I D+I P G++P G A + T YYAE N GPGS+Q++RVKWPG+K LT A+ +
Sbjct: 490 IDDVIDPAGWLP---WSGDFA-LKTLYYAEHMNTGPGSNQAQRVKWPGIKKLTPQDALLY 545
Query: 298 SPSRFFRGDDWIKVTRV 248
+ RF RGD WI T+V
Sbjct: 546 TGDRFLRGDTWIPQTQV 562
>emb|CAB65290.2| pectin methyl-esterase PER [Medicago truncatula]
Length = 602
Score = 73.9 bits (180), Expect = 8e-13
Identities = 37/78 (47%), Positives = 48/78 (61%), Gaps = 6/78 (7%)
Frame = -3
Query: 478 IGDLITPDGYMPCQLMDGTLAGMDTCYYAEFNNRGPGSDQSKRVKWPGVKTLTSDVAISF 299
I D+ P+GYMP A DTC + E+NN+GPG+D SKRVKWPGVK+++S A +F
Sbjct: 462 IDDIFDPEGYMPWM----GSAFKDTCTFYEYNNKGPGADTSKRVKWPGVKSISSTEAAAF 517
Query: 298 SPSRFFR------GDDWI 263
P +FF D WI
Sbjct: 518 YPGKFFEIANATDRDTWI 535
>emb|CAB65291.1| pectin methyl-esterase PEF1 [Medicago truncatula]
Length = 565
Score = 68.6 bits (166), Expect = 3e-11
Identities = 36/78 (46%), Positives = 48/78 (61%), Gaps = 6/78 (7%)
Frame = -3
Query: 478 IGDLITPDGYMPCQLMDGTLAGMDTCYYAEFNNRGPGSDQSKRVKWPGVKTLTSDVAISF 299
I + P+GYMP ++ G A DTC + E+NN+GPG+D + RVKW GVK LTS+VA +
Sbjct: 480 IDAMFAPEGYMP--MVGG--AFKDTCTFYEYNNKGPGADTNLRVKWHGVKVLTSNVAAEY 535
Query: 298 SPSRFFR------GDDWI 263
P +FF D WI
Sbjct: 536 YPGKFFEIVNATARDTWI 553
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 424,352,856
Number of Sequences: 1393205
Number of extensions: 8611214
Number of successful extensions: 18968
Number of sequences better than 10.0: 158
Number of HSP's better than 10.0 without gapping: 18547
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 18937
length of database: 448,689,247
effective HSP length: 113
effective length of database: 291,257,082
effective search space used: 13397825772
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
