KMC020521A_c01
[Fasta Sequence]   [Nr Search]   [EST assemble image]  

Fasta Sequence
>KMC020521A_C01 KMC020521A_c01
GCTGAAATTCAAACTTTATTGAACTTGGATACCAAATATTACAATTATAATACAACCGTC
ACACTCTTAGCTTGTGAAAATTGAACTATATATTACAATTTGATACCAAAATTTTAAATA
GAATCATCATTTTCATTTTACTATATTCATCGATGTCCTTTCAAAAAAACTATATTCATC
AATGTATGTGCAATGAACATGCTTATGTAGTTCAGTTTTCGATGGTGTTCTTGCGGTTAT
CATTGTTGTCAGCTGCAGGAACAATCTTTGACCAGAACCAATGTGACCTCCACACACTGT
TCATCTCTTCAATTGGGACTCCCTTGGTCTCAGGCAAAAACAATGCAACAAAGATGGTCA
TGATGACCACAAACCCAGCAAAGAAGAAGAAGAGTCCAAATTTCAAGTGGCAGAGCATGG
AGAGGAAAATCTGAGCAATGGCGAAGGTGAACAACATGTTAACAGCAACATTGAGGGCTT
GACCAGCAGATCGAACCTCGAGAGAGCAGATTTCA


Nr search

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC020521A_C01 KMC020521A_c01
         (515 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gb|AAC61852.1| putative monosaccharide transporter 1 [Petunia x ...   142  2e-33
ref|NP_193879.1| glucose transporter; protein id: At4g21480.1 [A...   142  2e-33
ref|NP_188627.1| monosaccharide transport protein, STP4; protein...   142  3e-33
dbj|BAB19864.1| monosaccharide transporter 3 [Oryza sativa] gi|2...   139  1e-32
emb|CAB52688.1| hexose transporter [Lycopersicon esculentum]          139  2e-32

>gb|AAC61852.1| putative monosaccharide transporter 1 [Petunia x hybrida]
          Length = 510

 Score =  142 bits (358), Expect = 2e-33
 Identities = 59/98 (60%), Positives = 80/98 (81%)
 Frame = -2

Query: 514 EICSLEVRSAGQALNVAVNMLFTFAIAQIFLSMLCHLKFGLFFFFAGFVVIMTIFVALFL 335
           E+  +E+RSAGQ++ V+VNM FTF I Q+FL+MLC +KFGLFFFFAGFVV+MT+F+  FL
Sbjct: 413 EVFPMEIRSAGQSITVSVNMFFTFVIGQLFLTMLCEMKFGLFFFFAGFVVLMTLFIFFFL 472

Query: 334 PETKGVPIEEMNSVWRSHWFWSKIVPAADNNDNRKNTI 221
           PETKG+PIEE+N +W++HWFW   VP  D++ + KN +
Sbjct: 473 PETKGIPIEEVNRIWKNHWFWKSYVPNDDDDHHSKNQV 510

>ref|NP_193879.1| glucose transporter; protein id: At4g21480.1 [Arabidopsis thaliana]
           gi|7446744|pir||T05156 probable glucose transport
           protein F18E5.100 - Arabidopsis thaliana
           gi|3080392|emb|CAA18712.1| glucose transporter
           [Arabidopsis thaliana] gi|7268945|emb|CAB81255.1|
           glucose transporter [Arabidopsis thaliana]
           gi|15487248|emb|CAC69069.1| STP12 protein [Arabidopsis
           thaliana]
          Length = 508

 Score =  142 bits (358), Expect = 2e-33
 Identities = 66/90 (73%), Positives = 76/90 (84%)
 Frame = -2

Query: 514 EICSLEVRSAGQALNVAVNMLFTFAIAQIFLSMLCHLKFGLFFFFAGFVVIMTIFVALFL 335
           EI  LE+RSA Q++ V+VNM+FTF IAQ+FL MLCHLKFGLF FFA FVV+M+IFV LFL
Sbjct: 413 EIFPLEIRSAAQSITVSVNMIFTFLIAQVFLMMLCHLKFGLFIFFAFFVVVMSIFVYLFL 472

Query: 334 PETKGVPIEEMNSVWRSHWFWSKIVPAADN 245
           PET+GVPIEEMN VWRSHW+WSK V A  N
Sbjct: 473 PETRGVPIEEMNRVWRSHWYWSKFVDAEKN 502

>ref|NP_188627.1| monosaccharide transport protein, STP4; protein id: At3g19930.1,
           supported by cDNA: gi_13605905, supported by cDNA:
           gi_16226823 [Arabidopsis thaliana] gi|99758|pir||S25009
           monosaccharide transport protein STP4 - Arabidopsis
           thaliana gi|16524|emb|CAA47325.1| sugar transport
           protein [Arabidopsis thaliana]
           gi|11994205|dbj|BAB01308.1| monosaccharide transporter
           STP4 [Arabidopsis thaliana]
           gi|13605906|gb|AAK32938.1|AF367352_1 AT3g19930/MPN9_17
           [Arabidopsis thaliana]
           gi|16226824|gb|AAL16272.1|AF428342_1 AT3g19930/MPN9_17
           [Arabidopsis thaliana] gi|22137154|gb|AAM91422.1|
           AT3g19930/MPN9_17 [Arabidopsis thaliana]
          Length = 514

 Score =  142 bits (357), Expect = 3e-33
 Identities = 63/86 (73%), Positives = 74/86 (85%)
 Frame = -2

Query: 514 EICSLEVRSAGQALNVAVNMLFTFAIAQIFLSMLCHLKFGLFFFFAGFVVIMTIFVALFL 335
           EI  LE+RSA QA+NV+VNM FTF +AQ+FL+MLCH+KFGLFFFFA FVVIMTIF+ L L
Sbjct: 413 EISPLEIRSAAQAINVSVNMFFTFLVAQLFLTMLCHMKFGLFFFFAFFVVIMTIFIYLML 472

Query: 334 PETKGVPIEEMNSVWRSHWFWSKIVP 257
           PETK VPIEEMN VW++HWFW K +P
Sbjct: 473 PETKNVPIEEMNRVWKAHWFWGKFIP 498

>dbj|BAB19864.1| monosaccharide transporter 3 [Oryza sativa]
           gi|22324466|dbj|BAC10381.1| monosaccharide transporter 3
           [Oryza sativa (japonica cultivar-group)]
          Length = 518

 Score =  139 bits (351), Expect = 1e-32
 Identities = 62/85 (72%), Positives = 75/85 (87%)
 Frame = -2

Query: 514 EICSLEVRSAGQALNVAVNMLFTFAIAQIFLSMLCHLKFGLFFFFAGFVVIMTIFVALFL 335
           EI  LE+R AGQ++NV+VNMLFTF IAQ FL+MLCH+KFGLF+FFAG+VVIMT+F+ALFL
Sbjct: 415 EIFPLEIRPAGQSINVSVNMLFTFVIAQAFLTMLCHMKFGLFYFFAGWVVIMTVFIALFL 474

Query: 334 PETKGVPIEEMNSVWRSHWFWSKIV 260
           PETK VPIEEM  VW+SHWFW + +
Sbjct: 475 PETKNVPIEEMVLVWKSHWFWRRFI 499

>emb|CAB52688.1| hexose transporter [Lycopersicon esculentum]
          Length = 292

 Score =  139 bits (350), Expect = 2e-32
 Identities = 63/97 (64%), Positives = 76/97 (77%)
 Frame = -2

Query: 514 EICSLEVRSAGQALNVAVNMLFTFAIAQIFLSMLCHLKFGLFFFFAGFVVIMTIFVALFL 335
           EI  LE+RSA Q++NV+VNM+FTFA+AQ+FL+MLCHLKFGLF FFA FVVIMT+F+  FL
Sbjct: 182 EIFPLEIRSAAQSINVSVNMIFTFAVAQVFLTMLCHLKFGLFLFFAFFVVIMTVFIYFFL 241

Query: 334 PETKGVPIEEMNSVWRSHWFWSKIVPAADNNDNRKNT 224
           PETK +PIEEM  VW+ HWFWSK +   D    R  T
Sbjct: 242 PETKNIPIEEMVIVWKEHWFWSKFMTEVDYPGTRNGT 278

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 434,699,955
Number of Sequences: 1393205
Number of extensions: 9582809
Number of successful extensions: 32218
Number of sequences better than 10.0: 451
Number of HSP's better than 10.0 without gapping: 30079
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 31966
length of database: 448,689,247
effective HSP length: 115
effective length of database: 288,470,672
effective search space used: 16154357632
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)


EST assemble image


clone accession position
1 MF017a06_f BP029124 1 457
2 MF025b07_f BP029568 1 517
3 MF025h08_f BP029606 3 402
4 MF031b03_f BP029892 3 312
5 MF004h05_f BP028467 32 182




Lotus japonicus
Kazusa DNA Research Institute