Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC019179A_C01 KMC019179A_c01
(558 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|P26205|BGLT_TRIRP Cyanogenic beta-glucosidase precursor (Lina... 265 4e-70
pdb|1CBG| Cyanogenic Beta-Glucosidase Mol_id: 1; Molecule: Cya... 265 4e-70
sp|P26204|BGLS_TRIRP NON-CYANOGENIC BETA-GLUCOSIDASE PRECURSOR g... 263 1e-69
gb|AAA93234.2|AAA93234 amygdalin hydrolase isoform AH I precurso... 259 2e-68
gb|AAL07489.1|AF414606_1 amygdalin hydrolase isoform AH I precur... 258 3e-68
>sp|P26205|BGLT_TRIRP Cyanogenic beta-glucosidase precursor (Linamarase)
gi|67490|pir||GLJY14 beta-glucosidase (EC 3.2.1.21)
precursor (clone TRE104) - white clover (fragment)
gi|21953|emb|CAA40057.1| beta-glucosidase [Trifolium
repens]
Length = 425
Score = 265 bits (676), Expect = 4e-70
Identities = 119/159 (74%), Positives = 138/159 (85%)
Frame = +1
Query: 82 DVASLNRSSFPKGFIFGTASSAYQYEGAANKYGRKPSIWDTYAHNYSERIVDRSNGDVAV 261
D + LNRS F GF+FGTASSA+QYEGAA + G+ PSIWDT+ H Y E+I DR+NGDVA+
Sbjct: 21 DFSDLNRSCFAPGFVFGTASSAFQYEGAAFEDGKGPSIWDTFTHKYPEKIKDRTNGDVAI 80
Query: 262 DEYHRYKEDVGIMKSMNMDAYSFSISWSRILPKGKLRGGINQEGIKYYNNLINELLANGL 441
DEYHRYKED+GIMK MN+DAY FSISW R+LPKGKL GG+N+EGI YYNNLINE+LANG+
Sbjct: 81 DEYHRYKEDIGIMKDMNLDAYRFSISWPRVLPKGKLSGGVNREGINYYNNLINEVLANGM 140
Query: 442 QPYVTLFHWDMPQALEDEYGGFLSPHVVKDFRDYAELCF 558
QPYVTLFHWD+PQALEDEY GFL ++V DFRDYAELCF
Sbjct: 141 QPYVTLFHWDVPQALEDEYRGFLGRNIVDDFRDYAELCF 179
>pdb|1CBG| Cyanogenic Beta-Glucosidase Mol_id: 1; Molecule: Cyanogenic
Beta-Glucosidase; Chain: Null; Ec: 3.2.1.21
Length = 490
Score = 265 bits (676), Expect = 4e-70
Identities = 119/159 (74%), Positives = 138/159 (85%)
Frame = +1
Query: 82 DVASLNRSSFPKGFIFGTASSAYQYEGAANKYGRKPSIWDTYAHNYSERIVDRSNGDVAV 261
D + LNRS F GF+FGTASSA+QYEGAA + G+ PSIWDT+ H Y E+I DR+NGDVA+
Sbjct: 10 DFSDLNRSCFAPGFVFGTASSAFQYEGAAFEDGKGPSIWDTFTHKYPEKIKDRTNGDVAI 69
Query: 262 DEYHRYKEDVGIMKSMNMDAYSFSISWSRILPKGKLRGGINQEGIKYYNNLINELLANGL 441
DEYHRYKED+GIMK MN+DAY FSISW R+LPKGKL GG+N+EGI YYNNLINE+LANG+
Sbjct: 70 DEYHRYKEDIGIMKDMNLDAYRFSISWPRVLPKGKLSGGVNREGINYYNNLINEVLANGM 129
Query: 442 QPYVTLFHWDMPQALEDEYGGFLSPHVVKDFRDYAELCF 558
QPYVTLFHWD+PQALEDEY GFL ++V DFRDYAELCF
Sbjct: 130 QPYVTLFHWDVPQALEDEYRGFLGRNIVDDFRDYAELCF 168
>sp|P26204|BGLS_TRIRP NON-CYANOGENIC BETA-GLUCOSIDASE PRECURSOR gi|67491|pir||GLJY31
beta-glucosidase (EC 3.2.1.21) precursor (clone TRE361)
- white clover gi|21955|emb|CAA40058.1| beta-glucosidase
[Trifolium repens]
Length = 493
Score = 263 bits (672), Expect = 1e-69
Identities = 118/159 (74%), Positives = 135/159 (84%)
Frame = +1
Query: 82 DVASLNRSSFPKGFIFGTASSAYQYEGAANKYGRKPSIWDTYAHNYSERIVDRSNGDVAV 261
D+ +L+RSSFP+GFIFG SSAYQ+EGA N+ GR PSIWDT+ H Y E+I D SN D+ V
Sbjct: 31 DIGNLSRSSFPRGFIFGAGSSAYQFEGAVNEGGRGPSIWDTFTHKYPEKIRDGSNADITV 90
Query: 262 DEYHRYKEDVGIMKSMNMDAYSFSISWSRILPKGKLRGGINQEGIKYYNNLINELLANGL 441
D+YHRYKEDVGIMK NMD+Y FSISW RILPKGKL GGIN EGIKYYNNLINELLANG+
Sbjct: 91 DQYHRYKEDVGIMKDQNMDSYRFSISWPRILPKGKLSGGINHEGIKYYNNLINELLANGI 150
Query: 442 QPYVTLFHWDMPQALEDEYGGFLSPHVVKDFRDYAELCF 558
QP+VTLFHWD+PQ LEDEYGGFL+ V+ DFRDY +LCF
Sbjct: 151 QPFVTLFHWDLPQVLEDEYGGFLNSGVINDFRDYTDLCF 189
>gb|AAA93234.2|AAA93234 amygdalin hydrolase isoform AH I precursor [Prunus serotina]
Length = 553
Score = 259 bits (661), Expect = 2e-68
Identities = 121/164 (73%), Positives = 140/164 (84%), Gaps = 3/164 (1%)
Frame = +1
Query: 76 HCDVASLNRSSFPK---GFIFGTASSAYQYEGAANKYGRKPSIWDTYAHNYSERIVDRSN 246
HC ASLNRSSF GFIFGTAS+AYQ+EGAA + GR PSIWDTY HN+SERI D SN
Sbjct: 33 HC--ASLNRSSFDALEPGFIFGTASAAYQFEGAAKEDGRGPSIWDTYTHNHSERIKDGSN 90
Query: 247 GDVAVDEYHRYKEDVGIMKSMNMDAYSFSISWSRILPKGKLRGGINQEGIKYYNNLINEL 426
GDVAVD+YHRYKEDV IMK M DAY FSISWSR+LP GK+ GG+N++GIK+YNNLINE+
Sbjct: 91 GDVAVDQYHRYKEDVRIMKKMGFDAYRFSISWSRVLPNGKISGGVNEDGIKFYNNLINEI 150
Query: 427 LANGLQPYVTLFHWDMPQALEDEYGGFLSPHVVKDFRDYAELCF 558
L NGL+P+VT++HWD+PQALEDEYGGFLSP++V FRDYA LCF
Sbjct: 151 LRNGLKPFVTIYHWDLPQALEDEYGGFLSPNIVDHFRDYANLCF 194
>gb|AAL07489.1|AF414606_1 amygdalin hydrolase isoform AH I precursor [Prunus serotina]
Length = 528
Score = 258 bits (660), Expect = 3e-68
Identities = 121/164 (73%), Positives = 140/164 (84%), Gaps = 3/164 (1%)
Frame = +1
Query: 76 HCDVASLNRSSFPK---GFIFGTASSAYQYEGAANKYGRKPSIWDTYAHNYSERIVDRSN 246
HC ASLNRSSF GFIFGTAS+AYQ+EGAA + GR PSIWDTY HN+SERI D SN
Sbjct: 8 HC--ASLNRSSFDALEPGFIFGTASAAYQFEGAAKEDGRGPSIWDTYTHNHSERIKDGSN 65
Query: 247 GDVAVDEYHRYKEDVGIMKSMNMDAYSFSISWSRILPKGKLRGGINQEGIKYYNNLINEL 426
GDVAVD+YHRYKEDV IMK M DAY FSISWSR+LP GK+ GG+N++GIK+YNNLINE+
Sbjct: 66 GDVAVDQYHRYKEDVRIMKKMGFDAYRFSISWSRVLPNGKVSGGVNEDGIKFYNNLINEI 125
Query: 427 LANGLQPYVTLFHWDMPQALEDEYGGFLSPHVVKDFRDYAELCF 558
L NGL+P+VT++HWD+PQALEDEYGGFLSP++V FRDYA LCF
Sbjct: 126 LRNGLKPFVTIYHWDLPQALEDEYGGFLSPNIVDHFRDYANLCF 169
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 472,883,412
Number of Sequences: 1393205
Number of extensions: 10119691
Number of successful extensions: 28080
Number of sequences better than 10.0: 534
Number of HSP's better than 10.0 without gapping: 26510
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 27235
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 19808345223
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
