KMC018034A_c01
[Fasta Sequence]   [Nr Search]   [EST assemble image]  

Fasta Sequence
>KMC018034A_C01 KMC018034A_c01
GATCCAAAAGTGCTAAGCCAAAATATTATTAAGAGACAGGAAAAAGTCATGCCTTCAAGA
CAAGTGCTGGTATTTTCAAAAGGATAATGCATCCTTAAAAAATGGACAATAATTATTGAT
AGTAATATACATGCTTAGCCATTTTGACAAAACAAATAAAAATATTGATGACACATAGCA
CTCGCATAACCATAAATGAAATGGAACATGGAGCTGAAAGGGAGGACAAAGCAGGAGCCA
ATTACAATGACAATGGAGTTTTTCTTTTACTCGCCCCTCTCCTCTTTTTTTCTTTTATCC
ATCCAATCAACACAAATAAGTATATATATTTAGGACACATAGATCTGATAAAAACCAAGC
AAGGCGAGAGGTAAATTTCCAATCTACACCAGATGATGCCACTCCAGCGATCGATCAACC
GGCCAATATATTCAGGCACTTTCTCCTGGAACTCGATTCAAAGCATCAAAGTTGTTCACA
GGTTTGGGCTTCCACTCAGTCCTACCCCTCACAATCTCAGCACCATCATCAAGTCGA


Nr search

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC018034A_C01 KMC018034A_c01
         (537 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gb|AAB51525.1| acyl-ACP thioesterase [Garcinia mangostana]             52  5e-06
gb|AAD01982.1| palmitoyl-acyl carrier protein thioesterase [Goss...    40  0.014
sp|Q9SQI3|FATB_GOSHI Myristoyl-acyl carrier protein thioesterase...    40  0.014
gb|AAB71730.1| acyl-ACP thioesterase [Myristica fragrans]              39  0.040
gb|AAC49001.1| Uc FatB2                                                39  0.052

>gb|AAB51525.1| acyl-ACP thioesterase [Garcinia mangostana]
          Length = 406

 Score = 52.0 bits (123), Expect = 5e-06
 Identities = 20/34 (58%), Positives = 29/34 (84%)
 Frame = -2

Query: 536 RLDDGAEIVRGRTEWKPKPVNNFDALNRVPGESA 435
           RL+DGAEIVRGRTEW+PK +NNF  + ++P +++
Sbjct: 373 RLEDGAEIVRGRTEWRPKYINNFSIMGQIPTDAS 406

>gb|AAD01982.1| palmitoyl-acyl carrier protein thioesterase [Gossypium hirsutum]
          Length = 410

 Score = 40.4 bits (93), Expect = 0.014
 Identities = 17/34 (50%), Positives = 24/34 (70%)
 Frame = -2

Query: 536 RLDDGAEIVRGRTEWKPKPVNNFDALNRVPGESA 435
           RLDDGAEIVRGRT W+PK   +   ++++  + A
Sbjct: 377 RLDDGAEIVRGRTRWRPKHAKSSANMDQITAKRA 410

>sp|Q9SQI3|FATB_GOSHI Myristoyl-acyl carrier protein thioesterase, chloroplast precursor
           (16:0-acyl-carrier protein thioesterase) (16:0-ACP
           thioesterase) (Acyl-[acyl-carrier protein] hydrolase)
           (PATE) gi|6048397|gb|AAF02215.1|AF076535_1
           palmitoyl-acyl carrier protein thioesterase [Gossypium
           hirsutum]
          Length = 413

 Score = 40.4 bits (93), Expect = 0.014
 Identities = 17/34 (50%), Positives = 24/34 (70%)
 Frame = -2

Query: 536 RLDDGAEIVRGRTEWKPKPVNNFDALNRVPGESA 435
           RLDDGAEIVRGRT W+PK   +   ++++  + A
Sbjct: 380 RLDDGAEIVRGRTRWRPKHAKSSANMDQITAKRA 413

>gb|AAB71730.1| acyl-ACP thioesterase [Myristica fragrans]
          Length = 362

 Score = 38.9 bits (89), Expect = 0.040
 Identities = 18/40 (45%), Positives = 27/40 (67%), Gaps = 3/40 (7%)
 Frame = -2

Query: 536 RLDDGAEIVRGRTEWKPKPVNN---FDALNRVPGESA*IY 426
           RL+DG+EI+RG+TEW+PK   N   F +++ +P     IY
Sbjct: 296 RLEDGSEIMRGKTEWRPKRAANTTYFGSVDDIPPHPIYIY 335

>gb|AAC49001.1| Uc FatB2
          Length = 383

 Score = 38.5 bits (88), Expect = 0.052
 Identities = 12/27 (44%), Positives = 24/27 (88%)
 Frame = -2

Query: 536 RLDDGAEIVRGRTEWKPKPVNNFDALN 456
           +L+DG+E++RGRT+W+PK  ++F+ ++
Sbjct: 347 QLEDGSEVLRGRTDWRPKRTDSFEGIS 373

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 473,132,070
Number of Sequences: 1393205
Number of extensions: 10516265
Number of successful extensions: 34692
Number of sequences better than 10.0: 43
Number of HSP's better than 10.0 without gapping: 33716
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 34683
length of database: 448,689,247
effective HSP length: 115
effective length of database: 288,470,672
effective search space used: 18173652336
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)


EST assemble image


clone accession position
1 MFB019d08_f BP035339 1 473
2 MF045f02_f BP030677 1 524
3 MF016a11_f BP029072 51 248
4 SPD015f04_f BP045199 54 538
5 MF041h12_f BP030468 56 528
6 MF099e10_f BP033458 69 405
7 MF066a07_f BP031790 69 493
8 MF033h11_f BP030061 94 540
9 MF072h02_f BP032141 110 218
10 MFBL013a09_f BP041898 149 314
11 MFBL034a07_f BP042946 150 473




Lotus japonicus
Kazusa DNA Research Institute