Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC017960A_C01 KMC017960A_c01
(624 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|O23787|THI4_CITSI Thiazole biosynthetic enzyme, chloroplast p... 183 3e-67
dbj|BAA88227.1| thiamin biosynthetic enzyme [Glycine max] 172 6e-63
dbj|BAC45141.1| putative thiamine biosynthetic enzyme [Oryza sat... 169 1e-62
sp|Q41738|TH41_MAIZE Thiazole biosynthetic enzyme 1-1, chloropla... 172 1e-62
dbj|BAA88225.1| thiamin biosynthetic enzyme [Glycine max] 172 1e-62
>sp|O23787|THI4_CITSI Thiazole biosynthetic enzyme, chloroplast precursor
gi|7446894|pir||T10474 thiamin biosynthesis protein thi1
- sweet orange gi|2582665|emb|CAB05370.1| thi [Citrus
sinensis]
Length = 356
Score = 183 bits (465), Expect(2) = 3e-67
Identities = 98/142 (69%), Positives = 109/142 (76%), Gaps = 2/142 (1%)
Frame = +2
Query: 53 MAAMATTTLTSSLISNPKSSF-SSFHGKPISTRTTSIKSTTQQNTTTISMSLN-SPAYDL 226
MAAMA+T S+ S F SSFHG P+S ++ +S+S + SP YDL
Sbjct: 1 MAAMASTAFAPSVSSTTNKLFDSSFHGAPMSPSLLRLQPIKSSRPNNLSISASASPPYDL 60
Query: 227 EAFKFAPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKDPSISIAII 406
FKF PIKESIVSREMTRRYMTDMITYADTDVV+VGAGSAGLSCAYELSK+P+I IAII
Sbjct: 61 NTFKFDPIKESIVSREMTRRYMTDMITYADTDVVVVGAGSAGLSCAYELSKNPNIQIAII 120
Query: 407 EQSVSPGGGAWLGGQLFSAMVV 472
EQSVSPGGGAWLGGQLFSAMVV
Sbjct: 121 EQSVSPGGGAWLGGQLFSAMVV 142
Score = 94.0 bits (232), Expect(2) = 3e-67
Identities = 43/50 (86%), Positives = 50/50 (100%)
Frame = +3
Query: 474 RKPAHLFLNEIGVDYDEQEDYVVIKHAALFTSTIMSKLLAKPNVKLFNAV 623
RKPAH+FL+E+G+DYDEQ++YVVIKHAALFTSTIMSKLLA+PNVKLFNAV
Sbjct: 143 RKPAHIFLDELGIDYDEQDNYVVIKHAALFTSTIMSKLLARPNVKLFNAV 192
>dbj|BAA88227.1| thiamin biosynthetic enzyme [Glycine max]
Length = 345
Score = 172 bits (435), Expect(2) = 6e-63
Identities = 92/143 (64%), Positives = 109/143 (75%), Gaps = 6/143 (4%)
Frame = +2
Query: 62 MATTTLTSSLISNP------KSSFSSFHGKPISTRTTSIKSTTQQNTTTISMSLNSPAYD 223
MA++T+TSS +++P KSS SFH P T + SMS ++P YD
Sbjct: 1 MASSTITSSFLTSPPSSLFNKSSSPSFHATP----------TLRPLAPRASMSASAPPYD 50
Query: 224 LEAFKFAPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKDPSISIAI 403
+F+F PI+ESIVSREMTRRYM DM+T+ADTDVVIVGAGSAGLSCAYELSK+PSI+IAI
Sbjct: 51 FGSFRFDPIRESIVSREMTRRYMIDMVTHADTDVVIVGAGSAGLSCAYELSKNPSINIAI 110
Query: 404 IEQSVSPGGGAWLGGQLFSAMVV 472
+EQSVSPGGGAWLGGQLFSAMVV
Sbjct: 111 VEQSVSPGGGAWLGGQLFSAMVV 133
Score = 91.3 bits (225), Expect(2) = 6e-63
Identities = 43/50 (86%), Positives = 49/50 (98%)
Frame = +3
Query: 474 RKPAHLFLNEIGVDYDEQEDYVVIKHAALFTSTIMSKLLAKPNVKLFNAV 623
RKPAHLFL+E+ V+YDEQ++YVVIKHAALFTSTIMSKLLA+PNVKLFNAV
Sbjct: 134 RKPAHLFLDELNVEYDEQDNYVVIKHAALFTSTIMSKLLARPNVKLFNAV 183
>dbj|BAC45141.1| putative thiamine biosynthetic enzyme [Oryza sativa (japonica
cultivar-group)]
Length = 352
Score = 169 bits (427), Expect(2) = 1e-62
Identities = 93/140 (66%), Positives = 109/140 (77%), Gaps = 3/140 (2%)
Frame = +2
Query: 62 MATTTLTSSLISNPKSSFSSFHGKPISTRTTSIKSTTQQN---TTTISMSLNSPAYDLEA 232
MATT SSL+ K+SF+ P + R ++ + +IS S ++P YDL A
Sbjct: 1 MATTA--SSLL---KTSFAGAR-LPAAARNPTVSVAPRTGGAICNSISSSSSTPPYDLNA 54
Query: 233 FKFAPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKDPSISIAIIEQ 412
+F+PIKESIVSREMTRRYMTDMITYADTDVV+VGAGSAGLSCAYELSKDPS+S+A+IEQ
Sbjct: 55 IRFSPIKESIVSREMTRRYMTDMITYADTDVVVVGAGSAGLSCAYELSKDPSVSVAVIEQ 114
Query: 413 SVSPGGGAWLGGQLFSAMVV 472
SVSPGGGAWLGGQLFSAMVV
Sbjct: 115 SVSPGGGAWLGGQLFSAMVV 134
Score = 93.6 bits (231), Expect(2) = 1e-62
Identities = 44/50 (88%), Positives = 49/50 (98%)
Frame = +3
Query: 474 RKPAHLFLNEIGVDYDEQEDYVVIKHAALFTSTIMSKLLAKPNVKLFNAV 623
RKPAHLFL+E+GV YDEQEDYVVIKHAALFTST+MS+LLA+PNVKLFNAV
Sbjct: 135 RKPAHLFLDELGVAYDEQEDYVVIKHAALFTSTVMSRLLARPNVKLFNAV 184
>sp|Q41738|TH41_MAIZE Thiazole biosynthetic enzyme 1-1, chloroplast precursor
gi|2130146|pir||S61419 thiamin biosynthesis protein
thi1-1 - maize gi|596078|gb|AAA96738.1| thiamine
biosynthetic enzyme
Length = 354
Score = 172 bits (436), Expect(2) = 1e-62
Identities = 96/146 (65%), Positives = 109/146 (73%), Gaps = 9/146 (6%)
Frame = +2
Query: 62 MATTTLTSSLISNPKSSFSSFHGKPISTRTTSIKSTTQQNT---------TTISMSLNSP 214
MAT +S L KSSF+ P +TRTT ++SMS ++P
Sbjct: 1 MATAAASSLL----KSSFAGSR-LPAATRTTPASLVVATGPRGAGAGPICASMSMSSSNP 55
Query: 215 AYDLEAFKFAPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKDPSIS 394
YDL +F+F+PIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKDP++S
Sbjct: 56 PYDLTSFRFSPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKDPAVS 115
Query: 395 IAIIEQSVSPGGGAWLGGQLFSAMVV 472
IAI+EQSVSPGGGAWLGGQLFSAMVV
Sbjct: 116 IAIVEQSVSPGGGAWLGGQLFSAMVV 141
Score = 89.7 bits (221), Expect(2) = 1e-62
Identities = 43/50 (86%), Positives = 47/50 (94%)
Frame = +3
Query: 474 RKPAHLFLNEIGVDYDEQEDYVVIKHAALFTSTIMSKLLAKPNVKLFNAV 623
RKPAHLFL+E+GV YDE EDYVVIKHAALFTST+MS LLA+PNVKLFNAV
Sbjct: 142 RKPAHLFLDELGVAYDEAEDYVVIKHAALFTSTVMSLLLARPNVKLFNAV 191
>dbj|BAA88225.1| thiamin biosynthetic enzyme [Glycine max]
Length = 345
Score = 172 bits (435), Expect(2) = 1e-62
Identities = 92/143 (64%), Positives = 109/143 (75%), Gaps = 6/143 (4%)
Frame = +2
Query: 62 MATTTLTSSLISNP------KSSFSSFHGKPISTRTTSIKSTTQQNTTTISMSLNSPAYD 223
MA++T+TSS +++P KSS SFH P T + SMS ++P YD
Sbjct: 1 MASSTITSSFLTSPPSSLFNKSSSPSFHATP----------TLRPLAPRASMSASAPPYD 50
Query: 224 LEAFKFAPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKDPSISIAI 403
+F+F PI+ESIVSREMTRRYM DM+T+ADTDVVIVGAGSAGLSCAYELSK+PSI+IAI
Sbjct: 51 FGSFRFDPIRESIVSREMTRRYMIDMVTHADTDVVIVGAGSAGLSCAYELSKNPSINIAI 110
Query: 404 IEQSVSPGGGAWLGGQLFSAMVV 472
+EQSVSPGGGAWLGGQLFSAMVV
Sbjct: 111 VEQSVSPGGGAWLGGQLFSAMVV 133
Score = 90.1 bits (222), Expect(2) = 1e-62
Identities = 42/50 (84%), Positives = 49/50 (98%)
Frame = +3
Query: 474 RKPAHLFLNEIGVDYDEQEDYVVIKHAALFTSTIMSKLLAKPNVKLFNAV 623
RKPAHLFL+E+ ++YDEQ++YVVIKHAALFTSTIMSKLLA+PNVKLFNAV
Sbjct: 134 RKPAHLFLDELNLEYDEQDNYVVIKHAALFTSTIMSKLLARPNVKLFNAV 183
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 634,829,491
Number of Sequences: 1393205
Number of extensions: 18860542
Number of successful extensions: 467218
Number of sequences better than 10.0: 10987
Number of HSP's better than 10.0 without gapping: 114593
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 271163
length of database: 448,689,247
effective HSP length: 118
effective length of database: 284,291,057
effective search space used: 25301904073
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
