Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC017406A_C01 KMC017406A_c01
(713 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_565693.1| ubiquitin activating enzyme 1 (UBA1); protein i... 152 4e-36
gb|AAL90910.1| At2g30110/T27E13.15 [Arabidopsis thaliana] 152 4e-36
ref|NP_568168.1| ubiquitin activating enzyme 2 (UBA2); protein i... 151 8e-36
sp|P20973|UBA1_WHEAT Ubiquitin-activating enzyme E1 1 gi|100841|... 135 7e-31
sp|P31252|UBA3_WHEAT Ubiquitin-activating enzyme E1 3 gi|7437981... 134 1e-30
>ref|NP_565693.1| ubiquitin activating enzyme 1 (UBA1); protein id: At2g30110.1,
supported by cDNA: gi_19699086 [Arabidopsis thaliana]
gi|7437978|pir||T00587 probable ubiquitin-protein ligase
(EC 6.3.2.19) - Arabidopsis thaliana
gi|1750376|gb|AAB39246.1| ubiquitin activating enzyme
[Arabidopsis thaliana] gi|3150409|gb|AAC16961.1|
ubiquitin activating enzyme 1 (UBA1) [Arabidopsis
thaliana]
Length = 1080
Score = 152 bits (384), Expect = 4e-36
Identities = 80/124 (64%), Positives = 99/124 (79%)
Frame = +3
Query: 342 RVNRRSTRATGASATAAAGTATVYGSSGNTIGGASSEMALGDSHPMEIDEDLHSRQLAVY 521
R++ + + +S+ A+G++ G G+++ +MA G+S+ EIDEDLHSRQLAVY
Sbjct: 29 RIDFTESSSDKSSSILASGSSR--GFHGDSVV-QQIDMAFGNSNRQEIDEDLHSRQLAVY 85
Query: 522 GRETMRRLFASNVLVSGMRGLGVEIAKNLVLAGVKSVTLHDEGTVELWDLSSNFVFAEDD 701
GRETMRRLFASNVL+SGM GLG EIAKNL+LAGVKSVTLHDE VELWDLSSNFVF+EDD
Sbjct: 86 GRETMRRLFASNVLISGMHGLGAEIAKNLILAGVKSVTLHDERVVELWDLSSNFVFSEDD 145
Query: 702 LGKN 713
+GKN
Sbjct: 146 VGKN 149
Score = 41.6 bits (96), Expect = 0.011
Identities = 23/76 (30%), Positives = 41/76 (53%), Gaps = 5/76 (6%)
Frame = +3
Query: 498 HSRQLAVYGRETMRRLFASNVLVSGMRGLGVEIAKNLVLAGVK-----SVTLHDEGTVEL 662
+ Q++V+G + ++L + V G LG E KNL L GV +T+ D+ +E
Sbjct: 475 YDAQISVFGAKFQKKLEDAKVFTVGSGALGCEFLKNLALMGVSCGSQGKLTVTDDDIIEK 534
Query: 663 WDLSSNFVFAEDDLGK 710
+LS F+F + ++G+
Sbjct: 535 SNLSRQFLFRDWNIGQ 550
>gb|AAL90910.1| At2g30110/T27E13.15 [Arabidopsis thaliana]
Length = 1080
Score = 152 bits (384), Expect = 4e-36
Identities = 80/124 (64%), Positives = 99/124 (79%)
Frame = +3
Query: 342 RVNRRSTRATGASATAAAGTATVYGSSGNTIGGASSEMALGDSHPMEIDEDLHSRQLAVY 521
R++ + + +S+ A+G++ G G+++ +MA G+S+ EIDEDLHSRQLAVY
Sbjct: 29 RIDFTESSSDKSSSILASGSSR--GFHGDSVV-QQIDMAFGNSNRQEIDEDLHSRQLAVY 85
Query: 522 GRETMRRLFASNVLVSGMRGLGVEIAKNLVLAGVKSVTLHDEGTVELWDLSSNFVFAEDD 701
GRETMRRLFASNVL+SGM GLG EIAKNL+LAGVKSVTLHDE VELWDLSSNFVF+EDD
Sbjct: 86 GRETMRRLFASNVLISGMHGLGAEIAKNLILAGVKSVTLHDERVVELWDLSSNFVFSEDD 145
Query: 702 LGKN 713
+GKN
Sbjct: 146 VGKN 149
Score = 41.6 bits (96), Expect = 0.011
Identities = 23/76 (30%), Positives = 41/76 (53%), Gaps = 5/76 (6%)
Frame = +3
Query: 498 HSRQLAVYGRETMRRLFASNVLVSGMRGLGVEIAKNLVLAGVK-----SVTLHDEGTVEL 662
+ Q++V+G + ++L + V G LG E KNL L GV +T+ D+ +E
Sbjct: 475 YDAQISVFGAKFQKKLEDAKVFTVGSGALGCEFLKNLALMGVSCGSQGKLTVTDDDIIEK 534
Query: 663 WDLSSNFVFAEDDLGK 710
+LS F+F + ++G+
Sbjct: 535 SNLSRQFLFRDWNIGQ 550
>ref|NP_568168.1| ubiquitin activating enzyme 2 (UBA2); protein id: At5g06460.1
[Arabidopsis thaliana] gi|1703478|gb|AAB37569.1|
ubiquitin activating enzyme 2 gi|9758426|dbj|BAB08968.1|
ubiquitin activating enzyme 2 [Arabidopsis thaliana]
Length = 1077
Score = 151 bits (382), Expect = 8e-36
Identities = 78/123 (63%), Positives = 97/123 (78%), Gaps = 5/123 (4%)
Frame = +3
Query: 360 TRATGASATAAAGTATVYGSSGNTIGGASSEMALGD-----SHPMEIDEDLHSRQLAVYG 524
T + SA A+ ++++ N+IGG + M++ + S+ EIDEDLHSRQLAVYG
Sbjct: 26 TESADGSAINASNSSSI--GLNNSIGGNDTVMSMAEFGNDNSNNQEIDEDLHSRQLAVYG 83
Query: 525 RETMRRLFASNVLVSGMRGLGVEIAKNLVLAGVKSVTLHDEGTVELWDLSSNFVFAEDDL 704
RETMR+LFASNVL+SGM+GLGVEIAKN++LAGVKSVTLHDE VELWDLSSNFVF E+D+
Sbjct: 84 RETMRKLFASNVLISGMQGLGVEIAKNIILAGVKSVTLHDENVVELWDLSSNFVFTEEDI 143
Query: 705 GKN 713
GKN
Sbjct: 144 GKN 146
Score = 43.1 bits (100), Expect = 0.004
Identities = 24/76 (31%), Positives = 41/76 (53%), Gaps = 5/76 (6%)
Frame = +3
Query: 498 HSRQLAVYGRETMRRLFASNVLVSGMRGLGVEIAKNLVLAGVK-----SVTLHDEGTVEL 662
+ Q++V+G ++L + V V G LG E KNL L GV +T+ D+ +E
Sbjct: 472 YDAQISVFGSTLQKKLEDARVFVVGAGALGCEFLKNLALMGVSCGTQGKLTVTDDDVIEK 531
Query: 663 WDLSSNFVFAEDDLGK 710
+LS F+F + ++G+
Sbjct: 532 SNLSRQFLFRDWNIGQ 547
>sp|P20973|UBA1_WHEAT Ubiquitin-activating enzyme E1 1 gi|100841|pir||A38373
ubiquitin-protein ligase (EC 6.3.2.19) E1 - wheat
gi|170780|gb|AAA34308.1| ubiquitin-activating enzyme E1
Length = 1051
Score = 135 bits (339), Expect = 7e-31
Identities = 65/78 (83%), Positives = 73/78 (93%)
Frame = +3
Query: 480 EIDEDLHSRQLAVYGRETMRRLFASNVLVSGMRGLGVEIAKNLVLAGVKSVTLHDEGTVE 659
EIDEDLHSRQLAVYGRETM+RLF SNVLVSG++GLG EIAKNLVLAGVKSVTLHD+G VE
Sbjct: 42 EIDEDLHSRQLAVYGRETMKRLFGSNVLVSGLQGLGAEIAKNLVLAGVKSVTLHDDGNVE 101
Query: 660 LWDLSSNFVFAEDDLGKN 713
LWDLSSNF +E+D+G+N
Sbjct: 102 LWDLSSNFFLSENDVGQN 119
Score = 43.1 bits (100), Expect = 0.004
Identities = 22/76 (28%), Positives = 42/76 (54%), Gaps = 5/76 (6%)
Frame = +3
Query: 498 HSRQLAVYGRETMRRLFASNVLVSGMRGLGVEIAKNLVLAGVK-----SVTLHDEGTVEL 662
+ Q++V+G + +L + + + G LG E KNL L G+ ++TL D+ +E
Sbjct: 445 YDAQISVFGSKLQNKLEEAKIFMVGSGALGCEFLKNLALMGISCSQNGNLTLTDDDVIEK 504
Query: 663 WDLSSNFVFAEDDLGK 710
+LS F+F + ++G+
Sbjct: 505 SNLSRQFLFRDWNIGQ 520
>sp|P31252|UBA3_WHEAT Ubiquitin-activating enzyme E1 3 gi|7437981|pir||T06483 probable
ubiquitin-protein ligase (EC 6.3.2.19) E1 - wheat
gi|170686|gb|AAA34266.1| ubiquitin activating enzyme
Length = 1053
Score = 134 bits (338), Expect = 1e-30
Identities = 71/122 (58%), Positives = 83/122 (67%)
Frame = +3
Query: 348 NRRSTRATGASATAAAGTATVYGSSGNTIGGASSEMALGDSHPMEIDEDLHSRQLAVYGR 527
++R + A G A GS A+ + P EIDEDLHSRQLAVYGR
Sbjct: 4 SKRPSDAAAGDENGRGGDARGPGSGRRRARAAAGAVTAA---PQEIDEDLHSRQLAVYGR 60
Query: 528 ETMRRLFASNVLVSGMRGLGVEIAKNLVLAGVKSVTLHDEGTVELWDLSSNFVFAEDDLG 707
ETMRRLFAS+VLVSG+ GLG EIAKNL LAGVKSVT+HD TV++WDLS NF +EDD+G
Sbjct: 61 ETMRRLFASDVLVSGLNGLGAEIAKNLALAGVKSVTIHDVKTVKMWDLSGNFFLSEDDIG 120
Query: 708 KN 713
KN
Sbjct: 121 KN 122
Score = 43.9 bits (102), Expect = 0.002
Identities = 23/76 (30%), Positives = 42/76 (55%), Gaps = 5/76 (6%)
Frame = +3
Query: 498 HSRQLAVYGRETMRRLFASNVLVSGMRGLGVEIAKNLVLAGVK-----SVTLHDEGTVEL 662
+ Q++V+G + +++ +N V G LG E KNL L GV +T+ D+ +E
Sbjct: 448 YDAQVSVFGSKLQKKMEEANTFVVGSGALGCEFLKNLALMGVSCSSKGKLTITDDDIIEK 507
Query: 663 WDLSSNFVFAEDDLGK 710
+LS F+F + ++G+
Sbjct: 508 SNLSRQFLFRDWNIGQ 523
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 674,930,447
Number of Sequences: 1393205
Number of extensions: 16914470
Number of successful extensions: 176022
Number of sequences better than 10.0: 537
Number of HSP's better than 10.0 without gapping: 89712
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 149957
length of database: 448,689,247
effective HSP length: 120
effective length of database: 281,504,647
effective search space used: 32936043699
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
