Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC017230A_C01 KMC017230A_c01
(512 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_199961.1| pectinesterase (pectin methylesterase) family; ... 77 1e-13
ref|NP_194254.2| pectinesterase (pectin methylesterase) family; ... 68 5e-11
ref|NP_192979.1| pectinesterase (pectin methylesterase) family; ... 68 7e-11
pir||T05545 pollen-specific protein homolog F24A6.80 - Arabidops... 56 3e-07
dbj|BAA90610.1| ESTs AU082304(C61278),C93463(C50982) correspond ... 49 4e-05
>ref|NP_199961.1| pectinesterase (pectin methylesterase) family; protein id:
At5g51480.1 [Arabidopsis thaliana]
gi|9758190|dbj|BAB08664.1| pectinesterase-like; strong
similarity to pollen-specific protein [Arabidopsis
thaliana] gi|28393459|gb|AAO42151.1| unknown protein
[Arabidopsis thaliana] gi|28827358|gb|AAO50523.1|
unknown protein [Arabidopsis thaliana]
Length = 592
Score = 77.0 bits (188), Expect = 1e-13
Identities = 38/72 (52%), Positives = 51/72 (70%)
Frame = -2
Query: 511 GQETYLRIVNPEENGATEMAQPQNVLYCGPLGYLQKKQPHSSAASTFGYFSNLFTLILGL 332
GQETY+RI+NPEENG+TEM P+NV+YCG L +QK+Q HSSA T + LI +
Sbjct: 523 GQETYMRIINPEENGSTEMDPPENVMYCGALQAMQKEQHHSSA--TKSMTNGQLILIFSM 580
Query: 331 LIVVFTSS*SFC 296
++V+ +S SFC
Sbjct: 581 MMVLLSSFSSFC 592
>ref|NP_194254.2| pectinesterase (pectin methylesterase) family; protein id:
At4g25240.1, supported by cDNA: gi_18389263, supported
by cDNA: gi_20259624 [Arabidopsis thaliana]
gi|18389264|gb|AAL67075.1| putative Pollen-specific
protein precursor [Arabidopsis thaliana]
gi|20259625|gb|AAM14169.1| putative pollen-specific
protein precursor [Arabidopsis thaliana]
Length = 589
Score = 68.2 bits (165), Expect = 5e-11
Identities = 38/72 (52%), Positives = 45/72 (61%)
Frame = -2
Query: 511 GQETYLRIVNPEENGATEMAQPQNVLYCGPLGYLQKKQPHSSAASTFGYFSNLFTLILGL 332
G+ETY+RI NPEE+G TEM P NVLYCG L LQK+Q HS+A S L L L
Sbjct: 523 GEETYMRITNPEEDGKTEMDPPDNVLYCGALKNLQKEQHHSAATSILNGH-----LKLML 577
Query: 331 LIVVFTSS*SFC 296
L+V+ S FC
Sbjct: 578 LMVLLASVFRFC 589
>ref|NP_192979.1| pectinesterase (pectin methylesterase) family; protein id:
At4g12420.1, supported by cDNA: gi_18158153 [Arabidopsis
thaliana] gi|7433001|pir||T07634 pollen-specific protein
homolog T1P17.10 - Arabidopsis thaliana
gi|4725941|emb|CAB41712.1| putative pollen-specific
protein [Arabidopsis thaliana]
gi|7267944|emb|CAB78285.1| putative pollen-specific
protein [Arabidopsis thaliana]
gi|18158154|gb|AAL62306.1|AF439406_1 multi-copper
oxidase-related protein [Arabidopsis thaliana]
Length = 587
Score = 67.8 bits (164), Expect = 7e-11
Identities = 32/64 (50%), Positives = 45/64 (70%)
Frame = -2
Query: 511 GQETYLRIVNPEENGATEMAQPQNVLYCGPLGYLQKKQPHSSAASTFGYFSNLFTLILGL 332
GQETY+R+VNP+EN TE P NVLYCG L LQK Q SS+AS F++L +++ L
Sbjct: 520 GQETYVRVVNPDENNKTEFGHPDNVLYCGALSKLQKPQKVSSSASKSIGFTSLSMVVMAL 579
Query: 331 LIVV 320
++++
Sbjct: 580 VMMM 583
>pir||T05545 pollen-specific protein homolog F24A6.80 - Arabidopsis thaliana
gi|4454012|emb|CAA23065.1| Pollen-specific protein
precursor like [Arabidopsis thaliana]
gi|7269375|emb|CAB81335.1| Pollen-specific protein
precursor like [Arabidopsis thaliana]
Length = 561
Score = 55.8 bits (133), Expect = 3e-07
Identities = 25/36 (69%), Positives = 28/36 (77%)
Frame = -2
Query: 511 GQETYLRIVNPEENGATEMAQPQNVLYCGPLGYLQK 404
G+ETY+RI NPEE+G TEM P NVLYCG L LQK
Sbjct: 523 GEETYMRITNPEEDGKTEMDPPDNVLYCGALKNLQK 558
>dbj|BAA90610.1| ESTs AU082304(C61278),C93463(C50982) correspond to a region of the
predicted gene.~Similar to Arabidopsis thaliana DNA
chromosome 4, BAC clone F24A6, Pollen-specific protein
precursor like. (AL035396) [Oryza sativa (japonica
cultivar-group)]
Length = 593
Score = 48.5 bits (114), Expect = 4e-05
Identities = 25/48 (52%), Positives = 33/48 (68%), Gaps = 2/48 (4%)
Frame = -2
Query: 511 GQETYLRIVNPEEN-GATEMAQPQNVLYCGPLGYLQKKQ-PHSSAAST 374
GQETY+R+V+P+ TEM P N+LYCG L QK Q PH S++S+
Sbjct: 522 GQETYIRVVDPDGGYNVTEMVAPDNMLYCGLLKDKQKAQKPHGSSSSS 569
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 417,322,106
Number of Sequences: 1393205
Number of extensions: 8051340
Number of successful extensions: 16349
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 16030
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 16340
length of database: 448,689,247
effective HSP length: 114
effective length of database: 289,863,877
effective search space used: 16232377112
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)