Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC016521A_C01 KMC016521A_c01
(564 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||T11688 phosphoinositide-specific phospholipase C (EC 3.1.4.... 148 5e-35
pir||T06771 phosphoinositide-specific phospholipase C (EC 3.1.4.... 137 1e-31
gb|AAN75042.1| phosphoinositide-specific phospholipase C 8 [Arab... 125 4e-28
ref|NP_671947.1| similar to phosphoinositide specific phospholip... 125 4e-28
pir||T07421 phosphoinositide-specific phospholipase C (EC 3.1.4.... 120 1e-26
>pir||T11688 phosphoinositide-specific phospholipase C (EC 3.1.4.-) - cowpea
gi|1786115|gb|AAB41107.1| phosphoinositide-specific
phospholipase C [Vigna unguiculata]
Length = 549
Score = 148 bits (373), Expect = 5e-35
Identities = 69/79 (87%), Positives = 73/79 (92%)
Frame = -2
Query: 557 ISNNWFPVWNEEFDFPLTVPELALLRIDVREHDSHGKDELGGQTCLPVSELKSGFRSVPL 378
ISNNWFPVWNEEFDFPLTVPELALL I+VRE D H KD+ GGQTCLPVSELKSGFRSVPL
Sbjct: 470 ISNNWFPVWNEEFDFPLTVPELALLGIEVREDDKHQKDDFGGQTCLPVSELKSGFRSVPL 529
Query: 377 YDEKGDPFKSVKLLMRFRF 321
YDEKGD +KSVKLLMRF+F
Sbjct: 530 YDEKGDKYKSVKLLMRFQF 548
>pir||T06771 phosphoinositide-specific phospholipase C (EC 3.1.4.-) P12 -
soybean gi|1399303|gb|AAB03257.1|
phosphoinositide-specific phospholipase C P12
Length = 553
Score = 137 bits (344), Expect = 1e-31
Identities = 60/79 (75%), Positives = 73/79 (91%)
Frame = -2
Query: 557 ISNNWFPVWNEEFDFPLTVPELALLRIDVREHDSHGKDELGGQTCLPVSELKSGFRSVPL 378
I +NWFPVW+EEF+FPLTVPELALLRI+VRE+D H KD+ GGQTCLP+SEL+SGFR+VPL
Sbjct: 474 IQDNWFPVWDEEFEFPLTVPELALLRIEVREYDKHEKDDFGGQTCLPISELRSGFRAVPL 533
Query: 377 YDEKGDPFKSVKLLMRFRF 321
+D+KG+ KSVKLLMRF+F
Sbjct: 534 FDQKGEQLKSVKLLMRFQF 552
>gb|AAN75042.1| phosphoinositide-specific phospholipase C 8 [Arabidopsis thaliana]
Length = 544
Score = 125 bits (314), Expect = 4e-28
Identities = 56/79 (70%), Positives = 68/79 (85%)
Frame = -2
Query: 557 ISNNWFPVWNEEFDFPLTVPELALLRIDVREHDSHGKDELGGQTCLPVSELKSGFRSVPL 378
I +NW+P+W+EEF FPLTVPELALLRI+VRE+D KD+ GGQTCLPV+EL+ G RSVPL
Sbjct: 465 IEDNWYPIWDEEFSFPLTVPELALLRIEVREYDMSEKDDFGGQTCLPVAELRPGIRSVPL 524
Query: 377 YDEKGDPFKSVKLLMRFRF 321
YD+KG+ KSV+LLMRF F
Sbjct: 525 YDKKGEKMKSVRLLMRFIF 543
>ref|NP_671947.1| similar to phosphoinositide specific phospholipase C(AtPLC2);
protein id: At2g40116.1 [Arabidopsis thaliana]
Length = 602
Score = 125 bits (314), Expect = 4e-28
Identities = 56/79 (70%), Positives = 68/79 (85%)
Frame = -2
Query: 557 ISNNWFPVWNEEFDFPLTVPELALLRIDVREHDSHGKDELGGQTCLPVSELKSGFRSVPL 378
I +NW+P+W+EEF FPLTVPELALLRI+VRE+D KD+ GGQTCLPV+EL+ G RSVPL
Sbjct: 523 IEDNWYPIWDEEFSFPLTVPELALLRIEVREYDMSEKDDFGGQTCLPVAELRPGIRSVPL 582
Query: 377 YDEKGDPFKSVKLLMRFRF 321
YD+KG+ KSV+LLMRF F
Sbjct: 583 YDKKGEKMKSVRLLMRFIF 601
>pir||T07421 phosphoinositide-specific phospholipase C (EC 3.1.4.-) PLC1 -
potato gi|2853041|emb|CAA63777.1|
1-phosphatidylinositol-4,5-bisphosphate
phosphodiesterase; phosphoinositide-specific
phospholipase C [Solanum tuberosum]
Length = 596
Score = 120 bits (301), Expect = 1e-26
Identities = 51/79 (64%), Positives = 67/79 (84%)
Frame = -2
Query: 557 ISNNWFPVWNEEFDFPLTVPELALLRIDVREHDSHGKDELGGQTCLPVSELKSGFRSVPL 378
I +NW P+WN+EF+FPLTVPELALLR++V E+D D+ GGQTC+PVSEL++G R+VP+
Sbjct: 516 IEDNWIPIWNDEFEFPLTVPELALLRVEVHEYDMSEIDDFGGQTCIPVSELRTGIRAVPI 575
Query: 377 YDEKGDPFKSVKLLMRFRF 321
Y+EKG+ + SVKLLMRF F
Sbjct: 576 YNEKGEKYPSVKLLMRFEF 594
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 450,393,029
Number of Sequences: 1393205
Number of extensions: 8935381
Number of successful extensions: 20590
Number of sequences better than 10.0: 257
Number of HSP's better than 10.0 without gapping: 20054
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 20554
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 20382500157
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
