Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC016397A_C01 KMC016397A_c01
(657 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_180566.1| G-protein beta family; protein id: At2g30050.1,... 241 5e-63
dbj|BAB83081.1| Sec13p [Oryza sativa] 241 8e-63
gb|AAM65095.1| putative protein transport protein SEC13 [Arabido... 236 2e-61
ref|NP_186783.1| transport protein SEC13, putative; protein id: ... 236 3e-61
gb|AAM65185.1| transport protein SEC13, putative [Arabidopsis th... 227 1e-58
>ref|NP_180566.1| G-protein beta family; protein id: At2g30050.1, supported by cDNA:
36577., supported by cDNA: gi_15724343 [Arabidopsis
thaliana] gi|7446122|pir||T02480 sec13-related protein
At2g30050 - Arabidopsis thaliana
gi|3150415|gb|AAC16967.1| putative protein transport
protein SEC13 [Arabidopsis thaliana]
gi|15724344|gb|AAL06565.1|AF412112_1 At2g30050/F23F1.3
[Arabidopsis thaliana] gi|20197232|gb|AAM14986.1|
putative protein transport protein SEC13 [Arabidopsis
thaliana] gi|21700867|gb|AAM70557.1| At2g30050/F23F1.3
[Arabidopsis thaliana]
Length = 302
Score = 241 bits (616), Expect = 5e-63
Identities = 108/127 (85%), Positives = 116/127 (91%)
Frame = -1
Query: 657 VQKLCSGGCDNTVKVWKLNHGLWKMDCFPALQMHTDWVRDVAWAPNLGLPKSTIASASQD 478
V KL SGGCDNTVKVWKL +G WKMDCFPALQ HTDWVRDVAWAPNLGLPKSTIAS SQD
Sbjct: 176 VYKLASGGCDNTVKVWKLANGSWKMDCFPALQKHTDWVRDVAWAPNLGLPKSTIASGSQD 235
Query: 477 GKVIIWTVAKEGDQWEGRVLNDFKTPVWRASWSLTGNILAVADGNNNVTLWKEAVDGDWQ 298
GKVIIWTV KEG+QWEG+VL DF TPVWR SWSLTGN+LAV+DGNNNVT+WKEAVDG+W+
Sbjct: 236 GKVIIWTVGKEGEQWEGKVLKDFMTPVWRVSWSLTGNLLAVSDGNNNVTVWKEAVDGEWE 295
Query: 297 QVTTVEP 277
QVT VEP
Sbjct: 296 QVTAVEP 302
Score = 47.8 bits (112), Expect = 1e-04
Identities = 33/121 (27%), Positives = 56/121 (46%), Gaps = 3/121 (2%)
Frame = -1
Query: 654 QKLCSGGCDNTVKVWKLNHGLWKMDCFPALQMHTDWVRDVAWA-PNLGLPKSTIASASQD 478
+++ + D T+K+ +++ L H V +VAWA P G S +AS S D
Sbjct: 24 KRIATASSDCTIKITGVSNNGGSQQ-LATLTGHRGPVWEVAWAHPKYG---SILASCSYD 79
Query: 477 GKVIIWTVAKEGDQWEGRVLNDFKTPVWRASWSL--TGNILAVADGNNNVTLWKEAVDGD 304
G+VI+W + + V D K+ V +W+ G LA + N++++ DG
Sbjct: 80 GQVILWKEGNQNQWTQDHVFTDHKSSVNSIAWAPHDIGLSLACGSSDGNISVFTARADGG 139
Query: 303 W 301
W
Sbjct: 140 W 140
Score = 43.9 bits (102), Expect = 0.002
Identities = 29/90 (32%), Positives = 39/90 (43%), Gaps = 2/90 (2%)
Frame = -1
Query: 558 HTDWVRDVAWAPNLGLPKSTIASASQDGKVIIWTVAKEGDQWEGRVLNDFKTPVWRASWS 379
H D V DV + IA+AS D + I V+ G + L + PVW +W+
Sbjct: 10 HEDIVHDV----QMDYYGKRIATASSDCTIKITGVSNNGGSQQLATLTGHRGPVWEVAWA 65
Query: 378 LT--GNILAVADGNNNVTLWKEAVDGDWQQ 295
G+ILA + V LWKE W Q
Sbjct: 66 HPKYGSILASCSYDGQVILWKEGNQNQWTQ 95
Score = 40.0 bits (92), Expect = 0.027
Identities = 37/133 (27%), Positives = 55/133 (40%), Gaps = 16/133 (12%)
Frame = -1
Query: 648 LCSGGCDNTVKVWKL-NHGLWKMDCFPALQMHTDWVRDVAWAPN-LGLPKSTIASASQDG 475
L S D V +WK N W D H V +AWAP+ +GL ++A S DG
Sbjct: 73 LASCSYDGQVILWKEGNQNQWTQD--HVFTDHKSSVNSIAWAPHDIGL---SLACGSSDG 127
Query: 474 KVIIWTVAKEGDQWEGRVLNDFKTPVWRASWS------------LTGNILAVADG--NNN 337
+ ++T +G R+ V SW+ L + +A G +N
Sbjct: 128 NISVFTARADGGWDTSRIDQAHPVGVTSVSWAPATAPGALVSSGLLDPVYKLASGGCDNT 187
Query: 336 VTLWKEAVDGDWQ 298
V +WK A +G W+
Sbjct: 188 VKVWKLA-NGSWK 199
>dbj|BAB83081.1| Sec13p [Oryza sativa]
Length = 305
Score = 241 bits (614), Expect = 8e-63
Identities = 109/126 (86%), Positives = 117/126 (92%)
Frame = -1
Query: 657 VQKLCSGGCDNTVKVWKLNHGLWKMDCFPALQMHTDWVRDVAWAPNLGLPKSTIASASQD 478
VQKL SGGCDNTVKVWKL +G W+MDCFPALQMH DWVRDVAWAPNLGLPKSTIASASQD
Sbjct: 179 VQKLASGGCDNTVKVWKLYNGSWRMDCFPALQMHRDWVRDVAWAPNLGLPKSTIASASQD 238
Query: 477 GKVIIWTVAKEGDQWEGRVLNDFKTPVWRASWSLTGNILAVADGNNNVTLWKEAVDGDWQ 298
G V+IWT KEG+QWEGRVL DF+TPVWR SWSLTGNILAV+DGN+NVTLWKEAVDG+WQ
Sbjct: 239 GTVVIWTAPKEGEQWEGRVLYDFQTPVWRLSWSLTGNILAVSDGNDNVTLWKEAVDGEWQ 298
Query: 297 QVTTVE 280
QVTTVE
Sbjct: 299 QVTTVE 304
Score = 50.1 bits (118), Expect = 3e-05
Identities = 35/122 (28%), Positives = 58/122 (46%), Gaps = 4/122 (3%)
Frame = -1
Query: 654 QKLCSGGCDNTVKVWKLNHGLWKMDCFPALQMHTDWVRDVAWA-PNLGLPKSTIASASQD 478
+++ + DNT+K+ ++ + L H V VAWA P G S +AS S D
Sbjct: 24 KRIATASSDNTIKIIGVSGNSHQQ--LATLSGHQGPVWQVAWAHPKYG---SLLASCSYD 78
Query: 477 GKVIIWTVAKEGDQW-EGRVLNDFKTPVWRASWS--LTGNILAVADGNNNVTLWKEAVDG 307
G+VIIW + D+W + + K+ V +W+ G LA + N++++ DG
Sbjct: 79 GRVIIWKEGSKPDEWAQAHTFIEHKSSVNSIAWAPHELGLCLACGSSDGNISVFTARSDG 138
Query: 306 DW 301
W
Sbjct: 139 GW 140
>gb|AAM65095.1| putative protein transport protein SEC13 [Arabidopsis thaliana]
Length = 302
Score = 236 bits (602), Expect = 2e-61
Identities = 107/127 (84%), Positives = 115/127 (90%)
Frame = -1
Query: 657 VQKLCSGGCDNTVKVWKLNHGLWKMDCFPALQMHTDWVRDVAWAPNLGLPKSTIASASQD 478
V KL SGGCDNTVKVWKL +G WKMDCFPALQ HTDWVRDVAWAPNLGLPKSTIAS SQD
Sbjct: 176 VYKLASGGCDNTVKVWKLANGSWKMDCFPALQKHTDWVRDVAWAPNLGLPKSTIASGSQD 235
Query: 477 GKVIIWTVAKEGDQWEGRVLNDFKTPVWRASWSLTGNILAVADGNNNVTLWKEAVDGDWQ 298
GKVIIWTV KEG+QWEG+VL DF TPVWR S SLTGN+LAV+DGNNNVT+WKEAVDG+W+
Sbjct: 236 GKVIIWTVGKEGEQWEGKVLKDFMTPVWRVSSSLTGNLLAVSDGNNNVTVWKEAVDGEWE 295
Query: 297 QVTTVEP 277
QVT VEP
Sbjct: 296 QVTAVEP 302
Score = 47.8 bits (112), Expect = 1e-04
Identities = 33/121 (27%), Positives = 56/121 (46%), Gaps = 3/121 (2%)
Frame = -1
Query: 654 QKLCSGGCDNTVKVWKLNHGLWKMDCFPALQMHTDWVRDVAWA-PNLGLPKSTIASASQD 478
+++ + D T+K+ +++ L H V +VAWA P G S +AS S D
Sbjct: 24 KRIATASSDCTIKITGVSNNGGSQQ-LATLTGHRGPVWEVAWAHPKYG---SILASCSYD 79
Query: 477 GKVIIWTVAKEGDQWEGRVLNDFKTPVWRASWSL--TGNILAVADGNNNVTLWKEAVDGD 304
G+VI+W + + V D K+ V +W+ G LA + N++++ DG
Sbjct: 80 GQVILWKEGNQNQWTQDHVFTDHKSSVNSIAWAPHDIGLSLACGSSDGNISVFTARADGG 139
Query: 303 W 301
W
Sbjct: 140 W 140
Score = 43.9 bits (102), Expect = 0.002
Identities = 29/90 (32%), Positives = 39/90 (43%), Gaps = 2/90 (2%)
Frame = -1
Query: 558 HTDWVRDVAWAPNLGLPKSTIASASQDGKVIIWTVAKEGDQWEGRVLNDFKTPVWRASWS 379
H D V DV + IA+AS D + I V+ G + L + PVW +W+
Sbjct: 10 HEDIVHDV----QMDYYGKRIATASSDCTIKITGVSNNGGSQQLATLTGHRGPVWEVAWA 65
Query: 378 LT--GNILAVADGNNNVTLWKEAVDGDWQQ 295
G+ILA + V LWKE W Q
Sbjct: 66 HPKYGSILASCSYDGQVILWKEGNQNQWTQ 95
Score = 40.0 bits (92), Expect = 0.027
Identities = 37/133 (27%), Positives = 55/133 (40%), Gaps = 16/133 (12%)
Frame = -1
Query: 648 LCSGGCDNTVKVWKL-NHGLWKMDCFPALQMHTDWVRDVAWAPN-LGLPKSTIASASQDG 475
L S D V +WK N W D H V +AWAP+ +GL ++A S DG
Sbjct: 73 LASCSYDGQVILWKEGNQNQWTQD--HVFTDHKSSVNSIAWAPHDIGL---SLACGSSDG 127
Query: 474 KVIIWTVAKEGDQWEGRVLNDFKTPVWRASWS------------LTGNILAVADG--NNN 337
+ ++T +G R+ V SW+ L + +A G +N
Sbjct: 128 NISVFTARADGGWDTSRIDQAHPVGVTSVSWAPATAPGALVSSGLLDPVYKLASGGCDNT 187
Query: 336 VTLWKEAVDGDWQ 298
V +WK A +G W+
Sbjct: 188 VKVWKLA-NGSWK 199
>ref|NP_186783.1| transport protein SEC13, putative; protein id: At3g01340.1,
supported by cDNA: 37331., supported by cDNA:
gi_13877998, supported by cDNA: gi_17104728 [Arabidopsis
thaliana] gi|6094550|gb|AAF03492.1|AC010676_2 putative
protein transport protein SEC13 [Arabidopsis thaliana]
gi|13877999|gb|AAK44077.1|AF370262_1 putative transport
protein SEC13 [Arabidopsis thaliana]
gi|17104729|gb|AAL34253.1| putative transport protein
SEC13 [Arabidopsis thaliana]
Length = 302
Score = 236 bits (601), Expect = 3e-61
Identities = 103/127 (81%), Positives = 115/127 (90%)
Frame = -1
Query: 657 VQKLCSGGCDNTVKVWKLNHGLWKMDCFPALQMHTDWVRDVAWAPNLGLPKSTIASASQD 478
V KL SGGCD+TVKVWK ++G WKMDCFPAL HTDWVRDVAWAPNLGLPKSTIAS S+D
Sbjct: 176 VYKLASGGCDSTVKVWKFSNGSWKMDCFPALNKHTDWVRDVAWAPNLGLPKSTIASGSED 235
Query: 477 GKVIIWTVAKEGDQWEGRVLNDFKTPVWRASWSLTGNILAVADGNNNVTLWKEAVDGDWQ 298
GKVIIWT+ KEG+QWEG VL DFKTPVWR SWSLTGN+LAV+DGNNNVT+WKE+VDG+W+
Sbjct: 236 GKVIIWTIGKEGEQWEGTVLKDFKTPVWRVSWSLTGNLLAVSDGNNNVTVWKESVDGEWE 295
Query: 297 QVTTVEP 277
QVT VEP
Sbjct: 296 QVTVVEP 302
Score = 45.8 bits (107), Expect = 5e-04
Identities = 32/121 (26%), Positives = 54/121 (44%), Gaps = 3/121 (2%)
Frame = -1
Query: 654 QKLCSGGCDNTVKVWKLNHGLWKMDCFPALQMHTDWVRDVAWA-PNLGLPKSTIASASQD 478
+++ + D T+K+ +++ L H V VAWA P G S +AS S D
Sbjct: 24 KRVATASSDCTIKITGVSNSGGSQH-LATLTGHRGPVWQVAWAHPKFG---SLLASCSYD 79
Query: 477 GKVIIWTVAKEGDQWEGRVLNDFKTPVWRASWS--LTGNILAVADGNNNVTLWKEAVDGD 304
G++I+W + + V D K V +W+ G LA + N++++ DG
Sbjct: 80 GQIILWKEGNQNQWTQAHVFTDHKVSVNSIAWAPHELGLSLACGASDGNISVFSARADGG 139
Query: 303 W 301
W
Sbjct: 140 W 140
Score = 42.7 bits (99), Expect = 0.004
Identities = 25/90 (27%), Positives = 40/90 (43%), Gaps = 2/90 (2%)
Frame = -1
Query: 558 HTDWVRDVAWAPNLGLPKSTIASASQDGKVIIWTVAKEGDQWEGRVLNDFKTPVWRASWS 379
H+D + DV + +A+AS D + I V+ G L + PVW+ +W+
Sbjct: 10 HSDTIHDVV----MDYYGKRVATASSDCTIKITGVSNSGGSQHLATLTGHRGPVWQVAWA 65
Query: 378 LT--GNILAVADGNNNVTLWKEAVDGDWQQ 295
G++LA + + LWKE W Q
Sbjct: 66 HPKFGSLLASCSYDGQIILWKEGNQNQWTQ 95
>gb|AAM65185.1| transport protein SEC13, putative [Arabidopsis thaliana]
Length = 301
Score = 227 bits (578), Expect = 1e-58
Identities = 101/127 (79%), Positives = 113/127 (88%)
Frame = -1
Query: 657 VQKLCSGGCDNTVKVWKLNHGLWKMDCFPALQMHTDWVRDVAWAPNLGLPKSTIASASQD 478
V KL SGGCD+TVKVWK ++G WKMDCFPAL HTDWVRDVAWAPNLGLPKSTIAS S+D
Sbjct: 176 VYKLASGGCDSTVKVWKFSNGSWKMDCFPALNKHTDWVRDVAWAPNLGLPKSTIASGSED 235
Query: 477 GKVIIWTVAKEGDQWEGRVLNDFKTPVWRASWSLTGNILAVADGNNNVTLWKEAVDGDWQ 298
GKVIIWT+ K G+QWEG VL DFKTPVWR SWSLT N+LAV+DGNNNVT+WKE+VDG+W+
Sbjct: 236 GKVIIWTIGK-GEQWEGTVLKDFKTPVWRVSWSLTANLLAVSDGNNNVTVWKESVDGEWE 294
Query: 297 QVTTVEP 277
QVT VEP
Sbjct: 295 QVTVVEP 301
Score = 45.8 bits (107), Expect = 5e-04
Identities = 32/121 (26%), Positives = 54/121 (44%), Gaps = 3/121 (2%)
Frame = -1
Query: 654 QKLCSGGCDNTVKVWKLNHGLWKMDCFPALQMHTDWVRDVAWA-PNLGLPKSTIASASQD 478
+++ + D T+K+ +++ L H V VAWA P G S +AS S D
Sbjct: 24 KRVATASSDCTIKITGVSNSGGSQH-LATLTGHRGPVWQVAWAHPKFG---SLLASCSYD 79
Query: 477 GKVIIWTVAKEGDQWEGRVLNDFKTPVWRASWS--LTGNILAVADGNNNVTLWKEAVDGD 304
G++I+W + + V D K V +W+ G LA + N++++ DG
Sbjct: 80 GQIILWKEGNQNQWTQAHVFTDHKVSVNSIAWAPHELGLSLACGASDGNISVFSARADGG 139
Query: 303 W 301
W
Sbjct: 140 W 140
Score = 42.7 bits (99), Expect = 0.004
Identities = 25/90 (27%), Positives = 40/90 (43%), Gaps = 2/90 (2%)
Frame = -1
Query: 558 HTDWVRDVAWAPNLGLPKSTIASASQDGKVIIWTVAKEGDQWEGRVLNDFKTPVWRASWS 379
H+D + DV + +A+AS D + I V+ G L + PVW+ +W+
Sbjct: 10 HSDTIHDVV----MDYYGKRVATASSDCTIKITGVSNSGGSQHLATLTGHRGPVWQVAWA 65
Query: 378 LT--GNILAVADGNNNVTLWKEAVDGDWQQ 295
G++LA + + LWKE W Q
Sbjct: 66 HPKFGSLLASCSYDGQIILWKEGNQNQWTQ 95
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 613,740,439
Number of Sequences: 1393205
Number of extensions: 14235137
Number of successful extensions: 42849
Number of sequences better than 10.0: 854
Number of HSP's better than 10.0 without gapping: 38268
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 42077
length of database: 448,689,247
effective HSP length: 119
effective length of database: 282,897,852
effective search space used: 28006887348
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
