Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC014678A_C01 KMC014678A_c01
(506 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAK21920.1|AF338252_1 BiP-isoform D [Glycine max] 254 9e-77
pir||T46574 dnaK-type molecular chaperone BiP precursor [similar... 255 1e-76
emb|CAC14168.1| putative luminal binding protein [Corylus avellana] 253 3e-76
sp|Q03684|BIP4_TOBAC Luminal binding protein 4 precursor (BiP 4)... 252 3e-76
sp|Q03685|BIP5_TOBAC Luminal binding protein 5 precursor (BiP 5)... 252 4e-76
>gb|AAK21920.1|AF338252_1 BiP-isoform D [Glycine max]
Length = 495
Score = 254 bits (649), Expect(2) = 9e-77
Identities = 128/136 (94%), Positives = 131/136 (96%)
Frame = +1
Query: 79 AFNDGERLIGEAAKNQFAANPERTIFDVKRLIGRKFEDKEVQRDMKLVPYKIVNKDGKPY 258
AF D ERLIGEAAKNQ A NPERTIFDVKRLIGRKFEDKEVQ+DMKLVPYKIVNKDGKPY
Sbjct: 74 AFTDSERLIGEAAKNQAAVNPERTIFDVKRLIGRKFEDKEVQKDMKLVPYKIVNKDGKPY 133
Query: 259 IQVKIKDGETKVFSPEEVSAMILTKMKETAEAFLGKKINDAVVTVPAYFNDAQRQATKDA 438
IQVKIKDGETKVFSPEE+SAM+L KMKETAEAFLGKKINDAVVTVPAYFNDAQRQATKDA
Sbjct: 134 IQVKIKDGETKVFSPEEISAMVLIKMKETAEAFLGKKINDAVVTVPAYFNDAQRQATKDA 193
Query: 439 GVIAGLNVARIINEPT 486
GVIAGLNVARIINEPT
Sbjct: 194 GVIAGLNVARIINEPT 209
Score = 54.3 bits (129), Expect(2) = 9e-77
Identities = 24/27 (88%), Positives = 25/27 (91%)
Frame = +3
Query: 3 VGVYRNVHVEIIANDQGNRITPSWVRF 83
VGVY+N HVEIIANDQGNRITPSWV F
Sbjct: 49 VGVYKNGHVEIIANDQGNRITPSWVAF 75
>pir||T46574 dnaK-type molecular chaperone BiP precursor [similarity] - soybean
gi|2642238|gb|AAB86942.1| endoplasmic reticulum
HSC70-cognate binding protein precursor [Glycine max]
Length = 668
Score = 255 bits (652), Expect(2) = 1e-76
Identities = 130/136 (95%), Positives = 131/136 (95%)
Frame = +1
Query: 79 AFNDGERLIGEAAKNQFAANPERTIFDVKRLIGRKFEDKEVQRDMKLVPYKIVNKDGKPY 258
AF D ERLIGEAAKN A NPERTIFDVKRLIGRKFEDKEVQRDMKLVPYKIVNKDGKPY
Sbjct: 74 AFTDSERLIGEAAKNLAAVNPERTIFDVKRLIGRKFEDKEVQRDMKLVPYKIVNKDGKPY 133
Query: 259 IQVKIKDGETKVFSPEEVSAMILTKMKETAEAFLGKKINDAVVTVPAYFNDAQRQATKDA 438
IQVKIKDGETKVFSPEE+SAMILTKMKETAEAFLGKKINDAVVTVPAYFNDAQRQATKDA
Sbjct: 134 IQVKIKDGETKVFSPEEISAMILTKMKETAEAFLGKKINDAVVTVPAYFNDAQRQATKDA 193
Query: 439 GVIAGLNVARIINEPT 486
GVIAGLNVARIINEPT
Sbjct: 194 GVIAGLNVARIINEPT 209
Score = 52.4 bits (124), Expect(2) = 1e-76
Identities = 23/27 (85%), Positives = 25/27 (92%)
Frame = +3
Query: 3 VGVYRNVHVEIIANDQGNRITPSWVRF 83
VGVY+N HVEIIAN+QGNRITPSWV F
Sbjct: 49 VGVYKNGHVEIIANNQGNRITPSWVAF 75
>emb|CAC14168.1| putative luminal binding protein [Corylus avellana]
Length = 668
Score = 253 bits (645), Expect(2) = 3e-76
Identities = 127/135 (94%), Positives = 130/135 (96%)
Frame = +1
Query: 82 FNDGERLIGEAAKNQFAANPERTIFDVKRLIGRKFEDKEVQRDMKLVPYKIVNKDGKPYI 261
F DGERLIGEAAKNQ A NPERTIFDVKRLIGRKFEDKEVQ+DMKLVPYKIVNKDGKPYI
Sbjct: 76 FTDGERLIGEAAKNQAAVNPERTIFDVKRLIGRKFEDKEVQKDMKLVPYKIVNKDGKPYI 135
Query: 262 QVKIKDGETKVFSPEEVSAMILTKMKETAEAFLGKKINDAVVTVPAYFNDAQRQATKDAG 441
QVKIKDGETKVFSPEE+SAMIL KMKETAEAFLGKKI DAVVTVPAYFNDAQRQATKDAG
Sbjct: 136 QVKIKDGETKVFSPEEISAMILIKMKETAEAFLGKKIKDAVVTVPAYFNDAQRQATKDAG 195
Query: 442 VIAGLNVARIINEPT 486
+IAGLNVARIINEPT
Sbjct: 196 IIAGLNVARIINEPT 210
Score = 53.9 bits (128), Expect(2) = 3e-76
Identities = 24/27 (88%), Positives = 25/27 (91%)
Frame = +3
Query: 3 VGVYRNVHVEIIANDQGNRITPSWVRF 83
VGVY+N HVEIIANDQGNRITPSWV F
Sbjct: 50 VGVYKNGHVEIIANDQGNRITPSWVGF 76
>sp|Q03684|BIP4_TOBAC Luminal binding protein 4 precursor (BiP 4) (78 kDa
glucose-regulated protein homolog 4) (GRP 78-4)
gi|100339|pir||S21879 dnaK-type molecular chaperone blp4
precursor - common tobacco gi|19811|emb|CAA42659.1|
luminal binding protein (BiP) [Nicotiana tabacum]
Length = 667
Score = 252 bits (644), Expect(2) = 3e-76
Identities = 126/136 (92%), Positives = 131/136 (95%)
Frame = +1
Query: 79 AFNDGERLIGEAAKNQFAANPERTIFDVKRLIGRKFEDKEVQRDMKLVPYKIVNKDGKPY 258
AF DGERLIGEAAKN A NPERT+FDVKRLIGRKF+DKEVQRDMKLVPYKIVNKDGKPY
Sbjct: 76 AFTDGERLIGEAAKNLAAVNPERTVFDVKRLIGRKFDDKEVQRDMKLVPYKIVNKDGKPY 135
Query: 259 IQVKIKDGETKVFSPEEVSAMILTKMKETAEAFLGKKINDAVVTVPAYFNDAQRQATKDA 438
IQVKIKDGETK+FSPEE+SAMILTKMKETAEA+LGKKI DAVVTVPAYFNDAQRQATKDA
Sbjct: 136 IQVKIKDGETKIFSPEEISAMILTKMKETAEAYLGKKIKDAVVTVPAYFNDAQRQATKDA 195
Query: 439 GVIAGLNVARIINEPT 486
GVIAGLNVARIINEPT
Sbjct: 196 GVIAGLNVARIINEPT 211
Score = 54.3 bits (129), Expect(2) = 3e-76
Identities = 24/27 (88%), Positives = 25/27 (91%)
Frame = +3
Query: 3 VGVYRNVHVEIIANDQGNRITPSWVRF 83
VGVY+N HVEIIANDQGNRITPSWV F
Sbjct: 51 VGVYKNGHVEIIANDQGNRITPSWVAF 77
>sp|Q03685|BIP5_TOBAC Luminal binding protein 5 precursor (BiP 5) (78 kDa
glucose-regulated protein homolog 5) (GRP 78-5)
gi|100340|pir||S21880 dnaK-type molecular chaperone blp5
precursor - common tobacco gi|19813|emb|CAA42660.1|
luminal binding protein (BiP) [Nicotiana tabacum]
Length = 668
Score = 252 bits (643), Expect(2) = 4e-76
Identities = 127/136 (93%), Positives = 131/136 (95%)
Frame = +1
Query: 79 AFNDGERLIGEAAKNQFAANPERTIFDVKRLIGRKFEDKEVQRDMKLVPYKIVNKDGKPY 258
AF DGERLIGEAAKNQ A NPERTIFDVKRLIGRKF+DKEVQRD KLVPY+IVNKDGKPY
Sbjct: 75 AFTDGERLIGEAAKNQAAVNPERTIFDVKRLIGRKFDDKEVQRDKKLVPYEIVNKDGKPY 134
Query: 259 IQVKIKDGETKVFSPEEVSAMILTKMKETAEAFLGKKINDAVVTVPAYFNDAQRQATKDA 438
IQVKIKDGETKVFSPEE+SAMILTKMKETAEA+LGKKI DAVVTVPAYFNDAQRQATKDA
Sbjct: 135 IQVKIKDGETKVFSPEEISAMILTKMKETAEAYLGKKIKDAVVTVPAYFNDAQRQATKDA 194
Query: 439 GVIAGLNVARIINEPT 486
GVIAGLNVARIINEPT
Sbjct: 195 GVIAGLNVARIINEPT 210
Score = 54.3 bits (129), Expect(2) = 4e-76
Identities = 24/27 (88%), Positives = 25/27 (91%)
Frame = +3
Query: 3 VGVYRNVHVEIIANDQGNRITPSWVRF 83
VGVY+N HVEIIANDQGNRITPSWV F
Sbjct: 50 VGVYKNGHVEIIANDQGNRITPSWVAF 76
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 442,582,528
Number of Sequences: 1393205
Number of extensions: 9425460
Number of successful extensions: 29631
Number of sequences better than 10.0: 1450
Number of HSP's better than 10.0 without gapping: 26788
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 28486
length of database: 448,689,247
effective HSP length: 114
effective length of database: 289,863,877
effective search space used: 15652649358
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
