Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC014177A_C01 KMC014177A_c01
(485 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_177798.1| putative translation initiation factor IF-2; pr... 199 1e-50
pir||G96796 hypothetical protein F28O16.18 [imported] - Arabidop... 197 4e-50
pir||H96796 hypothetical protein F28O16.19 [imported] - Arabidop... 192 2e-48
gb|AAN32916.1| translation initiation factor [Pisum sativum] 148 3e-35
ref|NP_173540.1| translation initiation factor eIF-2, putative; ... 136 1e-31
>ref|NP_177798.1| putative translation initiation factor IF-2; protein id:
At1g76720.1 [Arabidopsis thaliana]
gi|25406462|pir||F96795 hypothetical protein F28O16.9
[imported] - Arabidopsis thaliana
gi|6143889|gb|AAF04435.1|AC010718_4 putative translation
initiation factor IF-2; 35582-30916 [Arabidopsis
thaliana]
Length = 1224
Score = 199 bits (506), Expect = 1e-50
Identities = 104/158 (65%), Positives = 129/158 (80%), Gaps = 2/158 (1%)
Frame = +3
Query: 3 GPRRL*RSAAVQKKKKKKEKEKEKKAAAAAAGSAPANETVEVKA--EVVESKKNESKTKA 176
G + +AA +KKKKKKEK+KEKKAAAAA S A E + ++ E ++ KK ++K KA
Sbjct: 321 GEKETVETAAAKKKKKKKEKDKEKKAAAAATSSVEAKEEKQEESVTEPLQPKKKDAKGKA 380
Query: 177 ADKKLPKHVREMQEALARRKEAEERKKREEEEKLRKEEEERRRQEELERQAEEARRRKKE 356
A+KK+PKHVREMQEALARR+EAEERKK+EEEEKLRKEEEERRRQEELE QAEEA+R++KE
Sbjct: 381 AEKKIPKHVREMQEALARRQEAEERKKKEEEEKLRKEEEERRRQEELEAQAEEAKRKRKE 440
Query: 357 REKEKLLKKKQEGKLLTGKQKEEQRRLEAMRRQILSTG 470
+EKEKLL+KK EGKLLT KQK E ++ EA + Q+L+ G
Sbjct: 441 KEKEKLLRKKLEGKLLTAKQKTEAQKREAFKNQLLAAG 478
Score = 35.4 bits (80), Expect = 0.34
Identities = 37/150 (24%), Positives = 68/150 (44%), Gaps = 21/150 (14%)
Frame = +3
Query: 60 KEKEKKAAAAAAGSAPANETVEVKA------EVVES----KKNESKTKAA-----DKKLP 194
K+K A+ + A+ET + K EV+E+ KK ++K K A + L
Sbjct: 219 KKKGSIVLASLGDDSVADETSQAKTPDTKSVEVIETGKIKKKKKNKNKVARTLEEEDDLD 278
Query: 195 KHVREMQEALARRKEAEERKKREEEEKLRKEE------EERRRQEELERQAEEARRRKKE 356
K + E+ E A + A E EK++ + E +E + E A +KK+
Sbjct: 279 KLLAELGETPAAERPASSTP---EVEKVQAQPGPVAPVENAGEKEGEKETVETAAAKKKK 335
Query: 357 REKEKLLKKKQEGKLLTGKQKEEQRRLEAM 446
++KEK +KK + + +E+++ E++
Sbjct: 336 KKKEKDKEKKAAAAATSSVEAKEEKQEESV 365
>pir||G96796 hypothetical protein F28O16.18 [imported] - Arabidopsis thaliana
gi|6143896|gb|AAF04442.1|AC010718_11 putative
translation initiation factor IF-2; 73082-68138
[Arabidopsis thaliana]
Length = 1280
Score = 197 bits (502), Expect = 4e-50
Identities = 108/157 (68%), Positives = 127/157 (80%), Gaps = 8/157 (5%)
Frame = +3
Query: 24 SAAVQKKKKKKEKEKEKKAAAAAAGSAPANETVEVKAEVVES--------KKNESKTKAA 179
+AA +KKKKKKEKEKEKKAAAAAA A +VEVK E E KK ++K KAA
Sbjct: 350 TAAAKKKKKKKEKEKEKKAAAAAA----ATSSVEVKEEKQEESVTEPLQPKKKDAKGKAA 405
Query: 180 DKKLPKHVREMQEALARRKEAEERKKREEEEKLRKEEEERRRQEELERQAEEARRRKKER 359
+KK+PKHVREMQEALARR+EAEERKK+EEEEKLRKEEEERRRQEELE QAEEA+R++KE+
Sbjct: 406 EKKIPKHVREMQEALARRQEAEERKKKEEEEKLRKEEEERRRQEELEAQAEEAKRKRKEK 465
Query: 360 EKEKLLKKKQEGKLLTGKQKEEQRRLEAMRRQILSTG 470
EKEKLL+KK EGKLLT KQK E ++ EA + Q+L+ G
Sbjct: 466 EKEKLLRKKLEGKLLTAKQKTEAQKREAFKNQLLAAG 502
Score = 42.0 bits (97), Expect = 0.004
Identities = 40/148 (27%), Positives = 69/148 (46%), Gaps = 14/148 (9%)
Frame = +3
Query: 45 KKKKEKEKEKKAAAAAAGSAPANETVEV---KAEVVESKKNESKTKAADKKLPKHVREMQ 215
KKK K+K A+ + A+ET EVVE+ K++ K K + K + V+E +
Sbjct: 244 KKKSSKKKGGSVLASVGDDSVADETKTSDTKNVEVVETGKSKKKKK--NNKSGRTVQEEE 301
Query: 216 E------ALARRKEAEE-RKKREEEEKLRKEEE----ERRRQEELERQAEEARRRKKERE 362
+ AL AE EEK + E E ++E E + A+++KK++E
Sbjct: 302 DLDKLLAALGETPAAERPASSTPVEEKAAQPEPVAPVENAGEKEGEEETAAAKKKKKKKE 361
Query: 363 KEKLLKKKQEGKLLTGKQKEEQRRLEAM 446
KEK K + + +E+++ E++
Sbjct: 362 KEKEKKAAAAAAATSSVEVKEEKQEESV 389
>pir||H96796 hypothetical protein F28O16.19 [imported] - Arabidopsis thaliana
gi|6143897|gb|AAF04443.1|AC010718_12 putative
translation initiation factor IF-2; 74568-78972
[Arabidopsis thaliana]
Length = 1146
Score = 192 bits (488), Expect = 2e-48
Identities = 100/153 (65%), Positives = 126/153 (81%), Gaps = 4/153 (2%)
Frame = +3
Query: 24 SAAVQKKKKKKEKEKEKKAAAAAAGSAPANETVEVKAEVV----ESKKNESKTKAADKKL 191
+AA +KKKKKKEKEKEKKAAAAAA ++ E + E V + +K ++K KAA+KK+
Sbjct: 236 TAAAKKKKKKKEKEKEKKAAAAAAATSSVEAKEEKQEESVTEPLQPRKKDAKGKAAEKKI 295
Query: 192 PKHVREMQEALARRKEAEERKKREEEEKLRKEEEERRRQEELERQAEEARRRKKEREKEK 371
PKHVRE+QEALARR+EA+ERKK+EEEEKLRKEEEERRRQEEL+ QAEEA+R++KE+EKEK
Sbjct: 296 PKHVREIQEALARRQEAKERKKKEEEEKLRKEEEERRRQEELDAQAEEAKRKRKEKEKEK 355
Query: 372 LLKKKQEGKLLTGKQKEEQRRLEAMRRQILSTG 470
LL+KK EGKLLT KQK E ++ EA + Q+L+ G
Sbjct: 356 LLRKKLEGKLLTAKQKSEAQKREAFKNQLLAAG 388
Score = 49.3 bits (116), Expect = 2e-05
Identities = 36/119 (30%), Positives = 58/119 (48%), Gaps = 8/119 (6%)
Frame = +3
Query: 39 KKKKKKEKEKEKKAAAAAAGSAPANETVEVKAEVVES-------KKNES-KTKAADKKLP 194
+KK K+K A+ S+ + + E+VES KKN+S +T D L
Sbjct: 134 RKKSSKKKSSSVLASIGDEESSQSKTSDSKSVELVESARSKKHKKKNKSGRTLQEDDDLD 193
Query: 195 KHVREMQEALARRKEAEERKKREEEEKLRKEEEERRRQEELERQAEEARRRKKEREKEK 371
K + E+ E A K A E +K + + + E +E +E A + +++KKE+EKEK
Sbjct: 194 KLLAELGETPAAGKPASEEEKDQAQPEPVAPVENTGEEETVETAAAKKKKKKKEKEKEK 252
>gb|AAN32916.1| translation initiation factor [Pisum sativum]
Length = 861
Score = 148 bits (374), Expect = 3e-35
Identities = 80/93 (86%), Positives = 85/93 (91%), Gaps = 1/93 (1%)
Frame = +3
Query: 210 MQEALARRKEAEERKKREEEEKLRKEEEERRRQEELERQAEEARRRKKEREKEKLLKKKQ 389
MQE LARRKEAEE++KREEEEKLRKEEEER+R EELERQAEEA+RRKKE+EKEKL KKK
Sbjct: 1 MQELLARRKEAEEKQKREEEEKLRKEEEERQRLEELERQAEEAKRRKKEKEKEKLQKKKL 60
Query: 390 EGKLLTGKQKEEQRRLEAMRRQIL-STGVATLP 485
EGKLLTGKQKEE RRLEAMRRQIL STG TLP
Sbjct: 61 EGKLLTGKQKEEARRLEAMRRQILNSTGGVTLP 93
>ref|NP_173540.1| translation initiation factor eIF-2, putative; protein id:
At1g21160.1 [Arabidopsis thaliana]
Length = 1088
Score = 136 bits (343), Expect = 1e-31
Identities = 76/141 (53%), Positives = 103/141 (72%)
Frame = +3
Query: 42 KKKKKEKEKEKKAAAAAAGSAPANETVEVKAEVVESKKNESKTKAADKKLPKHVREMQEA 221
KKKKK+K+K+KKA+A+ A S+ V+A+ K + K K A+KK+PKHVRE QE
Sbjct: 176 KKKKKKKDKDKKASASLAISS-------VEAKEDRQGKKDVKIKVAEKKVPKHVREKQET 228
Query: 222 LARRKEAEERKKREEEEKLRKEEEERRRQEELERQAEEARRRKKEREKEKLLKKKQEGKL 401
LAR KEAE+ KK+EEEE+LRKEEEERR +EE ER+AEE R+++K R+ E KKQEG +
Sbjct: 229 LARWKEAEDGKKKEEEERLRKEEEERRIEEEREREAEEIRQKRKIRKME----KKQEGLI 284
Query: 402 LTGKQKEEQRRLEAMRRQILS 464
LT KQK + + EA R+++L+
Sbjct: 285 LTAKQKRDAAKNEAFRKRVLT 305
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 411,407,205
Number of Sequences: 1393205
Number of extensions: 9492936
Number of successful extensions: 294956
Number of sequences better than 10.0: 15439
Number of HSP's better than 10.0 without gapping: 92110
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 187178
length of database: 448,689,247
effective HSP length: 113
effective length of database: 291,257,082
effective search space used: 13980339936
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
