Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC013816A_C01 KMC013816A_c01
(580 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAL35216.1| 4-coumarate:CoA ligase [Amorpha fruticosa] 191 4e-48
gb|AAC97600.1| 4-coumarate:CoA ligase isoenzyme 2 [Glycine max] 185 4e-46
sp|P31686|4CL1_SOYBN 4-coumarate--CoA ligase 1 (4CL 1) (4-coumar... 185 4e-46
sp|O24146|4CL2_TOBAC 4-coumarate--CoA ligase 2 (4CL 2) (4-coumar... 174 5e-43
sp|O24540|4CL_VANPL 4-coumarate--CoA ligase (4CL) (4-coumaroyl-C... 171 5e-42
>gb|AAL35216.1| 4-coumarate:CoA ligase [Amorpha fruticosa]
Length = 540
Score = 191 bits (486), Expect = 4e-48
Identities = 97/106 (91%), Positives = 101/106 (94%)
Frame = -2
Query: 579 KELILYKGFQVAPAELEALLLSHPKISDVAVVPMKDEAAGEVPVAFVVRSNGHTDINEDE 400
KELI YKGFQVAPAELEALLLSHPKI+D AVVPMKDEAAGEVPVAFVVRSNGHTD EDE
Sbjct: 434 KELIKYKGFQVAPAELEALLLSHPKITDAAVVPMKDEAAGEVPVAFVVRSNGHTDTTEDE 493
Query: 399 IKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKNLRSKLVAGVP 262
IKQFISKQVVFYKRI+RVFFIDAIPKSPSGKILRK+LR+KL AGVP
Sbjct: 494 IKQFISKQVVFYKRISRVFFIDAIPKSPSGKILRKDLRAKLAAGVP 539
>gb|AAC97600.1| 4-coumarate:CoA ligase isoenzyme 2 [Glycine max]
Length = 547
Score = 185 bits (469), Expect = 4e-46
Identities = 94/106 (88%), Positives = 99/106 (92%)
Frame = -2
Query: 579 KELILYKGFQVAPAELEALLLSHPKISDVAVVPMKDEAAGEVPVAFVVRSNGHTDINEDE 400
KELI YKGFQVAPAELEALLL+HPKISD AVVPMKDEAAGEVPVAFVV SNG+TD EDE
Sbjct: 441 KELIKYKGFQVAPAELEALLLTHPKISDAAVVPMKDEAAGEVPVAFVVISNGYTDTTEDE 500
Query: 399 IKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKNLRSKLVAGVP 262
IKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRK+LR+K+ A VP
Sbjct: 501 IKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKDLRAKIAASVP 546
>sp|P31686|4CL1_SOYBN 4-coumarate--CoA ligase 1 (4CL 1) (4-coumaroyl-CoA synthase 1)
(Clone 4CL14) gi|322689|pir||S31705 4-coumarate-CoA
ligase (EC 6.2.1.12) - soybean (fragment)
gi|18518|emb|CAA49575.1| 4-coumarate--CoA ligase
[Glycine max]
Length = 293
Score = 185 bits (469), Expect = 4e-46
Identities = 94/106 (88%), Positives = 99/106 (92%)
Frame = -2
Query: 579 KELILYKGFQVAPAELEALLLSHPKISDVAVVPMKDEAAGEVPVAFVVRSNGHTDINEDE 400
KELI YKGFQVAPAELEALLL+HPKISD AVVPMKDEAAGEVPVAFVV SNG+TD EDE
Sbjct: 187 KELIKYKGFQVAPAELEALLLTHPKISDAAVVPMKDEAAGEVPVAFVVISNGYTDTTEDE 246
Query: 399 IKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKNLRSKLVAGVP 262
IKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRK+LR+K+ A VP
Sbjct: 247 IKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKDLRAKIAASVP 292
>sp|O24146|4CL2_TOBAC 4-coumarate--CoA ligase 2 (4CL 2) (4-coumaroyl-CoA synthase 2)
gi|7437848|pir||T03789 4-coumarate-CoA ligase (EC
6.2.1.12) 4CL2 - common tobacco
gi|1663724|gb|AAB18638.1| 4-coumarate:coenzyme A ligase
[Nicotiana tabacum]
Length = 542
Score = 174 bits (442), Expect = 5e-43
Identities = 89/106 (83%), Positives = 96/106 (89%)
Frame = -2
Query: 579 KELILYKGFQVAPAELEALLLSHPKISDVAVVPMKDEAAGEVPVAFVVRSNGHTDINEDE 400
KELI YKGFQVAPAELEALLL+HP ISD AVVPMKDE AGEVPVAFVVRSNG T I EDE
Sbjct: 437 KELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVPVAFVVRSNGST-ITEDE 495
Query: 399 IKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKNLRSKLVAGVP 262
+K FISKQV+FYKRI RVFF+DAIPKSPSGKILRK+LR+KL AG+P
Sbjct: 496 VKDFISKQVIFYKRIKRVFFVDAIPKSPSGKILRKDLRAKLAAGLP 541
>sp|O24540|4CL_VANPL 4-coumarate--CoA ligase (4CL) (4-coumaroyl-CoA synthase)
Length = 553
Score = 171 bits (434), Expect = 5e-42
Identities = 87/103 (84%), Positives = 97/103 (93%)
Frame = -2
Query: 579 KELILYKGFQVAPAELEALLLSHPKISDVAVVPMKDEAAGEVPVAFVVRSNGHTDINEDE 400
KELI YKGFQVAPAELEALLL+HP ISD AVVPMKDEAAGEVPVAFVV+SNGH +I EDE
Sbjct: 446 KELIKYKGFQVAPAELEALLLTHPCISDAAVVPMKDEAAGEVPVAFVVKSNGH-NITEDE 504
Query: 399 IKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKNLRSKLVA 271
IKQFISKQV+FYKRINRVFF++AIPK+PSGKILRK+LR++L A
Sbjct: 505 IKQFISKQVIFYKRINRVFFVEAIPKAPSGKILRKDLRARLAA 547
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 466,565,969
Number of Sequences: 1393205
Number of extensions: 9645087
Number of successful extensions: 27102
Number of sequences better than 10.0: 1427
Number of HSP's better than 10.0 without gapping: 24930
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 26194
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 21426319650
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
