Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC012910A_C01 KMC012910A_c01
(683 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAF66825.1| poly(A)-binding protein [Nicotiana tabacum] 367 e-100
gb|AAK30205.1|AF349964_1 poly(A)-binding protein [Daucus carota] 350 1e-95
gb|AAF63202.1|AF240679_1 poly(A)-binding protein [Cucumis sativus] 347 7e-95
gb|AAF66823.1|AF190655_1 poly(A)-binding protein [Nicotiana taba... 346 1e-94
gb|AAF66824.1|AF190656_1 poly(A)-binding protein [Nicotiana taba... 344 7e-94
>gb|AAF66825.1| poly(A)-binding protein [Nicotiana tabacum]
Length = 479
Score = 367 bits (941), Expect = e-100
Identities = 179/219 (81%), Positives = 198/219 (89%), Gaps = 2/219 (0%)
Frame = +3
Query: 27 GTITSFKIMRDPTGISRGSGFVAFSTPEEASRALGEMNGKMISGKPLYVALAQRKEDRRA 206
GTITS K+MRDP+GISRGSGFVAFST EEASRAL EMNGKMI KPLYVALAQRKE+RRA
Sbjct: 166 GTITSCKVMRDPSGISRGSGFVAFSTSEEASRALSEMNGKMIVSKPLYVALAQRKEERRA 225
Query: 207 RLQAQFSQMRPVAISPSVAPRMPIYPPGAPGLGQQYLYGQGPPAMIPQQAGFGYQQQLMP 386
+L+AQFSQ+RPVA+ PS+APRMPIYPPGAPG+GQQ YGQGPPAMIP QAGFGYQQQL+P
Sbjct: 226 KLRAQFSQLRPVAMPPSLAPRMPIYPPGAPGIGQQLFYGQGPPAMIPPQAGFGYQQQLVP 285
Query: 387 GMRPGGAPMQSFFVPMVQQGQQGQRPGGRR-AGHVQQPQQPMPMMQQQMLPRGRVYRYPP 563
GMRPGGAPM +FF+P+VQQGQQGQRPGGRR AG VQQ QQPMP+MQQQMLPRGR+YRYPP
Sbjct: 286 GMRPGGAPMPNFFMPLVQQGQQGQRPGGRRGAGPVQQTQQPMPLMQQQMLPRGRMYRYPP 345
Query: 564 GRNMQDGPMQGVAGGMMSVPYDMGGLPIRD-PVGQPMPI 677
GRN +GPM GV GGM+SVPYDMGG+ RD +GQPMPI
Sbjct: 346 GRNGPEGPMPGVPGGMLSVPYDMGGMLPRDSAMGQPMPI 384
Score = 60.8 bits (146), Expect = 2e-08
Identities = 30/74 (40%), Positives = 44/74 (58%)
Frame = +3
Query: 27 GTITSFKIMRDPTGISRGSGFVAFSTPEEASRALGEMNGKMISGKPLYVALAQRKEDRRA 206
G ITS +MRD G S+ GF+ F T E+A++A+ +NGK K YV AQ+K +R
Sbjct: 63 GNITSAVVMRDADGKSKCFGFINFETAEDAAKAVESLNGKKFDDKEWYVGKAQKKSEREQ 122
Query: 207 RLQAQFSQMRPVAI 248
L+++F Q A+
Sbjct: 123 ELKSKFEQTAKEAV 136
>gb|AAK30205.1|AF349964_1 poly(A)-binding protein [Daucus carota]
Length = 658
Score = 350 bits (898), Expect = 1e-95
Identities = 172/221 (77%), Positives = 191/221 (85%), Gaps = 2/221 (0%)
Frame = +3
Query: 27 GTITSFKIMRDPTGISRGSGFVAFSTPEEASRALGEMNGKMISGKPLYVALAQRKEDRRA 206
GTITS K+MRDP+GISRGSGFVAFSTPEEASRALGEMNGKMI KPLYVALAQRKE+RRA
Sbjct: 346 GTITSCKVMRDPSGISRGSGFVAFSTPEEASRALGEMNGKMIVSKPLYVALAQRKEERRA 405
Query: 207 RLQAQFSQMRPVAISPSVAPRMPIYPPGAPGLGQQYLYGQGPPAMIPQQAGFGYQQQLMP 386
RLQAQFSQMRPVA+ PS+ PRMP+Y PGAPG+GQQ YGQ PPAMI QAGFGYQQQL+P
Sbjct: 406 RLQAQFSQMRPVAMPPSMGPRMPMYSPGAPGMGQQLFYGQAPPAMIAPQAGFGYQQQLVP 465
Query: 387 GMRPGGAPMQSFFVPMVQQGQQGQRPGGRR-AGHVQQPQQPMPMMQQQMLPRGRVYRYPP 563
GMRPGG PM +FF+PMVQQGQQGQRPGGRR AG QQ QQP+PMM QQ++PRGR+YR+PP
Sbjct: 466 GMRPGGGPMPNFFMPMVQQGQQGQRPGGRRGAGPAQQTQQPVPMMPQQLMPRGRMYRFPP 525
Query: 564 GRNMQDGPMQGVAGGMMSVPYDMGG-LPIRDPVGQPMPIQA 683
GRN+ D M GV GGM+ VPY+MGG LP +GQPMPI A
Sbjct: 526 GRNVGDVTMPGVGGGMLPVPYNMGGMLPREAAMGQPMPITA 566
Score = 64.3 bits (155), Expect = 1e-09
Identities = 31/68 (45%), Positives = 43/68 (62%)
Frame = +3
Query: 27 GTITSFKIMRDPTGISRGSGFVAFSTPEEASRALGEMNGKMISGKPLYVALAQRKEDRRA 206
GTITS +MRD G S+ GFV F PE+A++A+ +NGK K YV AQ+K +R
Sbjct: 243 GTITSVVVMRDADGKSKCFGFVNFENPEDAAKAVDALNGKKFDDKEWYVGKAQKKSEREV 302
Query: 207 RLQAQFSQ 230
L+++F Q
Sbjct: 303 ELKSRFEQ 310
Score = 53.1 bits (126), Expect = 3e-06
Identities = 25/62 (40%), Positives = 40/62 (64%)
Frame = +3
Query: 27 GTITSFKIMRDPTGISRGSGFVAFSTPEEASRALGEMNGKMISGKPLYVALAQRKEDRRA 206
GTI S KI D +G S+G GFV + + E A A+ ++NG +++ K +YV + RK+DR +
Sbjct: 152 GTIISCKIATDASGQSKGYGFVQYDSEEAAQTAIDKLNGMLMNDKQVYVGVFLRKQDRDS 211
Query: 207 RL 212
+
Sbjct: 212 EM 213
>gb|AAF63202.1|AF240679_1 poly(A)-binding protein [Cucumis sativus]
Length = 649
Score = 347 bits (891), Expect = 7e-95
Identities = 170/221 (76%), Positives = 190/221 (85%), Gaps = 2/221 (0%)
Frame = +3
Query: 27 GTITSFKIMRDPTGISRGSGFVAFSTPEEASRALGEMNGKMISGKPLYVALAQRKEDRRA 206
GTITS K+MRDP GISRGSGFVAFS+PEEA+RAL EMNG+MI KPLYVALAQRKEDR A
Sbjct: 335 GTITSCKVMRDPNGISRGSGFVAFSSPEEAARALAEMNGRMIVSKPLYVALAQRKEDRIA 394
Query: 207 RLQAQFSQMRPVAISPSVAPR-MPIYPPGAPGLGQQYLYGQGPPAMIPQQAGFGYQQQLM 383
RLQAQFSQM+P+A++ SVAPR MP+YPPG PG+GQQ YGQ PP +I Q GFGYQQQLM
Sbjct: 395 RLQAQFSQMQPMAMASSVAPRGMPMYPPGGPGIGQQIFYGQAPPTIISSQPGFGYQQQLM 454
Query: 384 PGMRPGGAPMQSFFVPMVQQGQQGQRPGGRRAGHVQQPQQPMPMMQQQMLPRGRVYRYPP 563
PGMRPGG PM +FFVPMVQQGQQGQR GGRRAG +QQ QQP+P+MQQQMLPRGRVYRYPP
Sbjct: 455 PGMRPGGGPMPNFFVPMVQQGQQGQRSGGRRAGAIQQTQQPVPLMQQQMLPRGRVYRYPP 514
Query: 564 GRNMQDGPMQGVAGGMMSVPYDMGGLPIRDPV-GQPMPIQA 683
GR + D PM GVAGGM SVPY+MGG+P RD V QP+P+ A
Sbjct: 515 GRGLPDLPMPGVAGGMFSVPYEMGGMPPRDAVHPQPVPVGA 555
Score = 60.8 bits (146), Expect = 2e-08
Identities = 29/68 (42%), Positives = 43/68 (62%)
Frame = +3
Query: 27 GTITSFKIMRDPTGISRGSGFVAFSTPEEASRALGEMNGKMISGKPLYVALAQRKEDRRA 206
G ITS +MRD G S+ GFV F ++A+R++ +NGK + GK YV AQ+K +R
Sbjct: 232 GPITSVVVMRDGEGKSKCFGFVNFENADDAARSVEALNGKKVDGKEWYVGKAQKKSEREV 291
Query: 207 RLQAQFSQ 230
L+++F Q
Sbjct: 292 ELKSRFEQ 299
Score = 49.7 bits (117), Expect = 4e-05
Identities = 23/60 (38%), Positives = 39/60 (64%)
Frame = +3
Query: 27 GTITSFKIMRDPTGISRGSGFVAFSTPEEASRALGEMNGKMISGKPLYVALAQRKEDRRA 206
G+I S K+ D +G S+G GFV F T E A +A+ ++NG +++ K ++V RK++R +
Sbjct: 141 GSILSCKVATDSSGQSKGFGFVQFDTEEAALKAIEKLNGMLLNDKQVFVGPFLRKQERES 200
Score = 40.0 bits (92), Expect = 0.030
Identities = 22/56 (39%), Positives = 33/56 (58%), Gaps = 1/56 (1%)
Frame = +3
Query: 24 VGTITSFKIMRDPTGI-SRGSGFVAFSTPEEASRALGEMNGKMISGKPLYVALAQR 188
VG + S ++ RD T S G G+V +S P +ASRAL +N ++G P+ V + R
Sbjct: 52 VGQVVSVRVCRDLTSRRSLGYGYVNYSNPVDASRALDVLNFTPLNGNPIRVMYSHR 107
>gb|AAF66823.1|AF190655_1 poly(A)-binding protein [Nicotiana tabacum]
Length = 649
Score = 346 bits (888), Expect = 1e-94
Identities = 166/222 (74%), Positives = 189/222 (84%), Gaps = 3/222 (1%)
Frame = +3
Query: 27 GTITSFKIMRDPTGISRGSGFVAFSTPEEASRALGEMNGKMISGKPLYVALAQRKEDRRA 206
GTITS K+MRDP+G+S+GSGFVAFS PEEASRAL EMNGKM+ KPLYVALAQRKE+RRA
Sbjct: 333 GTITSCKVMRDPSGVSKGSGFVAFSNPEEASRALSEMNGKMVVSKPLYVALAQRKEERRA 392
Query: 207 RLQAQFSQMRPVAISPSVAPRMPIYPPGAPGLGQQYLYGQGPPAMIPQQAGFGYQQQLMP 386
RLQAQFSQMRP+A++ SVAPRMP+YPPG PGLGQQ YGQ PAM+P QAGFGYQQQL+P
Sbjct: 393 RLQAQFSQMRPIAMASSVAPRMPMYPPGGPGLGQQIFYGQPQPAMLPPQAGFGYQQQLVP 452
Query: 387 GMRPGGAPMQSFFVPMVQQGQQGQRPGGRRAGHV--QQPQQPMPMMQQQMLPRGRVYRYP 560
GMRPGG PM +FF+PMVQQGQQGQRPGGRR G V QQ QQP+P+MQQQM PRGR YRYP
Sbjct: 453 GMRPGGGPMPNFFMPMVQQGQQGQRPGGRRGGAVPLQQGQQPVPLMQQQMFPRGRGYRYP 512
Query: 561 PGRNMQDGPMQGVAGGMMSVPYDMGGLPIRDP-VGQPMPIQA 683
PGR + D GV GGM S+PYDMGG+P+RD + QP+P+ A
Sbjct: 513 PGRGLPDVAFPGVGGGMFSIPYDMGGMPVRDAGIVQPIPVGA 554
Score = 57.8 bits (138), Expect = 1e-07
Identities = 30/77 (38%), Positives = 43/77 (54%)
Frame = +3
Query: 27 GTITSFKIMRDPTGISRGSGFVAFSTPEEASRALGEMNGKMISGKPLYVALAQRKEDRRA 206
G ITS +M+D G SR GFV F E+A+RA+ +NG + K +V AQ+K +R
Sbjct: 230 GAITSVAVMKDEDGKSRCFGFVNFENAEDAARAVEALNGYKLDNKDWFVGRAQKKSEREM 289
Query: 207 RLQAQFSQMRPVAISPS 257
L+ +F Q A+ S
Sbjct: 290 ELKHRFEQSAKEAVDKS 306
Score = 47.0 bits (110), Expect = 2e-04
Identities = 22/58 (37%), Positives = 36/58 (61%)
Frame = +3
Query: 27 GTITSFKIMRDPTGISRGSGFVAFSTPEEASRALGEMNGKMISGKPLYVALAQRKEDR 200
G I S K+ D +G S+G GFV + + E A +A+ ++NG +++ K +YV RK +R
Sbjct: 139 GNILSCKVAVDSSGQSKGYGFVQYDSEEAAQKAIEKLNGMLLNDKQVYVGPFVRKHER 196
Score = 38.1 bits (87), Expect = 0.11
Identities = 19/56 (33%), Positives = 33/56 (58%), Gaps = 1/56 (1%)
Frame = +3
Query: 24 VGTITSFKIMRDPTGI-SRGSGFVAFSTPEEASRALGEMNGKMISGKPLYVALAQR 188
+G + S ++ RD T S G G+V + P++A+RAL +N + GKP+ + + R
Sbjct: 50 LGQVVSVRVCRDLTSQRSLGYGYVNYGNPQDAARALEVLNFTPLHGKPIRIMYSNR 105
>gb|AAF66824.1|AF190656_1 poly(A)-binding protein [Nicotiana tabacum]
Length = 330
Score = 344 bits (882), Expect = 7e-94
Identities = 165/222 (74%), Positives = 188/222 (84%), Gaps = 3/222 (1%)
Frame = +3
Query: 27 GTITSFKIMRDPTGISRGSGFVAFSTPEEASRALGEMNGKMISGKPLYVALAQRKEDRRA 206
GTITS K+MRDP+G+S+GSGFVAFS PEEASRAL EMNGKM+ KPLYVALAQRKE+RRA
Sbjct: 14 GTITSCKVMRDPSGVSKGSGFVAFSNPEEASRALSEMNGKMVVSKPLYVALAQRKEERRA 73
Query: 207 RLQAQFSQMRPVAISPSVAPRMPIYPPGAPGLGQQYLYGQGPPAMIPQQAGFGYQQQLMP 386
RLQAQFSQMRP+A++ SVAPRMP+YPPG PGLGQQ YGQ PAM+P QAGFGYQQQL+P
Sbjct: 74 RLQAQFSQMRPIAMASSVAPRMPMYPPGGPGLGQQIFYGQPQPAMLPPQAGFGYQQQLVP 133
Query: 387 GMRPGGAPMQSFFVPMVQQGQQGQRPGGRRAGHV--QQPQQPMPMMQQQMLPRGRVYRYP 560
GMRPGG PM +FF+PMVQQGQQGQRPG RR G V QQ QQP+P+MQQQM PRGR YRYP
Sbjct: 134 GMRPGGGPMPNFFMPMVQQGQQGQRPGSRRGGAVPLQQGQQPVPLMQQQMFPRGRGYRYP 193
Query: 561 PGRNMQDGPMQGVAGGMMSVPYDMGGLPIRDP-VGQPMPIQA 683
PGR + D GV GGM S+PYDMGG+P+RD + QP+P+ A
Sbjct: 194 PGRGLPDVAFPGVGGGMFSIPYDMGGMPVRDAGIVQPIPVGA 235
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 702,269,773
Number of Sequences: 1393205
Number of extensions: 18657411
Number of successful extensions: 72792
Number of sequences better than 10.0: 2032
Number of HSP's better than 10.0 without gapping: 59117
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 69404
length of database: 448,689,247
effective HSP length: 119
effective length of database: 282,897,852
effective search space used: 30552968016
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
