Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC011914A_C01 KMC011914A_c01
(606 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
dbj|BAC16007.1| putative pre-mRNA splicing factor SF2 (SR1 prote... 155 1e-54
dbj|BAB90350.1| putative pre-mRNA splicing factor SF2 [Oryza sat... 157 1e-54
ref|NP_567235.2| arginine/serine-rich splicing factor (atSRp34),... 160 7e-54
pir||T01307 alternative splicing factor ASF-2 homolog T14P8.21 -... 157 4e-53
ref|NP_683270.1| pre-mRNA splicing factor SF2 (SR1 protein); pro... 156 7e-53
>dbj|BAC16007.1| putative pre-mRNA splicing factor SF2 (SR1 protein) [Oryza sativa
(japonica cultivar-group)]
Length = 380
Score = 155 bits (393), Expect(2) = 1e-54
Identities = 77/106 (72%), Positives = 90/106 (84%), Gaps = 2/106 (1%)
Frame = +1
Query: 295 GAIGYDFDGHRLRVELAHGGRGNSSSRDRYSSHSSGRGGR--GVSRRSEYRVLVSGLPSS 468
G GY+FDG+RLRVELAHGGRGNSSS ++SG GGR GVSR +EYRVLV+GLPSS
Sbjct: 130 GRDGYNFDGNRLRVELAHGGRGNSSS-----FNNSGGGGRRGGVSRHTEYRVLVTGLPSS 184
Query: 469 ASWQDLKDHMRKAGDVCFSQVFHDGRGTTGIVDYTNHDDMKYAVKK 606
ASWQDLKDHMR AGDVC+S+V+ +G GT GIVDYTN+DDMKYA++K
Sbjct: 185 ASWQDLKDHMRNAGDVCYSEVYREGGGTIGIVDYTNYDDMKYAIRK 230
Score = 79.7 bits (195), Expect(2) = 1e-54
Identities = 37/46 (80%), Positives = 40/46 (86%)
Frame = +3
Query: 84 EKKMSRRTSRTVYVGNLAGDIREREVEDLFYKYGNITHIDLKVPPR 221
E +MSRR SRT+YVGNL GDIREREVEDLFYKYG I IDLK+PPR
Sbjct: 64 EGRMSRRWSRTIYVGNLPGDIREREVEDLFYKYGRIVDIDLKIPPR 109
>dbj|BAB90350.1| putative pre-mRNA splicing factor SF2 [Oryza sativa (japonica
cultivar-group)]
Length = 250
Score = 157 bits (396), Expect(2) = 1e-54
Identities = 76/104 (73%), Positives = 88/104 (84%)
Frame = +1
Query: 295 GAIGYDFDGHRLRVELAHGGRGNSSSRDRYSSHSSGRGGRGVSRRSEYRVLVSGLPSSAS 474
G GY+FDG+RLRVELAHGGRG S S DR S+SSGR G GVSRRSEYRV+V+GLPSSAS
Sbjct: 62 GRDGYNFDGYRLRVELAHGGRGQSYSYDRPRSYSSGRRG-GVSRRSEYRVMVTGLPSSAS 120
Query: 475 WQDLKDHMRKAGDVCFSQVFHDGRGTTGIVDYTNHDDMKYAVKK 606
WQDLKDHMR+AGDVCFS V+ + T GIVDYT ++DMKYA++K
Sbjct: 121 WQDLKDHMRRAGDVCFSDVYREAGATVGIVDYTTYEDMKYAIRK 164
Score = 78.2 bits (191), Expect(2) = 1e-54
Identities = 36/43 (83%), Positives = 38/43 (87%)
Frame = +3
Query: 93 MSRRTSRTVYVGNLAGDIREREVEDLFYKYGNITHIDLKVPPR 221
MSRR SRT+YVGNL GDIREREVEDLFYKYG I IDLK+PPR
Sbjct: 1 MSRRNSRTIYVGNLPGDIREREVEDLFYKYGRILDIDLKIPPR 43
>ref|NP_567235.2| arginine/serine-rich splicing factor (atSRp34), putative; protein
id: At4g02430.1 [Arabidopsis thaliana]
gi|26451203|dbj|BAC42705.1| unknown protein [Arabidopsis
thaliana]
Length = 178
Score = 160 bits (405), Expect(2) = 7e-54
Identities = 82/114 (71%), Positives = 91/114 (78%), Gaps = 9/114 (7%)
Frame = +1
Query: 292 WGAIGYDFDGHRLRVELAHGGRGNS-SSRDRYSSHS-SGRGG-------RGVSRRSEYRV 444
+G GYDFDGH LRVELAHGGR +S +R YS GRGG RG SRRSEYRV
Sbjct: 63 YGRDGYDFDGHHLRVELAHGGRRSSHDARGSYSGRGRGGRGGGDGGGRERGPSRRSEYRV 122
Query: 445 LVSGLPSSASWQDLKDHMRKAGDVCFSQVFHDGRGTTGIVDYTNHDDMKYAVKK 606
+VSGLPSSASWQDLKDHMRK G+VCFSQVF DGRGTTGIVDYT+++DMKYA+KK
Sbjct: 123 VVSGLPSSASWQDLKDHMRKGGEVCFSQVFRDGRGTTGIVDYTSYEDMKYAIKK 176
Score = 72.4 bits (176), Expect(2) = 7e-54
Identities = 33/43 (76%), Positives = 37/43 (85%)
Frame = +3
Query: 93 MSRRTSRTVYVGNLAGDIREREVEDLFYKYGNITHIDLKVPPR 221
MS R+SRT+YVGNL GDIREREVEDLF KYG + IDLK+PPR
Sbjct: 1 MSSRSSRTIYVGNLPGDIREREVEDLFSKYGPVVQIDLKIPPR 43
>pir||T01307 alternative splicing factor ASF-2 homolog T14P8.21 - Arabidopsis
thaliana gi|3193304|gb|AAC19288.1| contains similarity
to RNA recognition motifs (Pfam: rrm.hmm, score; 82.09
and 30.80); similar to alternative splicing factor ASF
[Arabidopsis thaliana] gi|7269003|emb|CAB80736.1|
contains similarity to RNA recognition motifs (Pfam:
rrm.hmm, score, 82.09 and 30.80)~strong similarity to
similar to alternative splicing factor ASF~contains EST
gb:Z25964.1 [Arabidopsis thaliana]
Length = 294
Score = 157 bits (398), Expect(2) = 4e-53
Identities = 81/113 (71%), Positives = 90/113 (78%), Gaps = 9/113 (7%)
Frame = +1
Query: 292 WGAIGYDFDGHRLRVELAHGGRGNS-SSRDRYSSHS-SGRGG-------RGVSRRSEYRV 444
+G GYDFDGH LRVELAHGGR +S +R YS GRGG RG SRRSEYRV
Sbjct: 63 YGRDGYDFDGHHLRVELAHGGRRSSHDARGSYSGRGRGGRGGGDGGGRERGPSRRSEYRV 122
Query: 445 LVSGLPSSASWQDLKDHMRKAGDVCFSQVFHDGRGTTGIVDYTNHDDMKYAVK 603
+VSGLPSSASWQDLKDHMRK G+VCFSQVF DGRGTTGIVDYT+++DMKYAV+
Sbjct: 123 VVSGLPSSASWQDLKDHMRKGGEVCFSQVFRDGRGTTGIVDYTSYEDMKYAVR 175
Score = 72.4 bits (176), Expect(2) = 4e-53
Identities = 33/43 (76%), Positives = 37/43 (85%)
Frame = +3
Query: 93 MSRRTSRTVYVGNLAGDIREREVEDLFYKYGNITHIDLKVPPR 221
MS R+SRT+YVGNL GDIREREVEDLF KYG + IDLK+PPR
Sbjct: 1 MSSRSSRTIYVGNLPGDIREREVEDLFSKYGPVVQIDLKIPPR 43
>ref|NP_683270.1| pre-mRNA splicing factor SF2 (SR1 protein); protein id:
At1g02840.2, supported by cDNA: 19681., supported by
cDNA: gi_166843 [Arabidopsis thaliana]
gi|3929368|sp|O22315|SFR1_ARATH Pre-mRNA splicing factor
SF2 (SR1 protein) gi|25386574|pir||F86158 alternative
splicing factor SF2a [imported] - Arabidopsis thaliana
gi|2443472|gb|AAB71385.1| ASF/SF2 homolog [Arabidopsis
thaliana] gi|6056417|gb|AAF02881.1|AC009525_15
alternative splicing factor SF2a [Arabidopsis thaliana]
gi|21554051|gb|AAM63132.1| SF2/ASF-like splicing
modulator Srp30, putative [Arabidopsis thaliana]
gi|26451794|dbj|BAC42991.1| putative ribonucleoprotein
SF-2 [Arabidopsis thaliana]
Length = 303
Score = 156 bits (394), Expect(2) = 7e-53
Identities = 80/113 (70%), Positives = 88/113 (77%), Gaps = 9/113 (7%)
Frame = +1
Query: 295 GAIGYDFDGHRLRVELAHGGRGNSSSRDRYSSHSSGRGG---------RGVSRRSEYRVL 447
G GYDFDGHRLRVELAHGGR SS R S + GRGG RG SRRSE+RVL
Sbjct: 64 GRDGYDFDGHRLRVELAHGGR-RSSDDTRGSFNGGGRGGGRGRGDGGSRGPSRRSEFRVL 122
Query: 448 VSGLPSSASWQDLKDHMRKAGDVCFSQVFHDGRGTTGIVDYTNHDDMKYAVKK 606
V+GLPSSASWQDLKDHMRK GDVCFSQV+ D RGTTG+VDYT ++DMKYA+KK
Sbjct: 123 VTGLPSSASWQDLKDHMRKGGDVCFSQVYRDARGTTGVVDYTCYEDMKYALKK 175
Score = 73.2 bits (178), Expect(2) = 7e-53
Identities = 35/43 (81%), Positives = 37/43 (85%)
Frame = +3
Query: 93 MSRRTSRTVYVGNLAGDIREREVEDLFYKYGNITHIDLKVPPR 221
MS R+SRTVYVGNL GDIREREVEDLF KYG + IDLKVPPR
Sbjct: 1 MSSRSSRTVYVGNLPGDIREREVEDLFSKYGPVVQIDLKVPPR 43
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 573,447,589
Number of Sequences: 1393205
Number of extensions: 13166357
Number of successful extensions: 42855
Number of sequences better than 10.0: 356
Number of HSP's better than 10.0 without gapping: 39307
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 42536
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 23997478008
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
