Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC010351A_C01 KMC010351A_c01
(569 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_178207.1| J8-like protein; protein id: At1g80920.1, suppo... 174 9e-43
pir||T51864 probable heat-shock protein J8 [imported] - Arabidop... 147 9e-35
ref|NP_565034.1| DnaJ protein, putative; protein id: At1g72070.1... 66 3e-10
gb|AAM63520.1| DnaJ protein, putative [Arabidopsis thaliana] 65 6e-10
ref|NP_746953.1| curved-DNA-binding protein, DnaJ family [Pseudo... 58 1e-07
>ref|NP_178207.1| J8-like protein; protein id: At1g80920.1, supported by cDNA: 4150.,
supported by cDNA: gi_12083329, supported by cDNA:
gi_14532785, supported by cDNA: gi_19310738 [Arabidopsis
thaliana] gi|25406705|pir||B96842 hypothetical protein
F23A5.28 [imported] - Arabidopsis thaliana
gi|6503304|gb|AAF14680.1|AC011713_28 Strong similarity
to gb|AF099906 J8 gene from Arabidopsis thaliana and
contains PF|00226 DnaJ domain. ESTs gb|T46318,
gb|Z27035, gb|T76034, gb|AA394916 and gb|AI995985 come
from this gene gi|12083330|gb|AAG48824.1|AF332461_1
putative J8 protein [Arabidopsis thaliana]
gi|14532786|gb|AAK64174.1| putative J8 protein
[Arabidopsis thaliana] gi|19310739|gb|AAL85100.1|
putative J8 protein [Arabidopsis thaliana]
gi|21593700|gb|AAM65667.1| J8-like protein [Arabidopsis
thaliana]
Length = 163
Score = 174 bits (440), Expect = 9e-43
Identities = 79/119 (66%), Positives = 94/119 (78%)
Frame = -2
Query: 568 VSCSYSSSVMDPYKTLKIQPGASETEVRKAFRQLALQYHPDVCRGSNCGVQFHLINEAYD 389
V CS SSSVMDPYKTLKI+P +SE EV+KAFRQLA +YHPDVCRGSNCGVQF INEAYD
Sbjct: 45 VVCSSSSSVMDPYKTLKIRPDSSEYEVKKAFRQLAKKYHPDVCRGSNCGVQFQTINEAYD 104
Query: 388 VVMANLRGESNEAETYGAYEVVTDEPFRGMHDPDYDMWEEWMGWEGAGIRDYTSHINPY 212
+V+ ++ + E + ++V DE GM+DPD D WEEWMGWEGAG RDY+SH+NPY
Sbjct: 105 IVLKQIKNQMEGTEEFEPFDVY-DEGLNGMNDPDCDTWEEWMGWEGAGTRDYSSHVNPY 162
>pir||T51864 probable heat-shock protein J8 [imported] - Arabidopsis thaliana
gi|3851670|gb|AAC72399.1| J8 [Arabidopsis thaliana]
Length = 109
Score = 147 bits (371), Expect = 9e-35
Identities = 69/110 (62%), Positives = 79/110 (71%)
Frame = -2
Query: 541 MDPYKTLKIQPGASETEVRKAFRQLALQYHPDVCRGSNCGVQFHLINEAYDVVMANLRGE 362
MDPYKTLKI+P +SE EV+KAFRQLA +YHPDVCRGSNCGVQF INEAYD+V+ L+
Sbjct: 1 MDPYKTLKIRPDSSEYEVKKAFRQLAKKYHPDVCRGSNCGVQFQTINEAYDIVLKQLKIR 60
Query: 361 SNEAETYGAYEVVTDEPFRGMHDPDYDMWEEWMGWEGAGIRDYTSHINPY 212
T M+DPD D WEEWMGWEGAG RDY+SH+NPY
Sbjct: 61 WKGRRNLSRSMYTTG--IERMNDPDCDTWEEWMGWEGAGTRDYSSHVNPY 108
>ref|NP_565034.1| DnaJ protein, putative; protein id: At1g72070.1, supported by cDNA:
25569. [Arabidopsis thaliana]
Length = 126
Score = 65.9 bits (159), Expect = 3e-10
Identities = 37/94 (39%), Positives = 51/94 (53%)
Frame = -2
Query: 532 YKTLKIQPGASETEVRKAFRQLALQYHPDVCRGSNCGVQFHLINEAYDVVMANLRGESNE 353
Y L + P AS+TEV++AF++LAL+YHPDV +G + F I AY+ +M + E E
Sbjct: 41 YTVLGLTPLASQTEVKRAFKRLALKYHPDVHKGQD--KDFKEIKSAYECLMQKFKKEEEE 98
Query: 352 AETYGAYEVVTDEPFRGMHDPDYDMWEEWMGWEG 251
E E+ D WEEWMG+EG
Sbjct: 99 MEITEMGEI--------------DEWEEWMGFEG 118
>gb|AAM63520.1| DnaJ protein, putative [Arabidopsis thaliana]
Length = 126
Score = 65.1 bits (157), Expect = 6e-10
Identities = 37/94 (39%), Positives = 50/94 (52%)
Frame = -2
Query: 532 YKTLKIQPGASETEVRKAFRQLALQYHPDVCRGSNCGVQFHLINEAYDVVMANLRGESNE 353
Y L + P AS+TEV++AF++LAL+YHPDV +G + F I AY+ +M E E
Sbjct: 41 YTVLGLTPLASQTEVKRAFKRLALKYHPDVHKGQD--KDFKEIKSAYECLMQKFEKEEEE 98
Query: 352 AETYGAYEVVTDEPFRGMHDPDYDMWEEWMGWEG 251
E E+ D WEEWMG+EG
Sbjct: 99 MEITEMGEI--------------DEWEEWMGFEG 118
>ref|NP_746953.1| curved-DNA-binding protein, DnaJ family [Pseudomonas putida KT2440]
gi|24986611|gb|AAN70417.1|AE016683_8 curved-DNA-binding
protein, DnaJ family [Pseudomonas putida KT2440]
Length = 319
Score = 57.8 bits (138), Expect = 1e-07
Identities = 37/107 (34%), Positives = 55/107 (50%), Gaps = 1/107 (0%)
Frame = -2
Query: 538 DPYKTLKIQPGASETEVRKAFRQLALQYHPDVCRGSNCGVQFHLINEAYDVV-MANLRGE 362
D YK L ++P A E ++ A+R+LA +YHPDV + + +F NEAY+V+ A R E
Sbjct: 5 DYYKILGVEPTADEKAIKAAYRKLARKYHPDVSKERDAEEKFKEANEAYEVLGDAQKRAE 64
Query: 361 SNEAETYGAYEVVTDEPFRGMHDPDYDMWEEWMGWEGAGIRDYTSHI 221
+E YG PF+ P ++ G+EG D+ S I
Sbjct: 65 FDEIRKYGGQH---GRPFQA--PPGWESRGGGGGFEGGDFSDFFSSI 106
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 486,207,187
Number of Sequences: 1393205
Number of extensions: 10245173
Number of successful extensions: 23473
Number of sequences better than 10.0: 1003
Number of HSP's better than 10.0 without gapping: 22747
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 23421
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 20956655091
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)