KMC010032A_c01
[Fasta Sequence]   [Nr Search]   [EST assemble image]  

Fasta Sequence
>KMC010032A_C01 KMC010032A_c01
gataaagtggagggcctaggcccaggaaaacagaaGGAAAGCTAGACTAGCCTGGGGACA
GATGTCCCCATTACATCATCATTAAGCAGAGGAAACAAAAAAGGTGGGGCATCTTCACTA
TTGTTTTATATACAATAATGTCAGGGGATTCATATAGACTCACTATTGTTATGGATTGTT
GTTATTGGACTTTTAGGGAATCAAATTTGAAAATATTAGACATTTCTGCAAAACATAGAC
GTTATCACGTTACCTTACCAATGGCATATCTTAATGTGATAGAAAGATGATAAGGTTGTT
TAATGTTTTGGCAGAGATGACAAGTGTGGGGGACCATATGAAGAGCATCCCAAGAGAGAA
CACATCTGTGGCCGTCCACTTGGACTGTGCTTCAACATGCAATCTGGCGAGCTTTACGTT
GCTGATGCTTACATGGGCCTTGTTGTTGTTGAGCCCAATGGGGGCACTGCTTAACCTTTA
CGTCCCTAAGAAGTGAGAAACAACAAGATTATCTGAAACCTTGAGGAACTCAGGTCCCTT
ATGCTCCCTTTGGGAGAGGTCAACATGCACGGCTTCAAACTCAATTTCCTTCTCAATCAT
ACACATAACCACATCTCTGGGAGCAGCATAGGCAAATAACCTGTATTGTCCTTGTCAAAT
ACAAGGTAATCAAGTACTTCCCTTTTGAGCTGGGTGACATAATCTTAAGCAATAGAATTT
AGTAGGATGATGCTATTCACCAATTCAAATTGACTATTTTTAGATATCTTCAGACCCAAA
ATTTGACACATAGGCCACTT


Nr search

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC010032A_C01 KMC010032A_c01
         (800 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_181661.1| strictosidine synthase-related; protein id: At2...    71  2e-11
gb|AAC27642.1| putative strictosidine synthase [Arabidopsis thal...    71  2e-11
gb|AAF75751.1|AF261141_1 putative strictosidine synthase [Lycope...    69  6e-11
ref|NP_191262.1| strictosidine synthase-related; protein id: At3...    66  7e-10
ref|NP_680189.1| similar to strictosidine synthase-related; prot...    64  3e-09

>ref|NP_181661.1| strictosidine synthase-related; protein id: At2g41290.1
           [Arabidopsis thaliana] gi|25335890|pir||A84840 probable
           strictosidine synthase [imported] - Arabidopsis thaliana
           gi|3894194|gb|AAC78543.1| putative strictosidine
           synthase [Arabidopsis thaliana]
          Length = 376

 Score = 70.9 bits (172), Expect = 2e-11
 Identities = 32/57 (56%), Positives = 42/57 (73%)
 Frame = +1

Query: 325 CGGPYEEHPKREHICGRPLGLCFNMQSGELYVADAYMGLVVVEPNGGTA*PLRP*EV 495
           C GP+E H + EH+CGRPLGL F+  +G+LY+ADAYMGL+ V P GG A  + P E+
Sbjct: 96  CEGPHE-HQRTEHVCGRPLGLAFDKSTGDLYIADAYMGLLKVGPTGGVATQVLPREL 151

>gb|AAC27642.1| putative strictosidine synthase [Arabidopsis thaliana]
          Length = 376

 Score = 70.9 bits (172), Expect = 2e-11
 Identities = 32/57 (56%), Positives = 42/57 (73%)
 Frame = +1

Query: 325 CGGPYEEHPKREHICGRPLGLCFNMQSGELYVADAYMGLVVVEPNGGTA*PLRP*EV 495
           C GP+E H + EH+CGRPLGL F+  +G+LY+ADAYMGL+ V P GG A  + P E+
Sbjct: 96  CEGPHE-HQRTEHVCGRPLGLAFDKSTGDLYIADAYMGLLKVGPTGGVANQVLPREL 151

>gb|AAF75751.1|AF261141_1 putative strictosidine synthase [Lycopersicon esculentum]
          Length = 351

 Score = 69.3 bits (168), Expect = 6e-11
 Identities = 33/52 (63%), Positives = 39/52 (74%)
 Frame = +1

Query: 325 CGGPYEEHPKREHICGRPLGLCFNMQSGELYVADAYMGLVVVEPNGGTA*PL 480
           C  P+   PK EHICGRPLGL F+ ++GELY+ADAY+GL VV P GG A PL
Sbjct: 90  CTSPFA--PKMEHICGRPLGLRFDTKTGELYIADAYLGLQVVGPKGGLATPL 139

>ref|NP_191262.1| strictosidine synthase-related; protein id: At3g57030.1
           [Arabidopsis thaliana] gi|11280567|pir||T47763
           hypothetical protein F24I3.110 - Arabidopsis thaliana
           gi|6911873|emb|CAB72173.1| putative protein [Arabidopsis
           thaliana]
          Length = 372

 Score = 65.9 bits (159), Expect = 7e-10
 Identities = 30/53 (56%), Positives = 40/53 (74%)
 Frame = +1

Query: 322 KCGGPYEEHPKREHICGRPLGLCFNMQSGELYVADAYMGLVVVEPNGGTA*PL 480
           +C  P+   P+ EH+CGRPLGL F+ ++G+LY+ADAY GL+VV P GG A PL
Sbjct: 98  ECARPFA--PELEHVCGRPLGLRFDKKTGDLYIADAYFGLLVVGPAGGLAKPL 148

>ref|NP_680189.1| similar to strictosidine synthase-related; protein id: At5g22020.1
           [Arabidopsis thaliana] gi|13374861|emb|CAC34495.1|
           putative strictosidine synthase-like [Arabidopsis
           thaliana]
          Length = 395

 Score = 63.5 bits (153), Expect = 3e-09
 Identities = 28/43 (65%), Positives = 35/43 (81%)
 Frame = +1

Query: 352 KREHICGRPLGLCFNMQSGELYVADAYMGLVVVEPNGGTA*PL 480
           + EHICGRPLGL F+ ++G+LY+ADAYMGL+ V P GG A PL
Sbjct: 130 RNEHICGRPLGLRFDKRTGDLYIADAYMGLLKVGPEGGLATPL 172

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 704,105,502
Number of Sequences: 1393205
Number of extensions: 15805427
Number of successful extensions: 32430
Number of sequences better than 10.0: 96
Number of HSP's better than 10.0 without gapping: 31283
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 32415
length of database: 448,689,247
effective HSP length: 121
effective length of database: 280,111,442
effective search space used: 40616159090
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)


EST assemble image


clone accession position
1 MR047a09_f BP079606 1 318
2 MFBL043b07_f BP043426 36 540
3 SPDL068g05_f BP056239 307 800




Lotus japonicus
Kazusa DNA Research Institute