Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC009608A_C01 KMC009608A_c01
(476 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAM65052.1| membrane associated protein-like protein [Arabido... 110 5e-30
ref|NP_567287.1| contains similarity to Arabidopsis thaliana mem... 110 5e-30
ref|NP_567627.1| putative membrane associated protein; protein i... 114 2e-29
pir||T05153 hypothetical protein F18E5.70 - Arabidopsis thaliana... 114 2e-29
dbj|BAA97133.1| membrane associated protein [Arabidopsis thaliana] 95 4e-19
>gb|AAM65052.1| membrane associated protein-like protein [Arabidopsis thaliana]
Length = 287
Score = 110 bits (275), Expect(2) = 5e-30
Identities = 59/89 (66%), Positives = 68/89 (76%), Gaps = 8/89 (8%)
Frame = +2
Query: 233 HLHHHHHHNPN---LHHPPPLEGSTASH-----TSGSVSSVARSLLPTRRRLKLDPSSKL 388
HL+ ++ H H PP +EG +H T S+SSVARSLLPT+RRLKLDPS+KL
Sbjct: 49 HLNQNYIHQSRHFQYHGPPVVEGLGQNHHQSAATIPSMSSVARSLLPTKRRLKLDPSAKL 108
Query: 389 YFPYEPGKQVRSAIRIKNTSKSHVAFKFQ 475
YFPYEPGKQVRSAI+IKNTSKSHVAFKFQ
Sbjct: 109 YFPYEPGKQVRSAIKIKNTSKSHVAFKFQ 137
Score = 41.6 bits (96), Expect(2) = 5e-30
Identities = 20/43 (46%), Positives = 29/43 (66%)
Frame = +1
Query: 115 MAVAEPKPHSEPKVWNLFKLPFRHSSTTANSTGTTSSSSTPSS 243
MA+ E K S+ + W FKLPFR+S++ A S ++SS +T SS
Sbjct: 1 MALTEDKSDSDGRRWGKFKLPFRNSNSQAPSASSSSSMATSSS 43
>ref|NP_567287.1| contains similarity to Arabidopsis thaliana membrane associated
protein (GB:U83655); protein id: At4g05060.1, supported
by cDNA: 36198., supported by cDNA: gi_20453192
gi|20453193|gb|AAM19836.1| AT4g05060/T32N4_12
[Arabidopsis thaliana] gi|23505843|gb|AAN28781.1|
At4g05060/T32N4_12 [Arabidopsis thaliana]
Length = 287
Score = 110 bits (275), Expect(2) = 5e-30
Identities = 59/89 (66%), Positives = 68/89 (76%), Gaps = 8/89 (8%)
Frame = +2
Query: 233 HLHHHHHHNPN---LHHPPPLEGSTASH-----TSGSVSSVARSLLPTRRRLKLDPSSKL 388
HL+ ++ H H PP +EG +H T S+SSVARSLLPT+RRLKLDPS+KL
Sbjct: 49 HLNQNYIHQSRHFQYHGPPVVEGLGQNHHQSAATIPSMSSVARSLLPTKRRLKLDPSAKL 108
Query: 389 YFPYEPGKQVRSAIRIKNTSKSHVAFKFQ 475
YFPYEPGKQVRSAI+IKNTSKSHVAFKFQ
Sbjct: 109 YFPYEPGKQVRSAIKIKNTSKSHVAFKFQ 137
Score = 41.6 bits (96), Expect(2) = 5e-30
Identities = 20/43 (46%), Positives = 29/43 (66%)
Frame = +1
Query: 115 MAVAEPKPHSEPKVWNLFKLPFRHSSTTANSTGTTSSSSTPSS 243
MA+ E K S+ + W FKLPFR+S++ A S ++SS +T SS
Sbjct: 1 MALTEDKSDSDGRRWGKFKLPFRNSNSQAPSASSSSSMATSSS 43
>ref|NP_567627.1| putative membrane associated protein; protein id: At4g21450.1,
supported by cDNA: 33975., supported by cDNA:
gi_17979372, supported by cDNA: gi_20465772 [Arabidopsis
thaliana] gi|17979373|gb|AAL49912.1| putative membrane
associated protein [Arabidopsis thaliana]
gi|20465773|gb|AAM20375.1| putative membrane associated
protein [Arabidopsis thaliana]
gi|21592907|gb|AAM64857.1| putative membrane associated
protein [Arabidopsis thaliana]
Length = 295
Score = 114 bits (284), Expect(2) = 2e-29
Identities = 58/84 (69%), Positives = 66/84 (78%), Gaps = 3/84 (3%)
Frame = +2
Query: 233 HLHHHHHHNPNLHHPPPLEGSTASH---TSGSVSSVARSLLPTRRRLKLDPSSKLYFPYE 403
H HHH HH+ ++ P +GS + S SVSSVA+S LPT+RRLKLDPS KLYFPYE
Sbjct: 62 HQHHHQHHHQLGYNGPHGDGSGQNQHPTPSPSVSSVAKSFLPTKRRLKLDPSEKLYFPYE 121
Query: 404 PGKQVRSAIRIKNTSKSHVAFKFQ 475
PGKQVRSAI+IKNTSKSHVAFKFQ
Sbjct: 122 PGKQVRSAIKIKNTSKSHVAFKFQ 145
Score = 36.2 bits (82), Expect(2) = 2e-29
Identities = 18/43 (41%), Positives = 26/43 (59%), Gaps = 1/43 (2%)
Frame = +1
Query: 115 MAVAEPKPHSEPKV-WNLFKLPFRHSSTTANSTGTTSSSSTPS 240
M + E KP S+ K W FK+PFR+SS N+ + ++S PS
Sbjct: 1 MTMTEEKPTSDGKGGWGFFKIPFRNSSGHRNAASSAATSPFPS 43
>pir||T05153 hypothetical protein F18E5.70 - Arabidopsis thaliana
gi|3080389|emb|CAA18709.1| putative membrane associated
protein [Arabidopsis thaliana]
gi|7268942|emb|CAB81252.1| putative membrane associated
protein [Arabidopsis thaliana]
Length = 293
Score = 114 bits (284), Expect(2) = 2e-29
Identities = 58/84 (69%), Positives = 66/84 (78%), Gaps = 3/84 (3%)
Frame = +2
Query: 233 HLHHHHHHNPNLHHPPPLEGSTASH---TSGSVSSVARSLLPTRRRLKLDPSSKLYFPYE 403
H HHH HH+ ++ P +GS + S SVSSVA+S LPT+RRLKLDPS KLYFPYE
Sbjct: 62 HQHHHQHHHQLGYNGPHGDGSGQNQHPTPSPSVSSVAKSFLPTKRRLKLDPSEKLYFPYE 121
Query: 404 PGKQVRSAIRIKNTSKSHVAFKFQ 475
PGKQVRSAI+IKNTSKSHVAFKFQ
Sbjct: 122 PGKQVRSAIKIKNTSKSHVAFKFQ 145
Score = 36.2 bits (82), Expect(2) = 2e-29
Identities = 18/43 (41%), Positives = 26/43 (59%), Gaps = 1/43 (2%)
Frame = +1
Query: 115 MAVAEPKPHSEPKV-WNLFKLPFRHSSTTANSTGTTSSSSTPS 240
M + E KP S+ K W FK+PFR+SS N+ + ++S PS
Sbjct: 1 MTMTEEKPTSDGKGGWGFFKIPFRNSSGHRNAASSAATSPFPS 43
>dbj|BAA97133.1| membrane associated protein [Arabidopsis thaliana]
Length = 295
Score = 94.7 bits (234), Expect = 4e-19
Identities = 47/61 (77%), Positives = 52/61 (85%)
Frame = +2
Query: 293 STASHTSGSVSSVARSLLPTRRRLKLDPSSKLYFPYEPGKQVRSAIRIKNTSKSHVAFKF 472
S S ++SSVARSLLP RRRL+LDPSS LYFPYEPGKQVRSAI++KNTSKSH AFKF
Sbjct: 54 SAVSKPPLTMSSVARSLLPARRRLRLDPSSYLYFPYEPGKQVRSAIKLKNTSKSHTAFKF 113
Query: 473 Q 475
Q
Sbjct: 114 Q 114
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 418,687,115
Number of Sequences: 1393205
Number of extensions: 9629067
Number of successful extensions: 98065
Number of sequences better than 10.0: 620
Number of HSP's better than 10.0 without gapping: 60704
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 84504
length of database: 448,689,247
effective HSP length: 113
effective length of database: 291,257,082
effective search space used: 13106568690
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
