Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC008436A_C01 KMC008436A_c01
(559 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAA02747.1| membrane protein [Saccharum hybrid cultivar H65-7... 89 1e-26
ref|NP_196342.1| membrane protein; protein id: At5g07250.1, supp... 97 2e-26
gb|AAM92298.1| putative membrane protein [Oryza sativa (japonica... 89 9e-24
ref|NP_176500.1| membrane protein, putative; protein id: At1g631... 84 1e-23
ref|NP_172735.1| membrane protein, putative; protein id: At1g127... 78 2e-20
>gb|AAA02747.1| membrane protein [Saccharum hybrid cultivar H65-7052]
Length = 325
Score = 88.6 bits (218), Expect(3) = 1e-26
Identities = 40/71 (56%), Positives = 49/71 (68%)
Frame = -2
Query: 558 MLPHVDNFAHIGGFLAGFLLGFIFLPRPXFGWSEQQHLPTGVRVKSKYQAYPYVLWIVSA 379
+LPHVDNFAHIGGF GFLLGF+ L RP F W E+ LP KY+AY Y+LW+V+
Sbjct: 225 ILPHVDNFAHIGGFATGFLLGFVLLARPQFSWMERHELPQ-TNQPPKYKAYQYILWVVAL 283
Query: 378 VLFIAGLSIAL 346
VL + G I+L
Sbjct: 284 VLLLVGFVISL 294
Score = 42.7 bits (99), Expect(3) = 1e-26
Identities = 14/16 (87%), Positives = 15/16 (93%)
Frame = -1
Query: 301 WCHYLTCVPTSKWKCD 254
WCHYL CVPTSKWKC+
Sbjct: 309 WCHYLNCVPTSKWKCN 324
Score = 30.4 bits (67), Expect(3) = 1e-26
Identities = 12/21 (57%), Positives = 16/21 (76%)
Frame = -3
Query: 365 LGYPLPLVMLFCGENGYDHCH 303
+G+ + LVMLF G+NG D CH
Sbjct: 288 VGFVISLVMLFKGKNGNDGCH 308
>ref|NP_196342.1| membrane protein; protein id: At5g07250.1, supported by cDNA:
gi_16648761, supported by cDNA: gi_20466142 [Arabidopsis
thaliana] gi|11358506|pir||T48496 membrane protein -
Arabidopsis thaliana gi|7546703|emb|CAB87281.1| membrane
protein [Arabidopsis thaliana]
gi|16648762|gb|AAL25572.1| AT5g07250/T28J14_190
[Arabidopsis thaliana] gi|20466143|gb|AAM19993.1|
AT5g07250/T28J14_190 [Arabidopsis thaliana]
Length = 346
Score = 97.4 bits (241), Expect(2) = 2e-26
Identities = 41/71 (57%), Positives = 54/71 (75%)
Frame = -2
Query: 558 MLPHVDNFAHIGGFLAGFLLGFIFLPRPXFGWSEQQHLPTGVRVKSKYQAYPYVLWIVSA 379
+LPHVDNFAH+GGF+ GFLLGFI L RP F W ++H+P G ++ KY+ Y Y+LW++S
Sbjct: 244 ILPHVDNFAHVGGFVTGFLLGFILLARPQFKWLAREHMPQGTPLRYKYKTYQYLLWLLSL 303
Query: 378 VLFIAGLSIAL 346
VL IAG +AL
Sbjct: 304 VLLIAGFVVAL 314
Score = 43.5 bits (101), Expect(2) = 2e-26
Identities = 14/17 (82%), Positives = 15/17 (87%)
Frame = -1
Query: 301 WCHYLTCVPTSKWKCDD 251
WCHYL CVPTS W+CDD
Sbjct: 329 WCHYLRCVPTSSWRCDD 345
Score = 31.2 bits (69), Expect = 9.0
Identities = 15/33 (45%), Positives = 23/33 (69%)
Frame = -3
Query: 404 HMFYGLSLLFYSSLGYPLPLVMLFCGENGYDHC 306
++ + LSL+ + G+ + L+MLF GENG DHC
Sbjct: 296 YLLWLLSLVLLIA-GFVVALLMLFRGENGNDHC 327
>gb|AAM92298.1| putative membrane protein [Oryza sativa (japonica cultivar-group)]
Length = 329
Score = 89.0 bits (219), Expect(3) = 9e-24
Identities = 42/73 (57%), Positives = 55/73 (74%), Gaps = 1/73 (1%)
Frame = -2
Query: 558 MLPHVDNFAHIGGFLAGFLLGFIFLPRPXFGWSEQQHLPTGVRVKS-KYQAYPYVLWIVS 382
+LPHVDNFAHIGGFL GFLLGFIFL RP +GW ++ LP+ V+ S KY AY ++L V+
Sbjct: 227 ILPHVDNFAHIGGFLTGFLLGFIFLMRPHYGWMQRYVLPSSVKYTSKKYLAYQWILLAVA 286
Query: 381 AVLFIAGLSIALS 343
+VL + G ++ LS
Sbjct: 287 SVLAVIGFAVGLS 299
Score = 38.9 bits (89), Expect(3) = 9e-24
Identities = 11/15 (73%), Positives = 14/15 (93%)
Frame = -1
Query: 301 WCHYLTCVPTSKWKC 257
WCHYL+C+PTS+W C
Sbjct: 313 WCHYLSCIPTSRWTC 327
Score = 23.9 bits (50), Expect(3) = 9e-24
Identities = 9/21 (42%), Positives = 13/21 (61%)
Frame = -3
Query: 365 LGYPLPLVMLFCGENGYDHCH 303
+G+ + L MLF G N + CH
Sbjct: 292 IGFAVGLSMLFRGVNANERCH 312
>ref|NP_176500.1| membrane protein, putative; protein id: At1g63120.1 [Arabidopsis
thaliana] gi|25404407|pir||F96656 probable membrane
protein F16M19.4 [imported] - Arabidopsis thaliana
gi|12323258|gb|AAG51610.1|AC010795_14 membrane protein,
putative; 61952-60281 [Arabidopsis thaliana]
gi|28393358|gb|AAO42103.1| putative membrane protein
[Arabidopsis thaliana] gi|28827620|gb|AAO50654.1|
putative membrane protein [Arabidopsis thaliana]
Length = 317
Score = 84.3 bits (207), Expect(3) = 1e-23
Identities = 40/71 (56%), Positives = 48/71 (67%)
Frame = -2
Query: 558 MLPHVDNFAHIGGFLAGFLLGFIFLPRPXFGWSEQQHLPTGVRVKSKYQAYPYVLWIVSA 379
MLP VDNFAHIGGFL GF LGF+ L RP +GW + R K KY Y YVL++VS
Sbjct: 220 MLPRVDNFAHIGGFLTGFCLGFVLLVRPQYGWEASR--TNTSRTKRKYSMYQYVLFVVSV 277
Query: 378 VLFIAGLSIAL 346
VL + GL++AL
Sbjct: 278 VLLVVGLTVAL 288
Score = 38.5 bits (88), Expect(3) = 1e-23
Identities = 12/15 (80%), Positives = 13/15 (86%)
Frame = -1
Query: 301 WCHYLTCVPTSKWKC 257
WCHYL+C PTSKW C
Sbjct: 303 WCHYLSCFPTSKWTC 317
Score = 28.5 bits (62), Expect(3) = 1e-23
Identities = 12/20 (60%), Positives = 14/20 (70%)
Frame = -3
Query: 365 LGYPLPLVMLFCGENGYDHC 306
+G + LVMLF GENG HC
Sbjct: 282 VGLTVALVMLFKGENGNKHC 301
>ref|NP_172735.1| membrane protein, putative; protein id: At1g12750.1, supported by
cDNA: gi_17529033 [Arabidopsis thaliana]
gi|17529034|gb|AAL38727.1| putative membrane protein
[Arabidopsis thaliana] gi|20259083|gb|AAM14257.1|
putative membrane protein [Arabidopsis thaliana]
Length = 307
Score = 77.8 bits (190), Expect(3) = 2e-20
Identities = 39/72 (54%), Positives = 48/72 (66%), Gaps = 1/72 (1%)
Frame = -2
Query: 558 MLPHVDNFAHIGGFLAGFLLGFIFLPRPXFGWSE-QQHLPTGVRVKSKYQAYPYVLWIVS 382
+LP VDNFAHIGG L GF LGFI L +P GW E + G R +SKY YVL+ V+
Sbjct: 207 LLPWVDNFAHIGGLLTGFCLGFILLMQPQSGWEEFRNSSQYGARARSKYNPCQYVLFFVA 266
Query: 381 AVLFIAGLSIAL 346
AVL +AGL++ L
Sbjct: 267 AVLVVAGLTVGL 278
Score = 34.7 bits (78), Expect(3) = 2e-20
Identities = 11/15 (73%), Positives = 11/15 (73%)
Frame = -1
Query: 301 WCHYLTCVPTSKWKC 257
WCH L C PTSKW C
Sbjct: 293 WCHRLDCYPTSKWSC 307
Score = 27.7 bits (60), Expect(3) = 2e-20
Identities = 12/19 (63%), Positives = 13/19 (68%)
Frame = -3
Query: 362 GYPLPLVMLFCGENGYDHC 306
G + LVMLF GENG HC
Sbjct: 273 GLTVGLVMLFDGENGNKHC 291
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 501,347,457
Number of Sequences: 1393205
Number of extensions: 11323114
Number of successful extensions: 29829
Number of sequences better than 10.0: 60
Number of HSP's better than 10.0 without gapping: 28630
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 29739
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 19808345223
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
