KMC008337A_c01
[Fasta Sequence]   [Nr Search]   [EST assemble image]  

Fasta Sequence
>KMC008337A_C01 KMC008337A_c01
gagcaaatatggggagcagtgGGTGATAAAATTATCGTACAAGAATAGAGGAACTACAAA
ATAGCTGAAAACTATAGAACCAAAATGCTGACACATATTCCCAACATAGAACTGAAAGCT
AAATACATTATGGTGCATAAACGCCTAAAAGGCATAGTAAAAATATAATATCACAATGAC
CTGGAATCATTCTACAAAAATTATATTTGGGCAAGTATTAACAACCAAGAGTGAAACAAA
TCATTTTCTCACTTCCCTCCGCATAAAGGAAGCACCATACTCCAACGATCAAAAAGGTTT
CTCGCTCTCGTTTAACATCTGATGGTTGCCTGTTAGAATTACAAGGCACTTTCACTATTC
AAGCCTGCAAGCCTAACTTTATACCCAAAATTAAATTCACTTGTACTCGAGATCGGGCAC
AGGAGGGGCATCAAAGCTCTCCAAAACCTTGGTCTCACTTCCTCCATCACCACCAGCTGC
AACCTCATCCTTCTTCCCAAACCACCCACCACCGAACCACGAATTCTGCGGCGGCGC


Nr search

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC008337A_C01 KMC008337A_c01
         (537 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_181277.1| putative protein translocase; protein id: At2g3...    52  6e-06
gb|AAF03749.1|AF186847_1 TIM17 [Arabidopsis thaliana]                  51  8e-06
ref|NP_173460.1| mitochondrial inner membrane translocase compon...    39  0.052
ref|NP_594630.1| putative histone acetyl transferase [Schizosacc...    34  0.98
ref|NP_478441.1| transposase [Nostoc sp. PCC 7120] gi|25535728|p...    33  1.7

>ref|NP_181277.1| putative protein translocase; protein id: At2g37410.1, supported by
           cDNA: gi_13430691, supported by cDNA: gi_15810185,
           supported by cDNA: gi_6103721 [Arabidopsis thaliana]
           gi|12643851|sp|Q9SP35|IM17_ARATH Mitochondrial import
           inner membrane translocase subunit TIM17
           gi|25408549|pir||D84792 probable protein translocase
           [imported] - Arabidopsis thaliana
           gi|4056494|gb|AAC98060.1| putative protein translocase
           [Arabidopsis thaliana]
           gi|13430692|gb|AAK25968.1|AF360258_1 putative protein
           translocase [Arabidopsis thaliana]
           gi|14532896|gb|AAK64130.1| putative protein translocase
           [Arabidopsis thaliana] gi|15810186|gb|AAL06994.1|
           At2g37410/F3G5.20 [Arabidopsis thaliana]
          Length = 243

 Score = 51.6 bits (122), Expect = 6e-06
 Identities = 25/42 (59%), Positives = 32/42 (75%)
 Frame = -1

Query: 525 NSWFGGGWFGKKDEVAAGGDGGSETKVLESFDAPPVPDLEYK 400
           +SWFGG +  KK+EV  G +  S+T+VLESFDAPPVP  E+K
Sbjct: 204 SSWFGGLFDKKKEEVQPGSE--SKTEVLESFDAPPVPSFEFK 243

>gb|AAF03749.1|AF186847_1 TIM17 [Arabidopsis thaliana]
          Length = 243

 Score = 51.2 bits (121), Expect = 8e-06
 Identities = 25/42 (59%), Positives = 32/42 (75%)
 Frame = -1

Query: 525 NSWFGGGWFGKKDEVAAGGDGGSETKVLESFDAPPVPDLEYK 400
           +SWFGG +  KK+EV  G +  S+T+VLESFDAPPVP  E+K
Sbjct: 204 SSWFGGLFDKKKEEVHPGSE--SKTEVLESFDAPPVPSFEFK 243

>ref|NP_173460.1| mitochondrial inner membrane translocase component, putative;
           protein id: At1g20350.1 [Arabidopsis thaliana]
           gi|25332422|pir||C86337 hypothetical protein F14O10.5 -
           Arabidopsis thaliana gi|9558591|gb|AAF88154.1|AC026234_5
           Contains similarity to a mitochondrial inner membrane
           translocase component Tim17b from Mus musculus
           gb|AF106621.  EST gb|AI998083 comes from this gene.
           [Arabidopsis thaliana] gi|26451931|dbj|BAC43058.1|
           unknown protein [Arabidopsis thaliana]
           gi|28950759|gb|AAO63303.1| At1g20350 [Arabidopsis
           thaliana]
          Length = 218

 Score = 38.5 bits (88), Expect = 0.052
 Identities = 20/41 (48%), Positives = 24/41 (57%)
 Frame = -1

Query: 522 SWFGGGWFGKKDEVAAGGDGGSETKVLESFDAPPVPDLEYK 400
           SWFG  +  KK +        S T +LESFDAPPVP  E+K
Sbjct: 180 SWFGSLF--KKKKETEDHHSESRTHILESFDAPPVPTYEFK 218

>ref|NP_594630.1| putative histone acetyl transferase [Schizosaccharomyces pombe]
           gi|7492650|pir||T39004 probable histone acetyl
           transferase - fission yeast  (Schizosaccharomyces pombe)
           gi|4056556|emb|CAA22591.1| putative histone acetyl
           transferase [Schizosaccharomyces pombe]
          Length = 463

 Score = 34.3 bits (77), Expect = 0.98
 Identities = 18/65 (27%), Positives = 33/65 (50%)
 Frame = +1

Query: 73  YRTKMLTHIPNIELKAKYIMVHKRLKGIVKI*YHNDLESFYKNYIWASINNQE*NKSFSH 252
           Y++K+         KA+ +M+ KR +G+V   ++ND         W +I+N + +K   +
Sbjct: 23  YKSKVFAFKDGEYRKAEILMIQKRTRGVVYYVHYNDYNKRLDE--WITIDNIDLSKGIEY 80

Query: 253 FPPHK 267
            PP K
Sbjct: 81  PPPEK 85

>ref|NP_478441.1| transposase [Nostoc sp. PCC 7120] gi|25535728|pir||AI2552
           transposase alr8016 [imported] - Nostoc sp. (strain PCC
           7120) plasmid pCC7120gamma gi|17134789|dbj|BAB77346.1|
           transposase [Nostoc sp. PCC 7120]
          Length = 636

 Score = 33.5 bits (75), Expect = 1.7
 Identities = 19/54 (35%), Positives = 28/54 (51%), Gaps = 1/54 (1%)
 Frame = +1

Query: 370 SLTLYPKLNSLVLEIGHRRGIKALQNLGLTSSITTSCNLI-LLPKPPTTEPRIL 528
           +L L  +LN++ L  G   G++  Q LG+  S  T  NL+  +P PP   P  L
Sbjct: 105 TLRLAQRLNAIALANGGAAGVRLSQQLGIKVSRNTLLNLVRSIPLPPIVTPHTL 158

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 491,080,907
Number of Sequences: 1393205
Number of extensions: 10584014
Number of successful extensions: 47293
Number of sequences better than 10.0: 27
Number of HSP's better than 10.0 without gapping: 40062
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 46854
length of database: 448,689,247
effective HSP length: 115
effective length of database: 288,470,672
effective search space used: 18173652336
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)


EST assemble image


clone accession position
1 SPD075a11_f BP049961 1 314
2 SPD026e02_f BP046062 22 542
3 GNf017a12 BP068576 25 448




Lotus japonicus
Kazusa DNA Research Institute