Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC007528A_C01 KMC007528A_c01
(581 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_179682.1| lipoic acid synthase (LIP1); protein id: At2g20... 241 5e-63
dbj|BAB44157.1| lipoic acid synthase [Bruguiera gymnorrhiza] 231 6e-60
gb|EAA18375.1| lipoic acid synthetase, putative [Plasmodium yoel... 149 2e-35
ref|NP_077791.1| lipoic acid synthetase [Mus musculus] gi|238218... 147 9e-35
ref|XP_047493.4| similar to LD24887p [Drosophila melanogaster] [... 145 5e-34
>ref|NP_179682.1| lipoic acid synthase (LIP1); protein id: At2g20860.1, supported by
cDNA: gi_16323377, supported by cDNA: gi_3928757
[Arabidopsis thaliana] gi|11279594|pir||T44259 lipoic
acid synthase (EC 2.8.1.-) [imported] - Arabidopsis
thaliana gi|3928758|dbj|BAA34701.1| Lipoic acid synthase
[Arabidopsis thaliana] gi|4454462|gb|AAD20909.1| lipoic
acid synthase (LIP1) [Arabidopsis thaliana]
gi|14334940|gb|AAK59647.1| putative lipoic acid synthase
LIP1 [Arabidopsis thaliana]
gi|15983418|gb|AAL11577.1|AF424583_1 At2g20860/F5H14.17
[Arabidopsis thaliana] gi|16323378|gb|AAL15183.1|
putative lipoic acid synthase LIP1 [Arabidopsis
thaliana]
Length = 374
Score = 241 bits (615), Expect = 5e-63
Identities = 116/132 (87%), Positives = 127/132 (95%)
Frame = -2
Query: 580 ELQSVVRDHRANFKQSLDVLMMAKDYAPAGTLTKTSIMLGCGETPDQVLKTMEKVRAAGV 401
ELQS VRDHRANFKQSLDVL MAK+YAPAGTLTKTS+MLGCGETPDQV+KTMEKVRAAGV
Sbjct: 240 ELQSFVRDHRANFKQSLDVLRMAKEYAPAGTLTKTSVMLGCGETPDQVVKTMEKVRAAGV 299
Query: 400 DVMTFGQYMRPSKRHMPVSEYITPEAFEKYQTLGMEMGFRYVASGPMVRSSYKAGEFYIK 221
DVMTFGQYMRPSKRHMPV+EY+TP+AFE+Y+ LGMEMGFRYVASGPMVRSSYKAGE+YIK
Sbjct: 300 DVMTFGQYMRPSKRHMPVAEYVTPDAFERYRLLGMEMGFRYVASGPMVRSSYKAGEYYIK 359
Query: 220 SMIDSDRAASSS 185
SMI++DR AS S
Sbjct: 360 SMIEADRVASPS 371
>dbj|BAB44157.1| lipoic acid synthase [Bruguiera gymnorrhiza]
Length = 375
Score = 231 bits (588), Expect = 6e-60
Identities = 112/133 (84%), Positives = 127/133 (95%)
Frame = -2
Query: 580 ELQSVVRDHRANFKQSLDVLMMAKDYAPAGTLTKTSIMLGCGETPDQVLKTMEKVRAAGV 401
ELQSVVRD+RANFKQSL+VLM A++++P GTLTKTSIMLGCGETPDQV+K+MEKVRAAGV
Sbjct: 238 ELQSVVRDNRANFKQSLEVLMAAREFSPPGTLTKTSIMLGCGETPDQVVKSMEKVRAAGV 297
Query: 400 DVMTFGQYMRPSKRHMPVSEYITPEAFEKYQTLGMEMGFRYVASGPMVRSSYKAGEFYIK 221
DV+TFGQYMRPSKRHMPVSEYITPEAFEKY+ LG EMGFRYVASGPMVRSSYKAGEFYIK
Sbjct: 298 DVITFGQYMRPSKRHMPVSEYITPEAFEKYKVLGDEMGFRYVASGPMVRSSYKAGEFYIK 357
Query: 220 SMIDSDRAASSSK 182
+MI+ DRAA++ +
Sbjct: 358 NMIERDRAAAAQQ 370
>gb|EAA18375.1| lipoic acid synthetase, putative [Plasmodium yoelii yoelii]
Length = 502
Score = 149 bits (377), Expect = 2e-35
Identities = 71/133 (53%), Positives = 101/133 (75%)
Frame = -2
Query: 577 LQSVVRDHRANFKQSLDVLMMAKDYAPAGTLTKTSIMLGCGETPDQVLKTMEKVRAAGVD 398
LQ VRD RAN++QSL VL AK+ P TKTSIMLG GET D+VL+TM+ R+ +D
Sbjct: 325 LQKYVRDKRANYEQSLYVLKKAKEINP-NLYTKTSIMLGLGETQDEVLQTMKDARSNDID 383
Query: 397 VMTFGQYMRPSKRHMPVSEYITPEAFEKYQTLGMEMGFRYVASGPMVRSSYKAGEFYIKS 218
V+TFGQY+RP+K H+ V EYI+P+ F Y+ +G++MGF+Y+ASGP+VRSSY AGE+++K+
Sbjct: 384 VITFGQYLRPTKNHLNVVEYISPQMFNYYKDVGLKMGFKYIASGPLVRSSYMAGEYFMKN 443
Query: 217 MIDSDRAASSSKL 179
M++ R + ++
Sbjct: 444 MVEKGRNQKNQQI 456
>ref|NP_077791.1| lipoic acid synthetase [Mus musculus]
gi|23821835|sp|Q99M04|LIAS_MOUSE Lipoic acid synthetase,
mitochondrial precursor (Lip-syn) (Lipoate synthase)
(mLIP1) gi|12805345|gb|AAH02141.1| Unknown (protein for
MGC:7254) [Mus musculus] gi|14669826|dbj|BAB62009.1|
lipoic acid synthase [Mus musculus]
gi|26339544|dbj|BAC33443.1| unnamed protein product [Mus
musculus] gi|26340282|dbj|BAC33804.1| unnamed protein
product [Mus musculus] gi|26346042|dbj|BAC36672.1|
unnamed protein product [Mus musculus]
Length = 373
Score = 147 bits (371), Expect = 9e-35
Identities = 75/134 (55%), Positives = 101/134 (74%)
Frame = -2
Query: 580 ELQSVVRDHRANFKQSLDVLMMAKDYAPAGTLTKTSIMLGCGETPDQVLKTMEKVRAAGV 401
ELQ VRD RANF QSL VL AK+ P ++KTSIMLG GET +QV T++ +RAA V
Sbjct: 242 ELQRKVRDPRANFDQSLRVLRHAKEVQP-DVVSKTSIMLGLGETDEQVYATLKALRAADV 300
Query: 400 DVMTFGQYMRPSKRHMPVSEYITPEAFEKYQTLGMEMGFRYVASGPMVRSSYKAGEFYIK 221
D +T GQYM+P+KRH+ V EY+TPE F+ ++ +G E+GF Y ASGP+VRSSYKAGEF++K
Sbjct: 301 DCLTLGQYMQPTKRHLKVEEYVTPEKFKYWEKVGNELGFLYTASGPLVRSSYKAGEFFLK 360
Query: 220 SMIDSDRAASSSKL 179
+++ + R +SK+
Sbjct: 361 NLV-ARRKTKASKV 373
>ref|XP_047493.4| similar to LD24887p [Drosophila melanogaster] [Homo sapiens]
Length = 372
Score = 145 bits (365), Expect = 5e-34
Identities = 72/123 (58%), Positives = 93/123 (75%)
Frame = -2
Query: 580 ELQSVVRDHRANFKQSLDVLMMAKDYAPAGTLTKTSIMLGCGETPDQVLKTMEKVRAAGV 401
ELQS VRD RANF QSL VL AK P ++KTSIMLG GE +QV TM+ +R A V
Sbjct: 243 ELQSKVRDPRANFDQSLRVLKHAKKVQP-DVISKTSIMLGLGENDEQVYATMKALREADV 301
Query: 400 DVMTFGQYMRPSKRHMPVSEYITPEAFEKYQTLGMEMGFRYVASGPMVRSSYKAGEFYIK 221
D +T GQYM+P++RH+ V EYITPE F+ ++ +G E+GF Y ASGP+VRSSYKAGEF++K
Sbjct: 302 DCLTLGQYMQPTRRHLKVEEYITPEKFKYWEKVGNELGFHYTASGPLVRSSYKAGEFFLK 361
Query: 220 SMI 212
+++
Sbjct: 362 NLV 364
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 514,426,538
Number of Sequences: 1393205
Number of extensions: 11505137
Number of successful extensions: 26841
Number of sequences better than 10.0: 154
Number of HSP's better than 10.0 without gapping: 26085
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 26789
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 21712003912
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
