Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC007454A_C01 KMC007454A_c01
(614 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_199475.1| AP47/50p (gb|AAB88283.1); protein id: At5g46630... 161 7e-39
sp|P54672|AP50_DICDI CLATHRIN COAT ASSEMBLY PROTEIN AP50 (CLATHR... 108 4e-23
gb|AAO51241.1| similar to Dictyostelium discoideum (Slime mold).... 108 4e-23
gb|EAA31527.1| hypothetical protein [Neurospora crassa] gi|28949... 103 2e-21
ref|NP_732744.1| CG7057-PA [Drosophila melanogaster] gi|28571823... 86 3e-16
>ref|NP_199475.1| AP47/50p (gb|AAB88283.1); protein id: At5g46630.1, supported by
cDNA: gi_14326519, supported by cDNA: gi_2271476
[Arabidopsis thaliana] gi|2271477|gb|AAB88283.1|
AP47/50p [Arabidopsis thaliana]
gi|9758499|dbj|BAB08907.1| AP47/50p [Arabidopsis
thaliana]
Length = 438
Score = 161 bits (407), Expect = 7e-39
Identities = 77/80 (96%), Positives = 78/80 (97%)
Frame = -2
Query: 613 KIRKFPGQTEPTLSAEVELISTMTEKKSWTRPPIQMEFQVPMFTASGLRVRFLKVWEKSG 434
KIRKFPGQTE TLSAE+ELISTM EKKSWTRPPIQMEFQVPMFTASGLRVRFLKVWEKSG
Sbjct: 359 KIRKFPGQTESTLSAEIELISTMGEKKSWTRPPIQMEFQVPMFTASGLRVRFLKVWEKSG 418
Query: 433 YNTVEWVRYITKAGSYEIRC 374
YNTVEWVRYITKAGSYEIRC
Sbjct: 419 YNTVEWVRYITKAGSYEIRC 438
>sp|P54672|AP50_DICDI CLATHRIN COAT ASSEMBLY PROTEIN AP50 (CLATHRIN COAT ASSOCIATED
PROTEIN AP50) (PLASMA MEMBRANE ADAPTOR AP-2 50 KD
PROTEIN) (HA2 50 KD SUBUNIT) (CLATHRIN ASSEMBLY PROTEIN
COMPLEX 2 MEDIUM CHAIN) gi|7448829|pir||JC5719
clathrin-associated adaptor complex medium chain - slime
mold (Dictyostelium discoideum)
gi|1174262|gb|AAB41282.1| DdApm1
Length = 439
Score = 108 bits (271), Expect = 4e-23
Identities = 52/80 (65%), Positives = 66/80 (82%), Gaps = 1/80 (1%)
Frame = -2
Query: 613 KIRKFPGQTEPTLSAEVELISTMT-EKKSWTRPPIQMEFQVPMFTASGLRVRFLKVWEKS 437
+IR+FPG TE TL AEVEL++++ +KK+W+RPPI MEFQV MFTASG VRFLKV EKS
Sbjct: 359 RIRRFPGDTEFTLRAEVELMASVNLDKKAWSRPPISMEFQVTMFTASGFSVRFLKVVEKS 418
Query: 436 GYNTVEWVRYITKAGSYEIR 377
Y ++WVRY+TKAG+Y+ R
Sbjct: 419 NYTPIKWVRYLTKAGTYQNR 438
>gb|AAO51241.1| similar to Dictyostelium discoideum (Slime mold). Clathrin coat
assembly protein AP50 (Clathrin coat associated protein
AP50) (Plasma membrane adaptor AP-2 50 kDa protein) (HA2
50 kDa subunit) (Clathrin assembly protein complex 2
medium chain)
Length = 444
Score = 108 bits (271), Expect = 4e-23
Identities = 52/80 (65%), Positives = 66/80 (82%), Gaps = 1/80 (1%)
Frame = -2
Query: 613 KIRKFPGQTEPTLSAEVELISTMT-EKKSWTRPPIQMEFQVPMFTASGLRVRFLKVWEKS 437
+IR+FPG TE TL AEVEL++++ +KK+W+RPPI MEFQV MFTASG VRFLKV EKS
Sbjct: 364 RIRRFPGDTEFTLRAEVELMASVNLDKKAWSRPPISMEFQVTMFTASGFSVRFLKVVEKS 423
Query: 436 GYNTVEWVRYITKAGSYEIR 377
Y ++WVRY+TKAG+Y+ R
Sbjct: 424 NYTPIKWVRYLTKAGTYQNR 443
>gb|EAA31527.1| hypothetical protein [Neurospora crassa]
gi|28949978|emb|CAD70739.1| probable clathrin-associated
adaptor complex medium chain [Neurospora crassa]
Length = 436
Score = 103 bits (256), Expect = 2e-21
Identities = 50/79 (63%), Positives = 65/79 (81%)
Frame = -2
Query: 613 KIRKFPGQTEPTLSAEVELISTMTEKKSWTRPPIQMEFQVPMFTASGLRVRFLKVWEKSG 434
KI +F GQ+E LSAE EL S MT +K+W+RPP+ M F + MFT+SGL VR+LKV+EKS
Sbjct: 359 KIGRFAGQSEFVLSAEAELTS-MTNQKAWSRPPLSMNFSLLMFTSSGLLVRYLKVFEKSN 417
Query: 433 YNTVEWVRYITKAGSYEIR 377
Y++V+WVRY+T+AGSYEIR
Sbjct: 418 YSSVKWVRYMTRAGSYEIR 436
>ref|NP_732744.1| CG7057-PA [Drosophila melanogaster] gi|28571823|ref|NP_651049.3|
CG7057-PB [Drosophila melanogaster]
gi|3150082|emb|CAA06785.1| clathrin-associated protein
[Drosophila melanogaster]
gi|6492274|gb|AAF14248.1|AF110232_1 clathrin-associated
adaptor complex AP-2 medium chain [Drosophila
melanogaster] gi|7300860|gb|AAF56001.1| CG7057-PA
[Drosophila melanogaster] gi|17944592|gb|AAL48183.1|
SD05403p [Drosophila melanogaster]
gi|28381420|gb|AAF56002.3| CG7057-PB [Drosophila
melanogaster]
Length = 437
Score = 86.3 bits (212), Expect = 3e-16
Identities = 45/84 (53%), Positives = 59/84 (69%), Gaps = 4/84 (4%)
Frame = -2
Query: 613 KIRKFPGQTEPTLSAEVELISTMTEKKSWTRPPIQMEFQVPMFTASGLRVRFLKVWEK-- 440
KI++ G E LSAE+EL+ T T+KK WTRPPI M F+VP F SG +VR+LKV+E
Sbjct: 356 KIKRMAGMKETQLSAEIELLETDTKKK-WTRPPISMNFEVP-FAPSGFKVRYLKVFEPKL 413
Query: 439 --SGYNTVEWVRYITKAGSYEIRC 374
S ++ V+WVRYI ++G YE RC
Sbjct: 414 NYSDHDVVKWVRYIGRSGLYETRC 437
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 505,592,412
Number of Sequences: 1393205
Number of extensions: 10848930
Number of successful extensions: 24675
Number of sequences better than 10.0: 96
Number of HSP's better than 10.0 without gapping: 24054
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 24598
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 24854530794
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
